Homeotic gene expression in the wild-type and a homeotic mutant of the moth Manduca sexta

ABSTRACT We report on differential gene expression in the cyanobacterium Synechocystis sp. strain PCC 6803 after light-dark transitions in wild-type, ΔsigB, and ΔsigD strains. We also studied the effect of day length in the presence of glucose on a ΔsigB ΔsigE mutant. Our results indicated that the absence of SigB or SigD predominately altered gene expression in the dark or in the light, respectively. In the light, approximately 350 genes displayed transcript levels in the ΔsigD strain that were different from those of the wild type, with over 200 of these up-regulated in the mutant. In the dark, removal of SigB altered more than 150 genes, and the levels of 136 of these were increased in the mutant compared to those in the wild type. The removal of both SigB and SigE had a major impact on gene expression under mixotrophic growth conditions and resulted in the inability of cells to grow in the presence of glucose with 8-h light and 16-h dark cycles. Our results indicated the importance of group II σ factors in the global regulation of transcription in this organism and are best explained by using the σ cycle paradigm with the stochastic release model described previously (R. A. Mooney, S. A. Darst, and R. Landick, Mol. Cell 20:335-345, 2005). We combined our results with the total protein levels of the σ factors in the light and dark as calculated previously (S. Imamura, S. Yoshihara, S. Nakano, N. Shiozaki, A. Yamada, K. Tanaka, H. Takahashi, M. Asayama, and M. Shirai, J. Mol. Biol. 325:857-872, 2003; S. Imamura, M. Asayama, H. Takahashi, K. Tanaka, H. Takahashi, and M. Shirai, FEBS Lett. 554:357-362, 2003). Thus, we concluded that the control of global transcription is based on the amount of the various σ factors present and able to bind RNA polymerase.

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SET1A/COMPASS and shadow enhancers in the regulation of homeotic gene expression

Genes & Development ◽

10.1101/gad.294744.116 ◽

2017 ◽

Vol 31 (8) ◽

pp. 787-801 ◽

Cited By ~ 26

Author(s):

Kaixiang Cao ◽

Clayton K. Collings ◽

Stacy A. Marshall ◽

Marc A. Morgan ◽

Emily J. Rendleman ◽

...

Keyword(s):

Gene Expression ◽

Homeotic Gene ◽

Homeotic Gene Expression

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The development of the sensory neuron pattern in the antennal disc of wild-type and mutant (lz3, ssa) Drosophila melanogaster

Development ◽

10.1242/dev.112.4.1063 ◽

1991 ◽

Vol 112 (4) ◽

pp. 1063-1075

Author(s):

M.C. Lienhard ◽

R.F. Stocker

Keyword(s):

Drosophila Melanogaster ◽

Sensory Neuron ◽

Antennal Segment ◽

Wild Type ◽

Homeotic Mutant ◽

In The Wild ◽

The Brain ◽

Antennal Disc ◽

Antennal Nerve

The development of the sensory neuron pattern in the antennal disc of Drosophila melanogaster was studied with a neuron-specific monoclonal antibody (22C10). In the wild type, the earliest neurons become visible 3 h after pupariation, much later than in other imaginal discs. They lie in the center of the disc and correspond to the neurons of the adult aristal sensillum. Their axons join the larval antennal nerve and seem to establish the first connection towards the brain. Later on, three clusters of neurons appear in the periphery of the disc. Two of them most likely give rise to the Johnston's organ in the second antennal segment. Neurons of the olfactory third antennal segment are formed only after eversion of the antennal disc (clusters t1-t3). The adult pattern of antennal neurons is established at about 27% of metamorphosis. In the mutant lozenge3 (lz3), which lacks basiconic antennal sensilla, cluster t3 fails to develop. This indicates that, in the wild type, a homogeneous group of basiconic sensilla is formed by cluster t3. The possible role of the lozenge gene in sensillar determination is discussed. The homeotic mutant spineless-aristapedia (ssa) transforms the arista into a leg-like tarsus. Unlike leg discs, neurons are missing in the larval antennal disc of ssa. However, the first neurons differentiate earlier than in normal antennal discs. Despite these changes, the pattern of afferents in the ectopic tarsus appears leg specific, whereas in the non-transformed antennal segments a normal antennal pattern is formed. This suggests that neither larval leg neurons nor early aristal neurons are essential for the outgrowth of subsequent afferents.

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Homeotic gene expression in the locustSchistocerca: An antibody that detects conserved epitopes in ultrabithorax and abdominal-A proteins

Developmental Genetics ◽

10.1002/dvg.1020150104 ◽

1994 ◽

Vol 15 (1) ◽

pp. 19-31 ◽

Cited By ~ 69

Author(s):

Robert Kelsh ◽

Robert O. J. Weinzierl ◽

Robert A. H. White ◽

Michael Akam

Keyword(s):

Gene Expression ◽

Homeotic Gene ◽

Homeotic Gene Expression

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Carvedilol Induces Changes In Gene Expression In The Wild-Type Rat Heart

10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a3457 ◽

2012 ◽

Author(s):

Jennifer I. Drake ◽

Ramesh Natarajan ◽

Harm J. Bogaard ◽

Donatas Kraskauskas ◽

Norbert F. Voelkel

Keyword(s):

Gene Expression ◽

Rat Heart ◽

Wild Type ◽

In The Wild

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Effect of Metallothionein-III on Mercury-Induced Chemokine Gene Expression

Toxics ◽

10.3390/toxics6030048 ◽

2018 ◽

Vol 6 (3) ◽

pp. 48 ◽

Cited By ~ 2

Author(s):

Jin-Yong Lee ◽

Maki Tokumoto ◽

Gi-Wook Hwang ◽

Min-Seok Kim ◽

Tsutomu Takahashi ◽

...

Keyword(s):

Gene Expression ◽

Molecular Mechanisms ◽

Mercury Vapor ◽

Brain Diseases ◽

Wild Type ◽

Chemokine Gene ◽

Mercury Compounds ◽

In The Wild ◽

The Brain ◽

Chemokine Gene Expression

Mercury compounds are known to cause central nervous system disorders; however the detailed molecular mechanisms of their actions remain unclear. Methylmercury increases the expression of several chemokine genes, specifically in the brain, while metallothionein-III (MT-III) has a protective role against various brain diseases. In this study, we investigated the involvement of MT-III in chemokine gene expression changes in response to methylmercury and mercury vapor in the cerebrum and cerebellum of wild-type mice and MT-III null mice. No difference in mercury concentration was observed between the wild-type mice and MT-III null mice in any brain tissue examined. The expression of Ccl3 in the cerebrum and of Cxcl10 in the cerebellum was increased by methylmercury in the MT-III null but not the wild-type mice. The expression of Ccl7 in the cerebellum was increased by mercury vapor in the MT-III null mice but not the wild-type mice. However, the expression of Ccl12 and Cxcl12 was increased in the cerebrum by methylmercury only in the wild-type mice and the expression of Ccl3 in the cerebellum was increased by mercury vapor only in the wild-type mice. These results indicate that MT-III does not affect mercury accumulation in the brain, but that it affects the expression of some chemokine genes in response to mercury compounds.

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Author response: Deregulated FGF and homeotic gene expression underlies cerebellar vermis hypoplasia in CHARGE syndrome

10.7554/elife.01305.012 ◽

2013 ◽

Author(s):

Tian Yu ◽

Linda C Meiners ◽

Katrin Danielsen ◽

Monica TY Wong ◽

Timothy Bowler ◽

...

Keyword(s):

Gene Expression ◽

Charge Syndrome ◽

Cerebellar Vermis ◽

Author Response ◽

Homeotic Gene ◽

Homeotic Gene Expression

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Molecular characterisation of the Polycomblike gene of Drosophila melanogaster, a trans-acting negative regulator of homeotic gene expression

Development ◽

10.1242/dev.120.9.2629 ◽

1994 ◽

Vol 120 (9) ◽

pp. 2629-2636 ◽

Cited By ~ 19

Author(s):

A. Lonie ◽

R. D'Andrea ◽

R. Paro ◽

R. Saint

Keyword(s):

Gene Expression ◽

Drosophila Melanogaster ◽

Polycomb Group ◽

Negative Regulator ◽

Homeotic Gene ◽

Homeotic Genes ◽

Molecular Characterisation ◽

Polycomb Group Proteins ◽

Polycomb Group Genes ◽

Homeotic Gene Expression

The Polycomblike gene of Drosophila melanogaster, a member of the Polycomb Group of genes, is required for the correct spatial expression of the homeotic genes of the Antennapaedia and Bithorax Complexes. Mutations in Polycomb Group genes result in ectopic homeotic gene expression, indicating that Polycomb Group proteins maintain the transcriptional repression of specific homeotic genes in specific tissues during development. We report here the isolation and molecular characterisation of the Polycomblike gene. The Polycomblike transcript encodes an 857 amino acid protein with no significant homology to other proteins. Antibodies raised against the product of this open reading frame were used to show that the Polycomblike protein is found in all nuclei during embryonic development. Antibody staining also revealed that the Polycomblike protein is found on larval salivary gland polytene chromosomes at about 100 specific loci, the same loci to which the Polycomb and polyhomeotic proteins, two other Polycomb Group proteins, are found. These data add further support for a model in which Polycomb Group proteins form multimeric protein complexes at specific chromosomal loci to repress transcription at those loci.

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