Differential induction of cinnamyl alcohol dehydrogenase during defensive lignification in wheat ( Triticum aestivum L.): characterisation of the major inducible form

Planta ◽  
1999 ◽  
Vol 208 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Heidi J. Mitchell ◽  
Sharon A. Hall ◽  
Rebecca Stratford ◽  
John L. Hall ◽  
Mark S. Barber
Keyword(s):  
Triticum Aestivum ◽  
Alcohol Dehydrogenase ◽  
Cinnamyl Alcohol Dehydrogenase ◽  
Triticum Aestivum L ◽  
Cinnamyl Alcohol ◽  
Differential Induction

Comparative efficiency of different constructs for down regulation of tobacco cinnamyl alcohol dehydrogenase

1998 ◽  
Vol 49 (2) ◽  
pp. 295-306 ◽  
Author(s):  
Nabila Yahiaoui, Christiane Marque ◽  
Hélène Corbière ◽  
Alain Michel Boudet
Keyword(s):  
Alcohol Dehydrogenase ◽  
Cinnamyl Alcohol Dehydrogenase ◽  
Cinnamyl Alcohol ◽  
Down Regulation ◽  
Comparative Efficiency

Isolation and expression of a cinnamyl alcohol dehydrogenase cDNA from perennial ryegrass (Lolium perenne)

2001 ◽  
Vol 28 (11) ◽  
pp. 1085
Author(s):  
Fiona M. McAlister ◽  
Wendy R. Lewis-Henderson ◽  
Colin L. D. Jenkins ◽  
John M. Watson
Keyword(s):  
Alcohol Dehydrogenase ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Blot Hybridization ◽  
Substrate Inhibition ◽  
Cinnamyl Alcohol Dehydrogenase ◽  
Southern Blot Hybridization ◽  
Leaf Sheath ◽  
Cinnamyl Alcohol ◽  
Degenerate Oligonucleotide

A perennial ryegrass (Lolium perenne L.) cDNA library was screened with a PCR-amplified cad DNA fragment generated from ryegrass cDNA template using degenerate oligonucleotide primers. A full-length cDNA (LpeCad1) was isolated and confirmed to encode a cinnamyl alcohol dehydrogenase (CAD) enzyme by expression of activity in Escherichia coli. The recombinant enzyme catalyses conversion of coniferaldehyde and sinapaldehyde with similar efficiency, and apparent K m values below 10 µM were determined for these substrates, whereas weak substrate inhibition occurs above this concentration. The predicted perennial ryegrass CAD was very similar (88–87percnt; amino acid sequence identity) to the only other monocotyledonous plant CAD sequences available, those of maize and sugarcane, respectively. Southern blot hybridization analysis indicated that there may be two or three cad genes, or alleles, in perennial ryegrass. The ryegrass LpeCad1 gene resembles the maize cadgene in showing strong expression in root and stem tissues, but is also expressed at lower levels in shoot, leaf sheath, leaf blade and floral tissues.

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