Comparative investigation of primary and tertiary endodermal cell walls isolated from the roots of five monocotyledoneous species: chemical composition in relation to fine structure

Planta ◽  
1998 ◽  
Vol 206 (3) ◽  
pp. 349-361 ◽  
Author(s):  
Jürgen Zeier ◽  
Lukas Schreiber
Science ◽  
1984 ◽  
Vol 225 (4662) ◽  
pp. 621-623 ◽  
Author(s):  
E. L. SMOOT ◽  
T. N. TAYLOR

2020 ◽  
Author(s):  
Huimin Xu ◽  
Yuanyuan Zhao ◽  
Yuanzhen Suo ◽  
Yayu Guo ◽  
Yi Man ◽  
...  

Abstract Background: Cell wall imaging can considerably permit direct visualization of the molecular architecture of cell walls and provide the detailed chemical information on wall polymers, which is imperative to better exploit and use the biomass polymers; however, detailed imaging and quantifying of the native composition and architecture in the cell wall remains challenging.Results: Here, we describe a label-free imaging technology, coherent Raman scattering microscopy (CRS), including coherent anti-Stokes Raman scattering (CARS) microscopy and stimulated Raman scattering (SRS) microscopy, which images the major structures and chemical composition of plant cell walls. The major steps of the procedure are demonstrated, including sample preparation, setting the mapping parameters, analysis of spectral data, and image generation. Applying this rapid approach, which will help researchers understand the highly heterogeneous structures and organization of plant cell walls.Conclusions: This method can potentially be incorporated into label-free microanalyses of plant cell wall chemical composition based on the in situ vibrations of molecules.


Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
João Alves Ferreira Pereira ◽  
Ítalo Antônio Cotta Coutinho ◽  
Emanoella Lima Soares ◽  
Arlete Aparecida Soares ◽  
Ana Paula de Souza Caetano ◽  
...  

Abstract Although the consumption of açaí (Euterpe oleracea) pulp has long been an important component of the diet of the peoples from the Amazon, the açaí palm tree has recently attracted economic and scientific interest because of its vast array of bioactive compounds found in the fruit pericarp. The açaí seeds are the largest byproduct after pulp extraction and have potential for use in ethanol production, but this process is hindered by limited knowledge of seed biology, chemical composition and pattern reserve deposition during seed development. The aim of this work was to describe the morphoanatomical development of the seeds, as well as to identify the main organic compounds stored in the seeds. To achieve this goal, histological and histochemical analyses were performed on developing seeds. Results showed the seed is albuminous, bitegmic and that ingrowths of the seed coat give rise to a ruminate endosperm. Moreover, the nutritive reserves of açaí seeds are found in the endosperm thickened cell walls as reserve polysaccharides. Our findings provide information for future studies dealing with reproductive biology, propagation and the improvement of this profitable crop.


CONVERSAZIONES were held this year on 9 May and 27 June. At the first conversazione twenty-seven exhibits and two films were shown. The fine structure of plant roots in relation to transport of nutrient ions and water was demonstrated by Dr D. T. Clarkson of the A.R.C. Letcombe Laboratory, Wantage and Dr A. W. Robards of the Department of Biology, University of York. Two major pathways by which nutrients and water move radially across the cortex towards the central vascular tissue have been distinguished by the use of tracer studies of adsorption by different zones of intact root systems, microautoradiography and electron microscopy. Movement can be apoplastic through cell walls, or symplastic between cells joined by plasmodesmata. As the root ages, structural changes in the endodermis reduce movement in the former pathway but the symplast is not interrupted by the elaboration of endodermal walls because plasmodesmatal connexions remain intact. These observations help explain the contrasting extent to which different ions and water reach the shoot from young and mature parts of root systems.


2020 ◽  
Vol 107 ◽  
pp. 105858
Author(s):  
Shiyi Lu ◽  
Bernadine M. Flanagan ◽  
Barbara A. Williams ◽  
Deirdre Mikkelsen ◽  
Michael J. Gidley

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