Light-independent degradation of stromal proteins in intact chloroplasts isolated from Pisum sativum L. leaves: requirement for divalent cations

Planta ◽  
1998 ◽  
Vol 205 (2) ◽  
pp. 297-304 ◽  
Author(s):  
Samuel Roulin ◽  
Urs Feller
Keyword(s):  
Pisum Sativum ◽  
Divalent Cations ◽  
Pisum Sativum L ◽  
Intact Chloroplasts ◽  
Stromal Proteins

Degradation of glutamine synthetase in intact chloroplasts isolated from pea (Pisum sativum) leaves

1998 ◽  
Vol 25 (3) ◽  
pp. 279 ◽  
Author(s):  
Melanie Thoenen ◽  
Urs Feller
Keyword(s):  
Pisum Sativum ◽  
Glutamine Synthetase ◽  
Active Oxygen Species ◽  
Co2 Assimilation ◽  
Methionine Sulfoximine ◽  
General Effect ◽  
Chloroplast Proteins ◽  
Intact Chloroplasts ◽  
The Stability ◽  
Stromal Proteins

Chloroplast proteins can be degraded in the intact organelle, but the relative rates of degradation vary considerably. To investigate regulatory aspects of glutamine synthetase (GS) degradation, isolated pea (Pisum sativum L.) chloroplasts were used as a suitable system. Since chloroplasts were re-isolated after incubation, only chloroplasts remaining intact throughout the incubation and processes occurring therein were analysed. Net changes of nuclear-encoded proteins indicate degradation because protein synthesis is no longer possible under these conditions. Incubation of intact chloroplasts in a medium which promotes CO2 -assimilation led to the stabilisation of GS and several other chloroplast proteins. This general effect suggests that photosynthetic metabolism is relevant for the stability of stromal proteins. In addition, GS was specifically stabilised by methionine sulfoximine (MSO), a potent inhibitor of GS. However, other enzymes tested were not affected by MSO. When chloroplasts were incubated with methyl viologen, the degradation of several stromal enzymes including GS was accelerated, most likely by active oxygen species. Again, MSO specifically delayed the degradation of GS. As MSO is a substrate analogue, it appears likely that substrates or other ligands influence the susceptibility of enzymes against degradation systems present in the same compartment.

scholarly journals An improved procedure for the isolation of intact chloroplasts of high photosynthetic capacity

1984 ◽  
Vol 223 (2) ◽  
pp. 543-545 ◽  
Author(s):  
Z G Cerović ◽  
M Plesnicar
Keyword(s):  
Pisum Sativum ◽  
Photosynthetic Capacity ◽  
Starting Material ◽  
High Reproducibility ◽  
Pisum Sativum L ◽  
High Photosynthetic Capacity ◽  
Intact Chloroplasts ◽  
Mechanical Isolation ◽  
High Degree

An improved procedure for the mechanical isolation of chloroplasts of high degree of intactness (90-95%) and photosynthetic capacity (25-50 mmol of O2/s per mol of chlorophyll) is described. The combination of pea plants (Pisum sativum L.) as starting material and the high reproducibility of the procedure readily and cheaply yields reliable intact chloroplasts for photosynthetic studies.

Mitigation Effects of 24-Epibrassinolide and Thiourea in Field Pea (Pisum sativum L.) under Drought Stress

2018 ◽  
Vol 34 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Prachi Garg ◽  
◽  
A. Hemantaranjan ◽  
Jyostnarani Pradhan ◽  
◽  
...  
Keyword(s):  
Drought Stress ◽  
Pisum Sativum ◽  
Field Pea ◽  
Pisum Sativum L

МЕТОД ЭКСПРЕСС-ОЦЕНКИ АЛЮМОТОЛЕРАНТНОСТИ У ГОРОХА ПОСЕВНОГО (Pisum sativum L.)

2015 ◽  
Vol 50 (3) ◽  
pp. 353-360
Author(s):  
М.А. ВИШНЯКОВА ◽  
◽  
Е.В. СЕМЕНОВА ◽  
И.А. КОСАРЕВА ◽  
Н.Д. КРАВЧУК ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Pisum Sativum L
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