Localization of pectins in the pollen tube wall of Ornithogalum virens L. Does the pattern of pectin distribution depend on the growth rate of the pollen tube?

Planta ◽  
2000 ◽  
Vol 210 (4) ◽  
pp. 630-635 ◽  
Author(s):  
Małgorzata Stępka ◽  
Fabricio Ciampolini ◽  
Maria Charzyńska ◽  
Mauro Cresti
Keyword(s):  
Growth Rate ◽  
Pollen Tube ◽  
Tube Wall ◽  
Pollen Tube Wall ◽  
Ornithogalum Virens

The growth of the pollen tube wall in Oenothera organensis

1975 ◽  
Vol 18 (3) ◽  
pp. 519-532
Author(s):  
H.G. Dickinson ◽  
J. Lawson
Keyword(s):  
Pollen Tube ◽  
Tube Wall ◽  
Wall Material ◽  
Fibrillar Material ◽  
Stage Of Development ◽  
Large Numbers ◽  
Membrane Bound ◽  
Pollen Tube Wall ◽  
Different Sources ◽  
Dictyosome Vesicles

The growth of the pollen tube wall of Oenothera is effected by the expulsion of fibrillar material from the cytoplasm into the developing wall. This material may also be seen in the cytoplasm, contained in membrane-bound vesicles. It is not clear how the content of the vesicles is discharged, but it appears not to involve the participation of microtubules. The source of the cytoplasmic fibrillar bodies depends upon the stage of development of the pollen tube. The earilest growth is derived from the inclusion into the wall of vesicles containing pre-formed materials present in the grain on pollination. During the next stage of growth the wall is derived from the content of double-membraned inclusions also present in the pollen. The content of the former vesicles is not so similar to the wall as the latter, but intermediates between the 2 types of vesicle may be seen in the cytoplasm, indicating that the former are formed from the latter. Most of the tube wall is derived from the products of dictyosomes in the pollen grain or tube. These dicytosomes are few in number and they must be exceedingly active. This, and the observation that dictyosome vesicles are frequently associated with banked complexes of mitochondria, indicates that some steps in the metabolism of the vesicular content, perhaps phosphorylation, take place distant from the dicytosomes. These different sources of fibrillar material presumably permit the rapid starting of tube growth, without any attendant metabolism. However, it would be impossible to include enough pre-formed wall material in the grain to enable the full growth of the tube, so once started, it seems that the tube then relies on the elaboration of simple reserves for the contruction of its wall. These reserves are likely to be held in the pollen, and may be the large numbers of starch grains characteristic of the pollen cytoplasm.

Observations of Germinating Lychnis Alba Pollen

1971 ◽  
Vol 29 ◽  
pp. 348-349
Author(s):  
Richard E. Crang ◽  
Michael A. Millay
Keyword(s):  
Electron Microscopy ◽  
Pollen Tube ◽  
Liquid Culture ◽  
Culture Medium ◽  
Tube Wall ◽  
Pollen Grains ◽  
Pharbitis Nil ◽  
Liquid Culture Medium ◽  
Transmission Electron ◽  
Pollen Tube Wall

The exine surface of Lychnis alba pollen grains is ornamented with spines and pits (Fig. 1) that are variable both in size and number. No relationship appears to exist between the relative nature of these surface features as observed by means of scanning electron microscopy (SEM) and germination potentials of the pollen. The protrusion of cytoplasm at the apertures is a common phenomenon as the grains become hydrated when placed in liquid culture medium. As swelling of the apertures occurs, the aperturate opercula, or pore plates, may be lifted to the terminal surface but frequently are displaced to one side where they become embedded in the pollen tube wall (Fig.2). Although all apertures may protrude, only a single pollen tube will normally form from each grain. The composition of the opercula appear similar to the exine in transmission electron microscopy (TEM) preparations, but is less dense than the exine when observed in SEM preparations, as indicated by surface folds suggestive of a soft composition. There is no structural evidence that enzyme degradations of the exine at germination sites is required for emergence of the pollen tube, although such may be the case when pollen germinates on the style as indicated in SEM observations of Pharbitis nil pollen.

scholarly journals Structural cell wall proteins from five pollen species and their relationship with boron

2005 ◽  
Vol 17 (4) ◽  
pp. 375-381 ◽  
Author(s):  
Edith del R. García-Hernández ◽  
Gladys I. Cassab López
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Tube Wall ◽  
Maize Pollen ◽  
Structural Cell ◽  
Pollen Species ◽  
Dicotyledonous Plants ◽  
Zea May ◽  
Pollen Tube Wall

Boron (B) is an essential micronutrient for the survival of vascular plants. The most severe effect derived from a deficiency of B is the alteration of cell wall biogenesis and pollen germination. We investigated pollen of plant species that require B for germination (Zea may L. and Nicotiana tabacum L.), as well as those that can germinate without B (Pinus sp, Lilum longiflorum, Impatiens sp.). In both groups, B addition in the growth medium increased the length of the pollen tube after germination. Hydroxyproline Rich Glycoproteins (HRGPs) are the most abundant cell wall structural proteins of dicotyledonous plants and the sexual tissues of monocotyledonous plants. Here, we show that maize pollen accumulated a significant pool of hydroxyproline (Hyp) and 63% of this amino acid was localized in the pollen tube wall. Maize pollen germinated in the presence of B accumulated soluble (48%) and non-soluble (16%) Hyp in the pollen tube wall in contrast to maize pollen germinated without B. In addition, B seems to modify the amount of HRGPs that become cross-linked to the wall. Immunolocalization of HRGPs showed that these glycoproteins were preferentially localized in the pollen tube of maize, not in the pollen grain itself. Hence, B might affect the assembling of HRGPs in the wall of pollen tubes grown in vitro.

Glycoproteins 66 and 69 kDa of pollen tube wall: properties and distribution in angiosperms

2001 ◽  
Vol 158 (11) ◽  
pp. 1367-1374 ◽  
Author(s):  
Alena Fidlerová ◽  
Petr Smýkal ◽  
Jaroslav Tupý ◽  
Věra Čapková
Keyword(s):  
Pollen Tube ◽  
Tube Wall ◽  
Wall Properties ◽  
Pollen Tube Wall

Hemicellulose of the pollen tube wall of Camellia japonica

1980 ◽  
Vol 21 (8) ◽  
pp. 1383-1390 ◽  
Author(s):  
N. Nakamura ◽  
K. Yoshida ◽  
H. Suzuki
Keyword(s):  
Pollen Tube ◽  
Tube Wall ◽  
Camellia Japonica ◽  
Pollen Tube Wall
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