Functional characterisation of Nicotiana tabacum xyloglucan endotransglycosylase ( Nt XET-1): generation of transgenic tobacco plants and changes in cell wall xyloglucan

Planta ◽  
2001 ◽  
Vol 212 (2) ◽  
pp. 279-287 ◽  
Author(s):  
Karin Herbers ◽  
Ester P. Lorences ◽  
Coral Barrachina ◽  
Uwe Sonnewald
Keyword(s):  
Cell Wall ◽  
Nicotiana Tabacum ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Xyloglucan Endotransglycosylase ◽  
Tobacco Plants ◽  
Functional Characterisation

scholarly journals Lead Phytoremediation Potential of Wild Type and Transgenic Tobacco Plants

2021 ◽  
Vol 5 (1) ◽  
pp. 168-182
Author(s):  
Hatice DAGHAN ◽  
Veli UYGUR ◽  
Abdullah EREN
Keyword(s):  
Nicotiana Tabacum ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants ◽  
Nicotiana Tabacum L ◽  
Lead Phytoremediation

Genetiği değiştirilmiş bitkiler, kurşunun (Pb) kökten yer üstü kısımlarına translokasyonunu geliştirmek için büyük bir potansiyele sahip olabilir. Transgenik olmayan ( Nicotiana tabacum L. cv. Petit Havana SR1) ve transgenik (p-cV-ChMTII GFP) tütün bitkileri tarafından Pb alımının sağlanması araştırmak için Çin hamsteri metalotiyonin II gezen bir kap deneyi yapıldı . Transgenik ve transgenik olmayan tütün bitkileri, 0, 1000, 2500, 5000 mg Pb kg- 1 ile Pb (NO 3 ) 2 olarak işlenmiş topraklarda yetiştirildi. Kelimede bir büyüme bölümünde 6 hafta boyunca çiçeklenme aşamasına kadar.Bitkilerin büyümesi, klorofil içeriği, mineral besin elementleri ve düşük glutatyon (GSH) bezleri, bitkilerin Pb alım potansiyeli ile birlikte incelenmiştir. Hem transgenik hem de transgenik olmayan bitkiler için Pb uygulamasındaki artışa bağlı olarak yer üstü biyokütle çevrildi aşamalı bir düşüş gözlendi. Yaprak besinlerinin bulaştığı, aşırı Pb işlemlerinden olumsuz etkilenmiştir, bunlardan en büyük düşüşü. Sürgün Pb yüksek derecesi 76.0 mg kg kadar ulaşan -1 transgenik ve 70.9 mg kg -1 transgenik olmayan bitkilerde. Pb alımı, p-cV-ChMTII GFP'nin tütün bitkisine aktarılmasıyla iyileştirildi; ancak, Pb fitoremediasyonunda yeterli değildi. 

Large-scale phenotyping of transgenic tobacco plants (Nicotiana tabacum) to identify essential leaf functions

2008 ◽  
Vol 6 (3) ◽  
pp. 246-263 ◽  
Author(s):  
Wolfgang Lein ◽  
Bjoern Usadel ◽  
Mark Stitt ◽  
Andreas Reindl ◽  
Thomas Ehrhardt ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Transgenic Tobacco ◽  
Large Scale ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants

Reassessment of qualitative changes in lignification of transgenic tobacco plants and their impact on cell wall assembly

2001 ◽  
Vol 57 (6) ◽  
pp. 875-882 ◽  
Author(s):  
Katia Ruel ◽  
Matthieu Chabannes ◽  
Alain-Michel Boudet ◽  
Michel Legrand ◽  
Jean-Paul Joseleau
Keyword(s):  
Cell Wall ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Cell Wall Assembly ◽  
Wall Assembly ◽  
Qualitative Changes

scholarly journals Hevea brasiliensis coniferaldehyde-5-hydroxylase (HbCAld5H) regulates xylogenesis, structure and lignin chemistry of xylem cell wall in Nicotiana tabacum

2020 ◽  
Author(s):  
S. Pramod ◽  
Thakurdas Saha ◽  
K. Rekha ◽  
P. B. Kavi Kishor
Keyword(s):  
Cell Wall ◽  
Transgenic Tobacco ◽  
Hevea Brasiliensis ◽  
Secondary Wall ◽  
Cambial Activity ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Lignin Distribution ◽  
Syringyl Lignin ◽  
S2 Layer

Abstract Key message The HbCAld5H1 gene cloned from Hevea brasiliensis regulates the cambial activity, xylem differentiation, syringyl–guaiacyl ratio, secondary wall structure, lignification pattern and xylan distribution in xylem fibres of transgenic tobacco plants. Abstract Molecular characterization of lignin biosynthesis gene coniferaldehyde-5-hydroxylase (CAld5H) from Hevea brasiliensis and its functional validation was performed. Both sense and antisense constructs of HbCAld5H1 gene were introduced into tobacco through Agrobacterium-mediated genetic transformation for over expression and down-regulation of this key enzyme to understand its role affecting structural and cell wall chemistry. The anatomical studies of transgenic tobacco plants revealed the increase of cambial activity leading to xylogenesis in sense lines and considerable reduction in antisense lines. The ultra-structural studies showed that the thickness of secondary wall (S2 layer) of fibre had been decreased with non-homogenous lignin distribution in antisense lines, while sense lines showed an increase in S2 layer thickness. Maule color reaction revealed that syringyl lignin distribution in the xylem elements was increased in sense and decreased in antisense lines. The immunoelectron microscopy revealed a reduction in LM 10 and LM 11 labelling in the secondary wall of antisense tobacco lines. Biochemical studies showed a radical increase in syringyl lignin in sense lines without any significant change in total lignin content, while S/G ratio decreased considerably in antisense lines. Our results suggest that CAld5H gene plays an important role in xylogenesis stages such as cambial cell division, secondary wall thickness, xylan and syringyl lignin distribution in tobacco. Therefore, CAld5H gene could be considered as a promising target for lignin modification essential for timber quality improvement in rubber.

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