Conditional identification of phosphate-starvation-response mutants in Arabidopsis thaliana

Planta ◽  
2000 ◽  
Vol 211 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Donna L. Chen ◽  
Carla A. Delatorre ◽  
Aleida Bakker ◽  
Steffen Abel
Keyword(s):  
Arabidopsis Thaliana ◽  
Phosphate Starvation ◽  
Starvation Response ◽  
Phosphate Starvation Response

scholarly journals Cloning, Characterization and Expression Analysis of the Phosphate Starvation Response Gene, ClPHR1, from Chinese Fir

Forests ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 104
Author(s):  
Wanting Chen ◽  
Ranhong Chen ◽  
Ying Zhang ◽  
Jiaoyang Li ◽  
Mulualem Tigabu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Phosphate Starvation ◽  
Chinese Fir ◽  
Response Gene ◽  
Starvation Response ◽  
Stress Treatment ◽  
Phosphate Starvation Response ◽  
Low Pi Stress ◽  
Rapid Amplification ◽  
Pi Stress

The study on the function and sequence of PHR1 (Phosphate Starvation Response gene 1) gene, which plays a central role in plant phosphorus (Pi) signal regulatory network, is of great significance to further study response mechanisms to Pi deficiency. In this work, the previously selected Pi-efficient Chinese fir clone M32 was used as research material to obtain the full-length sequence of ClPHR1 transcription factors in Chinese fir by RACE (Rapid Amplification of cDNA Ends) full-length cloning technique, and the structure, function and subcellular localization of ClPHR1 gene encoding protein were analyzed. The temporal and spatial expression characteristics of ClPHR1 transcription factors in Chinese fir under low Pi stress were also analyzed, and the overexpression of ClPHR1 gene in transgenic Arabidopsis thaliana was obtained to verify the function of ClPHR1 gene under low Pi stress. The results showed that the length of the ClPHR1 gene obtained by rapid amplification of cDNA ends technique was 1954 bp, of which 1512 bp was an open reading frame. ClPHR1 was predicted to be an unstable hydrophilic protein with only one possible transmembrane domain. The ClPHR1 gene had a highly conserved MYB-CC domain, which is similar to the PHR1 gene of other plants. Phylogenetic tree analysis showed that the sequence had high homology with PHR1 genes in the Prunus species. The ClPHR1 was expressed in all organs of Chinese fir, with the highest expression in the roots, followed by the leaves with the lowest expression in stems. ClPHR1 expression in roots was reduced dramatically at the beginning of Pi stress treatment and followed by an increase at 7days; in leaves, it increased dramatically at the beginning of Pi starvation treatment and showed a decreasing trend after 3 days; in stems, the expression level of ClPHR1 increased after 7 days of Pi stress treatment. The transient expression vector was introduced into plant cells, and it was found that ClPHR1 was located in the nucleus and was a MYB-CC transcription factor expressed in the cell nucleus. The ClPHR1 overexpression vector was constructed, and then introduced into Arabidopsis thaliana by agrobacterium infection inflorescence method. The expressions of Pi transporter genes, AtPHT1;1, AtPHT1;2, AtPHT1;8 and AtPHT1;9, was significantly higher in the overexpressing strain than that in the wild type strain. The results suggest that the ClPHR1 transcription factor could regulate the regulation of downstream Pi transporter gene and increase Pi utilization efficiency of the Chinese fir under Pi stress.

scholarly journals Differentiating phosphate-dependent and phosphate-independent systemic phosphate-starvation response networks in Arabidopsis thaliana through the application of phosphite

2015 ◽  
Vol 66 (9) ◽  
pp. 2501-2514 ◽  
Author(s):  
Ricarda Jost ◽  
Made Pharmawati ◽  
Hazel R. Lapis-Gaza ◽  
Claudia Rossig ◽  
Oliver Berkowitz ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Phosphate Starvation ◽  
Starvation Response ◽  
Phosphate Starvation Response

scholarly journals Phosphate starvation response controls genes required to synthesize the phosphate analog arsenate

2018 ◽  
Vol 20 (5) ◽  
pp. 1782-1793 ◽  
Author(s):  
Qian Wang ◽  
Yoon-Suk Kang ◽  
Abdullah Alowaifeer ◽  
Kaixiang Shi ◽  
Xia Fan ◽  
...  
Keyword(s):  
Phosphate Starvation ◽  
Starvation Response ◽  
Phosphate Starvation Response

The phosphate-starvation response inVibrio cholerae O1 andphoB mutant under proteomic analysis: Disclosing functions involved in adaptation, survival and virulence

PROTEOMICS ◽  
2006 ◽  
Vol 6 (5) ◽  
pp. 1495-1511 ◽  
Author(s):  
Wanda Maria Almeida von Krüger ◽  
Leticia Miranda Santos Lery ◽  
Marcia Regina Soares ◽  
Fernanda Saloum de Neves-Manta ◽  
Celia Maria Batista e Silva ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Phosphate Starvation ◽  
Starvation Response ◽  
Phosphate Starvation Response
Sign in / Sign up

Export Citation Format

Share Document