Simultaneous multi-site recordings of neural activity with an inline multi-electrode array and optical measurement in rat hippocampal slices

2001 ◽  
Vol 443 (2) ◽  
pp. 317-322 ◽  
Author(s):  
Takashi Tominaga ◽  
Yoko Tominaga ◽  
Michinori Ichikawa
Keyword(s):  
Neural Activity ◽  
Optical Measurement ◽  
Hippocampal Slices ◽  
Electrode Array ◽  
Multi Electrode Array

scholarly journals Characterizing the short-latency evoked response to intracortical microstimulation across a multi-electrode array

2021 ◽  
Author(s):  
Joseph Sombeck ◽  
Juliet Heye ◽  
Karthik Kumaravelu ◽  
Stefan M Goetz ◽  
Angel V Peterchev ◽  
...  
Keyword(s):  
Neural Activity ◽  
Electrode Array ◽  
Evoked Response ◽  
Intracortical Microstimulation ◽  
Visually Guided ◽  
Robotic Arms ◽  
Somatosensory Feedback ◽  
Wide Range ◽  
Evoked Activity ◽  
Multi Electrode Array

Objective: Persons with tetraplegia can use brain-machine interfaces to make visually guided reaches with robotic arms. Without somatosensory feedback, these movements will likely be slow and imprecise, like those of persons who retain movement but have lost proprioception. Intracortical microstimulation (ICMS) has promise for providing artificial somatosensory feedback. If ICMS can mimic naturally occurring neural activity, afferent interfaces may be more informative and easier to learn than interfaces that evoke unnaturalistic activity. To develop such biomimetic stimulation patterns, it is important to characterize the responses of neurons to ICMS. Approach: Using a Utah multi-electrode array, we recorded activity evoked by single pulses, and short (~0.2 s) and long (~4 s) trains of ICMS at a wide range of amplitudes and frequencies. As the electrical artifact caused by ICMS typically prevents recording for many milliseconds, we deployed a custom rapid-recovery amplifier with nonlinear gain to limit signal saturation on the stimulated electrode. Across all electrodes after stimulation, we removed the remaining slow return to baseline with acausal high-pass filtering of time-reversed recordings. With these techniques, we could record ~0.7 ms after stimulation offset even on the stimulated electrode. Main results: We recorded likely transsynaptically-evoked activity as early as ~0.7 ms after single pulses of stimulation that was immediately followed by suppressed neural activity lasting 10-150 ms. Instead of this long-lasting inhibition, neurons increased their firing rates for ~100 ms after trains. During long trains, the evoked response on the stimulated electrode decayed rapidly while the response was maintained on non-stimulated channels. Significance: The detailed description of the spatial and temporal response to ICMS can be used to better interpret results from experiments that probe circuit connectivity or function of cortical areas. These results can also contribute to the design of stimulation patterns to improve afferent interfaces for artificial sensory feedback.

scholarly journals Patch-clamp and multi-electrode array electrophysiological analysis in acute mouse brain slices

2021 ◽  
Vol 2 (2) ◽  
pp. 100442
Author(s):  
Kevin M. Manz ◽  
Justin K. Siemann ◽  
Douglas G. McMahon ◽  
Brad A. Grueter
Keyword(s):  
Patch Clamp ◽  
Mouse Brain ◽  
Brain Slices ◽  
Electrode Array ◽  
Electrophysiological Analysis ◽  
Multi Electrode Array

scholarly journals Multiple states in ongoing neural activity in the rat visual cortex

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0256791
Author(s):  
Daichi Konno ◽  
Shinji Nishimoto ◽  
Takafumi Suzuki ◽  
Yuji Ikegaya ◽  
Nobuyoshi Matsumoto
Keyword(s):  
Visual Cortex ◽  
Neural Activity ◽  
Activity Patterns ◽  
Electrode Array ◽  
Nonlinear Dimensionality Reduction ◽  
Neural Responses ◽  
Spontaneous Neural Activity ◽  
Custom Made ◽  
Visual Cortices ◽  
Freely Behaving

The brain continuously produces internal activity in the absence of afferently salient sensory input. Spontaneous neural activity is intrinsically defined by circuit structures and associated with the mode of information processing and behavioral responses. However, the spatiotemporal dynamics of spontaneous activity in the visual cortices of behaving animals remain almost elusive. Using a custom-made electrode array, we recorded 32-site electrocorticograms in the primary and secondary visual cortex of freely behaving rats and determined the propagation patterns of spontaneous neural activity. Nonlinear dimensionality reduction and unsupervised clustering revealed multiple discrete states of the activity patterns. The activity remained stable in one state and suddenly jumped to another state. The diversity and dynamics of the internally switching cortical states would imply flexibility of neural responses to various external inputs.

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