Action potential propagation failures in long-term recordings from embryonic stem cell-derived cardiomyocytes in tissue culture

1999 ◽  
Vol 437 (5) ◽  
pp. 669-679 ◽  
Author(s):  
Peter Igelmund ◽  
Bernd K. Fleischmann ◽  
Ivo R. Fischer ◽  
Julia Soest ◽  
Oleksii Gryshchenko ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Stem Cell ◽  
Action Potential ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Action Potential Propagation

Optimization of Culture Conditions to Support Long-Term Self-Renewal of Buffalo (Bubalus bubalis) Embryonic Stem Cell-Like Cells

2011 ◽  
Vol 13 (6) ◽  
pp. 539-549 ◽  
Author(s):  
Ruchi Sharma ◽  
Aman George ◽  
Nitin Manchindra Kamble ◽  
Karn Pratap Singh ◽  
Manmohan Singh Chauhan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Bubalus Bubalis ◽  
Culture Conditions ◽  
Self Renewal ◽  
Optimization Of Culture Conditions

scholarly journals Puerarin Enhances Ca2+ Reuptake and Ca2+ Content of Sarcoplasmic Reticulum in Murine Embryonic Stem Cell-Derived Cardiomyocytes via Upregulation of SERCA2a

2017 ◽  
Vol 44 (3) ◽  
pp. 1199-1212
Author(s):  
Lu Wang ◽  
Yurong Cui ◽  
Qinghua Liu ◽  
Yuanlong Song ◽  
Qinghua Hu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Sarcoplasmic Reticulum ◽  
Protein Expression ◽  
Embryonic Stem Cell ◽  
Embryoid Body ◽  
Embryonic Stem ◽  
Calcium Transient ◽  
Cardiac Differentiation ◽  
Patch Clamps

Background/Aims: The embryonic stem cell-derived cardiomyocytes (ES-CMs) serve as potential sources for cardiac regenerative therapy. However, the immature sarcoplasmic reticulum (SR) function of ES-CMs prevents its application. In this report, we examined the effect of puerarin, an isoflavone compound, on SR function of murine ES-CMs. Methods: Murine ES-CMs were harvested by embryoid body-based differentiation method. Confocal calcium imaging and whole-cell patch clamps were performed to assess the function of SR. The mRNA expression levels of SR-related genes were examined by quantitative PCR. The protein expression of sarcoplasmic reticulum calcium-ATPase 2a (SERCA2a) was evaluated by immunofluorescent and western blot. Results: Long-term application of puerarin promotes basic properties of spontaneous calcium transient with increased amplitude, decay velocity, and decreased duration. Puerarin fails to alter ICa,L but increases the Ca2+ content of SR. Puerarin-treated ES-CMs have intact SR Ca2+ cycling with more SR Ca2+ reuptake. Long-term application of puerarin asynchronously upregulates the mRNA and protein expression of SERCA2a, as well as the transcripts of calsequestrin and triadin in developing ES-CMs. Application of puerarin during the stage of post-cardiac differentiation upregulates dose-dependently the transcripts of SERCA2a, phospholamban and tridin which can be reversed by the inhibitors of the PI3K/Akt and MAPK/ERK signaling pathways, but shows no effect on the protein expression of SERCA2a. Conclusion: This study demonstrates that long-term puerarin treatment enhances Ca2+ reuptake and Ca2+ content via upregulation of SERCA2a.

scholarly journals Effects of Long-Term Culture on Human Embryonic Stem Cell Aging

2011 ◽  
Vol 20 (1) ◽  
pp. 127-138 ◽  
Author(s):  
Xiaoyan Xie ◽  
Asimina Hiona ◽  
Andrew Stephen Lee ◽  
Feng Cao ◽  
Mei Huang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Cell Aging ◽  
Long Term Culture ◽  
Stem Cell Aging

Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage

2006 ◽  
Vol 345 (3) ◽  
pp. 926-932 ◽  
Author(s):  
Hirofumi Suemori ◽  
Kentaro Yasuchika ◽  
Kouichi Hasegawa ◽  
Tsuyoshi Fujioka ◽  
Norihiro Tsuneyoshi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Lines ◽  
Term Maintenance
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