Estimation of myofibrillar responsiveness to Ca 2+ in isolated rat ventricular myocytes

1998 ◽  
Vol 436 (5) ◽  
pp. 639-645 ◽  
Author(s):  
K. Hongo ◽  
Yoichiro Kusakari ◽  
Masato Konishi ◽  
Satoshi Kurihara ◽  
Seibu Mochizuki
Keyword(s):  
Ventricular Myocytes ◽  
Rat Ventricular Myocytes ◽  
Isolated Rat

Effect of partial blockade of the Na+/Ca2+-exchanger on Ca2+ handling in isolated rat ventricular myocytes

2007 ◽  
Vol 576 (1-3) ◽  
pp. 1-6 ◽  
Author(s):  
Károly Acsai ◽  
Attila Kun ◽  
Attila S Farkas ◽  
Ferenc Fülöp ◽  
Norbert Nagy ◽  
...  
Keyword(s):  
Ventricular Myocytes ◽  
Rat Ventricular Myocytes ◽  
Partial Blockade ◽  
Isolated Rat

Osmotic changes and transsarcolemmal ion transport during total ischaemia of isolated rat ventricular myocytes

1993 ◽  
Vol 88 (5) ◽  
pp. 396-410 ◽  
Author(s):  
J. W. T. Fiolet ◽  
C. A. Schumacher ◽  
A. Baartscheer ◽  
R. Coronel
Keyword(s):  
Ion Transport ◽  
Ventricular Myocytes ◽  
Rat Ventricular Myocytes ◽  
Isolated Rat

Antagonistic Actions of Halothane and Sevoflurane on Spontaneous Ca2+Release in Rat Ventricular Myocytes

2006 ◽  
Vol 105 (1) ◽  
pp. 58-64 ◽  
Author(s):  
Mark D. Graham ◽  
Philip M. Hopkins ◽  
Simon M. Harrison
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Positive Inotropic Effect ◽  
Ventricular Myocytes ◽  
Inotropic Effect ◽  
Release Process ◽  
Rat Ventricular Myocytes ◽  
Fura 2 ◽  
Threefold Increase ◽  
Subcellular Target ◽  
Isolated Rat

Background Halothane has been reported to sensitize Ca(2+) release from the sarcoplasmic reticulum (SR), which is thought to contribute to its initial positive inotropic effect. However, little is known about whether isoflurane or sevoflurane affect the SR Ca(2+) release process, which may contribute to the inotropic profile of these anesthetics. Methods Mild Ca(2+) overload was induced in isolated rat ventricular myocytes by increase of extracellular Ca(2+) to 2 mM. The resultant Ca(2+) transients due to spontaneous Ca(2+) release from the SR were detected optically (fura-2). Cells were exposed to 0.6 mM anesthetic for a period of 4 min, and the frequency and amplitude of spontaneous Ca(2+) transients were measured. Results Halothane caused a temporary threefold increase in frequency and decreased the amplitude (to 54% of control) of spontaneous Ca(2+) transients. Removal of halothane inhibited spontaneous Ca release before it returned to control. In contrast, sevoflurane initially inhibited frequency of Ca(2+) release (to 10% of control), whereas its removal induced a burst of spontaneous Ca(2+) release. Isoflurane had no significant effect on either frequency or amplitude of spontaneous Ca(2+) release on application or removal. Sevoflurane was able to ameliorate the effects of halothane on the frequency and amplitude of spontaneous Ca(2+) release both on application and wash-off. Conclusions Application of halothane and removal of sevoflurane sensitize the SR Ca(2+) release process (and vice versa on removal). Sevoflurane reversed the effects of halothane, suggesting they may act at the same subcellular target on the SR.

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