Suppression of the carbachol-activated nonselective cationic current by antibody against alpha subunit of G o protein in guinea-pig gastric myocytes

1998 ◽  
Vol 436 (3) ◽  
pp. 494-496 ◽  
Author(s):  
Young Chul Kim ◽  
Sung Joon Kim ◽  
Jae Hoon Sim ◽  
Chin Ho Cho ◽  
Yong-Sung Juhnn ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Alpha Subunit ◽  
Cationic Current ◽  
Gastric Myocytes ◽  
Nonselective Cationic Current

Protein kinase C mediates the desensitization of CCh-activated nonselective cationic current in guinea-pig gastric myocytes

1998 ◽  
Vol 436 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Young Chul Kim ◽  
Sung Joon Kim ◽  
Jae Hoon Sim ◽  
Jae Yeoul Jun ◽  
Tong Mook Kang ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Guinea Pig ◽  
Cationic Current ◽  
Kinase C ◽  
Gastric Myocytes ◽  
Nonselective Cationic Current

scholarly journals Inhibition by Clonidine of the Carbachol-Induced Tension Development and Nonselective Cationic Current in Guinea Pig Ileal Myocytes

2001 ◽  
Vol 87 (2) ◽  
pp. 125-133 ◽  
Author(s):  
SeungSoo Chung ◽  
SeongChun Kwon ◽  
YunSuk Kim ◽  
DuckSun Ahn ◽  
YoungHo Lee ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Tension Development ◽  
Cationic Current ◽  
Nonselective Cationic Current

scholarly journals The effect of C-type natriuretic peptide on delayed rectifier potassium currents in gastric antral circular myocytes of the guinea-pig

2008 ◽  
pp. 55-62
Author(s):  
HY Xu ◽  
X Huang ◽  
M Yang ◽  
J-B Sun ◽  
L-H Piao ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Phosphodiesterase Inhibitor ◽  
Potassium Currents ◽  
Inhibitory Effect ◽  
Delayed Rectifier ◽  
Dependent Manner ◽  
Patch Clamp Technique ◽  
Whole Cell Patch Clamp ◽  
Dependent Protein ◽  
Gastric Myocytes

C-type natriuretic peptides (CNP) play an inhibitory role in smooth muscle motility of the gastrointestinal tract, but the effect of CNP on delayed rectifier potassium currents is still unclear. This study was designed to investigate the effect of CNP on delayed rectifier potassium currents and its mechanism by using conventional whole-cell patch-clamp technique in guinea-pig gastric myocytes isolated by collagenase. CNP significantly inhibited delayed rectifier potassium currents [I(K (V))] in dose-dependent manner, and CNP inhibited the peak current elicited by depolarized step pulse to 86.1+/-1.6 % (n=7, P<0.05), 78.4+/-2.6 % (n=10, P<0.01) and 67.7+/-2.3 % (n=14, P<0.01), at concentrations of 0.01 micromol/l, 0.1 micromol/l and 1 micromol/l, respectively, at +60 mV. When the cells were preincubated with 0.1 micromol/l LY83583, a guanylate cyclase inhibitor, the 1 ?micromol/l CNP-induced inhibition of I(K (V)) was significantly impaired but when the cells were preincubated with 0.1 micromol/l zaprinast, a cGMP-sensitive phosphodiesterase inhibitor, the 0.01 micromol/l CNP-induced inhibition of I(K (V)) was significantly potentiated. 8-Br-cGMP, a membrane permeable cGMP analogue mimicked inhibitory effect of CNP on I(K (V)). CNP-induced inhibition of I(K (V)) was completely blocked by KT5823, an inhibitor of cGMP-dependent protein kinase (PKG). The results suggest that CNP inhibits the delayed rectifier potassium currents via cGMP-PKG signal pathway in the gastric antral circular myocytes of the guinea-pig.

ATP-sensitive K+ current and its modulation by substance P in gastric myocytes isolated from guinea pig

1998 ◽  
Vol 358 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Jae Yeoul Jun ◽  
Cheol Ho Yeum ◽  
Pyung Jin Yoon ◽  
In Youb Chang ◽  
Sung Joon Kim ◽  
...  
Keyword(s):  
Substance P ◽  
Guinea Pig ◽  
K Current ◽  
Gastric Myocytes
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