Cystic fibrosis and non-cystic-fibrosis human nasal epithelium show analogous Na + absorption and reversible block by phenamil

1997 ◽  
Vol 434 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Ulrike Blank ◽  
Claudia Rückes ◽  
Wolfgang Clauss ◽  
Thomas Hofmann ◽  
Hermann Lindemann ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Nasal Epithelium ◽  
Human Nasal Epithelium

Nitric oxide has no beneficial effects on ion transport defects in cystic fibrosis human nasal epithelium

2000 ◽  
Vol 441 (1) ◽  
pp. 133-137 ◽  
Author(s):  
Claudia Rückes-Nilges ◽  
H. Lindemann ◽  
Thorsten Klimek ◽  
Hiltrud Glanz ◽  
Wolf-Michael Weber
Keyword(s):  
Nitric Oxide ◽  
Cystic Fibrosis ◽  
Ion Transport ◽  
Nasal Epithelium ◽  
Beneficial Effects ◽  
Human Nasal Epithelium

scholarly journals Assessing the residual CFTR gene expression in human nasal epithelium cells bearing CFTR splicing mutations causing cystic fibrosis

2013 ◽  
Vol 22 (6) ◽  
pp. 784-791 ◽  
Author(s):  
Laia Masvidal ◽  
Susana Igreja ◽  
Maria D Ramos ◽  
Antoni Alvarez ◽  
Javier de Gracia ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cystic Fibrosis ◽  
Cftr Gene ◽  
Nasal Epithelium ◽  
Human Nasal Epithelium ◽  
Splicing Mutations ◽  
Epithelium Cells

Minor Role of Cl– Secretion in Non-Cystic Fibrosis and Cystic Fibrosis Human Nasal Epithelium

1999 ◽  
Vol 9 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Claudia Rückes-Nilges ◽  
Ulrike Weber ◽  
Hermann Lindemann ◽  
Gerd Münker ◽  
Wolfgang Clauss ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Nasal Epithelium ◽  
Minor Role ◽  
Human Nasal Epithelium

scholarly journals Apical CFTR Expression in Human Nasal Epithelium Correlates with Lung Disease in Cystic Fibrosis

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e57617 ◽  
Author(s):  
Marit Arianne van Meegen ◽  
Suzanne Willemina Julia Terheggen-Lagro ◽  
Kirsten Judith Koymans ◽  
Cornelis Korstiaan van der Ent ◽  
Jeffrey Matthijn Beekman
Keyword(s):  
Cystic Fibrosis ◽  
Lung Disease ◽  
Nasal Epithelium ◽  
Human Nasal Epithelium

scholarly journals Is a diluted seawater-based solution safe and effective on human nasal epithelium?

2021 ◽  
Author(s):  
Song Huang ◽  
Samuel Constant ◽  
Barbara De Servi ◽  
Marisa Meloni ◽  
Amina Saaid ◽  
...  
Keyword(s):  
Wound Repair ◽  
Wound Closure ◽  
Isotonic Saline ◽  
Nasal Epithelium ◽  
Mucin Secretion ◽  
Nasal Irrigation ◽  
Tissue Integrity ◽  
Human Nasal Epithelium ◽  
Pre Treatment

Abstract Purpose Nasal irrigation is an effective method for alleviating several nasal symptoms and regular seawater-based nasal irrigation is useful for maintaining nasal hygiene which is essential for appropriate functioning of the nose and for preventing airborne particles including some pollutants, pathogens, and allergens from moving further in the respiratory system. However, safety studies on seawater-based nasal irrigation are scarce. In this study, the safety and efficacy of a diluted isotonic seawater solution (Stérimar Nasal Hygiene, SNH) in maintaining nasal homeostasis were evaluated in vitro. Methods Safety was assessed by measuring tissue integrity via transepithelial electrical resistance (TEER). Efficacy was measured by mucociliary clearance (MCC), mucin secretion, and tissue re-epithelization (wound repair) assays. All assays were performed using a 3D reconstituted human nasal epithelium model. Results In SNH-treated tissues, TEER values were statistically significantly lower than the untreated tissues; however, the values were above the tissue integrity limit. SNH treatment significantly increased MCC (88 vs. 36 µm/s, p < 0.001) and mucin secretion (1717 vs. 1280 µg/ml, p < 0.001) as compared to untreated cultures. Faster wound closure profile was noted upon pre-SNH treatment as compared to classical isotonic saline solution pre-treatment (90.5 vs. 50.7% wound closure 22 h after wound generation). Conclusion SNH did not compromise the integrity of the nasal epithelium in vitro. Furthermore, SNH was effective for removal of foreign particles through MCC increase and for enhancing wound repair on nasal mucosa.

Shunt resistance and ion permeabilities in normal and cystic fibrosis airway epithelia

1989 ◽  
Vol 256 (5) ◽  
pp. C1054-C1063 ◽  
Author(s):  
N. J. Willumsen ◽  
R. C. Boucher
Keyword(s):  
Cystic Fibrosis ◽  
Steady State ◽  
Apical Membrane ◽  
Short Circuit ◽  
Strongly Correlated ◽  
Shunt Resistance ◽  
Human Nasal Epithelium ◽  
Cystic Fibrosis Airway ◽  
Intracellular Ion Activities ◽  
Ion Activities

A method for determination of shunt resistance (Rs) and absolute conductive ion permeabilities of the apical membrane in epithelia from steady-state data is described. The method assumes that the currents are satisfactorily described by the Goldman-Hodgkin-Katz regime. Its application requires measurements of standard transepithelial electrophysiological parameters and of one or more intracellular ion activities. It is applicable under both open- and short-circuit conditions. The method was tested in an electrophysiological analysis of cultured normal and cystic fibrosis (CF) human nasal epithelium. In 15 normal and 10 CF preparations with mean transepithelial resistances of 338 and 427 omega.cm2, Rs was 412 and 623 omega.cm2, respectively. The Rs values determined with the present method were strongly correlated (r = 0.94) with those obtained with another method available in the electrophysiological literature but were as a mean 20% lower. Amiloride increased Rs by 25% in CF and by 8% in normal preparations. In normal preparations, the apical Cl permeability (PCla) was 3.6 x 10(-6) cm/s, and the apical Na permeability (PNaa) was 1.6 x 10(-6) cm/s. In CF preparations, PCla was reduced to a maximum of 2.3 x 10(-7) cm/s, whereas PNaa was increased to 6.2 x 10(-6) cm/s. The apical membrane electromotive force was -1 mV in normal and 43 mV in CF preparations. It is concluded that the method can be used to calculate Rs, apical membrane ion permeabilities, and electromotive forces from steady-state electrophysiological data.

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