Ca2+ regulated K+ and non-selective cation channels in the basolateral membrane of rat colonic crypt base cells

1996 ◽  
Vol 432 (6) ◽  
pp. 1011-1022 ◽  
Author(s):  
M. Bleich ◽  
N. Riedemann ◽  
R. Warth ◽  
D. Kerstan ◽  
J. Leipziger ◽  
...  
Keyword(s):  
Basolateral Membrane ◽  
Cation Channels ◽  
Colonic Crypt ◽  
Rat Colonic Crypt ◽  
Crypt Base

The cAMP-regulated and 293B-inhibited K+ conductance of rat colonic crypt base cells

1996 ◽  
Vol 432 (1) ◽  
pp. 81-88 ◽  
Author(s):  
R. Warth ◽  
N. Riedemann ◽  
M. Bleich ◽  
W. Van Driessche ◽  
A. E. Busch ◽  
...  
Keyword(s):  
Colonic Crypt ◽  
Rat Colonic Crypt ◽  
Crypt Base

Apical Na+/H+ exchange near the base of mouse colonic crypts

2002 ◽  
Vol 283 (1) ◽  
pp. C358-C372 ◽  
Author(s):  
Jingsong Chu ◽  
Shaoyou Chu ◽  
Marshall H. Montrose
Keyword(s):  
Epithelial Cells ◽  
Lucifer Yellow ◽  
Extracellular Fluid ◽  
Colonic Crypt ◽  
Fluid Flux ◽  
Functional Attributes ◽  
Colonic Crypts ◽  
Vectorial Transport ◽  
Crypt Base ◽  
Exchange Activity

Colonic crypts can absorb fluid, but the identity of the absorptive transporters remains speculative. Near the crypt base, the epithelial cells responsible for vectorial transport are relatively undifferentiated and often presumed to mediate only Cl−secretion. We have applied confocal microscopy in combination with an extracellular fluid marker [Lucifer yellow (LY)] or a pH-sensitive dye (2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein) to study mouse colonic crypt epithelial cells directly adjacent to the crypt base within an intact mucosal sheet. Measurements of intracellular pH report activation of colonocyte Na+/H+ exchange in response to luminal or serosal Na+. Studies with LY demonstrate the presence of a paracellular fluid flux, but luminal Na+ does not activate Na+/H+ exchange in the nonepithelial cells of the lamina propria, and studies with LY suggest that the fluid bathing colonocyte basolateral membranes is rapidly refreshed by serosal perfusates. The apical Na+/H+ exchange in crypt colonocytes is inhibited equivalently by luminal 20 μM ethylisopropylamiloride and 20 μM HOE-694 but is not inhibited by luminal 20 μM S-1611. Immunostaining reveals the presence of epitopes from NHE1 and NHE2, but not NHE3, in epithelial cells near the base of colonic crypts. Comparison of apical Na+/H+exchange activity in the presence of Cl− with that in the absence of Cl− (substitution by gluconate or nitrate) revealed no evidence of the Cl−-dependent Na+/H+ exchange that had been previously reported as the sole apical Na+/H+ exchange activity in the colonic crypt. Results suggest the presence of an apical Na+/H+ exchanger near the base of crypts with functional attributes similar to those of the cloned NHE2 isoform.

scholarly journals ISOLATION OF APICAL MEMBRANES FROM HORMONE-SENSITIVE RAT COLONIC CRYPT CELLS

1989 ◽  
Vol 26 (3) ◽  
pp. 280-280
Author(s):  
S Walther ◽  
P Heinz-Erian ◽  
A Roscher ◽  
Y Shin-Zoulek ◽  
W Stolz ◽  
...  
Keyword(s):  
Colonic Crypt ◽  
Hormone Sensitive ◽  
Apical Membranes ◽  
Rat Colonic Crypt ◽  
Crypt Cells

The basolateral Ca 2+ -dependent K + channel in rat colonic crypt cells

1997 ◽  
Vol 435 (2) ◽  
pp. 267-272 ◽  
Author(s):  
M. S. Nielsen ◽  
R. Warth ◽  
M. Bleich ◽  
B. Weyand ◽  
R. Greger
Keyword(s):  
K Channel ◽  
Colonic Crypt ◽  
Rat Colonic Crypt ◽  
Crypt Cells

scholarly journals Expression of a marker for colonic crypt base cells is correlated with poor prognosis in human colorectal cancer

Gut ◽  
1998 ◽  
Vol 42 (1) ◽  
pp. 63-70 ◽  
Author(s):  
A A M van der Wurff ◽  
J ten Kate ◽  
P T J Marx ◽  
E P M van der Linden ◽  
C C L Beek ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Poor Prognosis ◽  
Colonic Mucosa ◽  
Tumour Progression ◽  
Colonic Crypt ◽  
Human Colorectal Cancer ◽  
Colon Crypt ◽  
Differentiated Cells ◽  
Crypt Base ◽  
Worse Prognosis

Background—There is a need for markers in colorectal cancer which will allow subclassification of stage groups into subgroups with high versus low risk of recurrent disease.Aims—To develop monoclonal antibodies that recognise antigens on immature crypt base cells, on the assumption that in a neoplasm undifferentiated but not the terminally differentiated cells will be responsible for tumour progression.Methods—Colon crypt cells which were isolated from human colonic mucosa by EDTA/EGTA incubation were studied. By stepwise harvesting, crypt base cell enriched fractions were obtained, and after incubation with antibodies against dominant antigens, used as immunogens.Results—Of one crypt base cell specific antibody (5E9), the reactive epitope appeared to be a non-terminal carbohydrate in the mucin O-glycans of the colon. The epitope did not seem to be colon specific, but was expressed in a variety of other tissues. In colorectal carcinomas, 5E9 immunoreactivity identified a subgroup of patients with a tendency for worse prognosis.Conclusion—A mucin associated maturation epitope was identified in colonic crypt base cells, the expression of which in Dukes’ stage B3 colorectal carcinoma may be associated with poor prognosis.

scholarly journals Identification of a cKit+ Colonic Crypt Base Secretory Cell That Supports Lgr5+ Stem Cells in Mice

2012 ◽  
Vol 142 (5) ◽  
pp. 1195-1205.e6 ◽  
Author(s):  
Michael E. Rothenberg ◽  
Ysbrand Nusse ◽  
Tomer Kalisky ◽  
John J. Lee ◽  
Piero Dalerba ◽  
...  
Keyword(s):  
Stem Cells ◽  
Secretory Cell ◽  
Colonic Crypt ◽  
Crypt Base
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