The effects of equinatoxin II on respiration – possible mechanism of the toxin lethality

2000 ◽  
Vol 439 (S1) ◽  
pp. r129-r130 ◽  
Author(s):  
Matjaž Bunc ◽  
Robert Frangež ◽  
Janez Rozman ◽  
Dušan Šuput
Keyword(s):  
Equinatoxin Ii

scholarly journals Mechanisms of Equinatoxin II-Induced Transport through the Membrane of a Giant Phospholipid Vesicle

2002 ◽  
Vol 83 (2) ◽  
pp. 944-953 ◽  
Author(s):  
M. Mally ◽  
J. Majhenc ◽  
S. Svetina ◽  
B. Žekš
Keyword(s):  
Phospholipid Vesicle ◽  
Equinatoxin Ii

scholarly journals Membrane insertion of the N-terminal α-helix of equinatoxin II, a sea anemone cytolytic toxin

2004 ◽  
Vol 384 (2) ◽  
pp. 421-428 ◽  
Author(s):  
Ion GUTIÉRREZ-AGUIRRE ◽  
Ariana BARLIČ ◽  
Zdravko PODLESEK ◽  
Peter MAČEK ◽  
Gregor ANDERLUH ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Lipid Membrane ◽  
Model Systems ◽  
Tryptophan Residue ◽  
Sea Anemones ◽  
Fluorescence Measurements ◽  
Tryptophan Residues ◽  
Equinatoxin Ii ◽  
Α Helix ◽  
Pore Forming Proteins

Equinatoxin II (Eqt-II) is a member of the actinoporins, a unique family of cytotoxins comprising 20 kDa pore-forming proteins isolated from sea anemones. Actinoporins bind preferentially to lipid membranes containing sphingomyelin, and create cation-selective pores by oligomerization of three to four monomers. Previous studies have shown that regions of Eqt-II crucial for its cytolytic mechanism are an exposed aromatic cluster and the N-terminal region containing an amphipathic α-helix. In the present study, we have investigated the transfer of the N-terminal α-helix into the lipid membrane by the use of three mutants containing an additional tryptophan residue in different positions within the amphipathic α-helix (Ile18→Trp, Val22→Trp and Ala25→Trp). The interaction of the mutants with different model systems, such as lipid monolayers, erythrocytes and ghost membranes, was extensively characterized. Intrinsic fluorescence measurements and the use of vesicles containing brominated phospholipids indicated a deep localization of the N-terminal amphipathic helix in the lipid bilayer, except for the case of Val22→Trp. This mutant is stabilized in a state immediately prior to final pore formation. The introduction of additional tryptophan residues in the sequence of Eqt-II has proved to be a suitable approach to monitor the new environments that surround defined regions of the molecule upon membrane interaction.

Lysine 77 is a Key Residue in Aggregation of Equinatoxin II, a Pore-forming Toxin from Sea Anemone Actinia equina

2000 ◽  
Vol 173 (1) ◽  
pp. 47-55 ◽  
Author(s):  
G. Anderluh ◽  
A. Barlič ◽  
C. Potrich ◽  
P. Maček ◽  
G. Menestrina
Keyword(s):  
Sea Anemone ◽  
Equinatoxin Ii

scholarly journals Effects of the Eukaryotic Pore-Forming Cytolysin Equinatoxin II on Lipid Membranes and the Role of Sphingomyelin

2003 ◽  
Vol 84 (4) ◽  
pp. 2382-2392 ◽  
Author(s):  
Boyan B. Bonev ◽  
Yuen-Han Lam ◽  
Gregor Anderluh ◽  
Anthony Watts ◽  
Raymond S. Norton ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Equinatoxin Ii

Structure and Activity of the N-Terminal Region of the Eukaryotic Cytolysin Equinatoxin II†

Biochemistry ◽  
2006 ◽  
Vol 45 (6) ◽  
pp. 1818-1828 ◽  
Author(s):  
Alison Drechsler ◽  
Cristina Potrich ◽  
Jennifer K. Sabo ◽  
Mattia Frisanco ◽  
Graziano Guella ◽  
...  
Keyword(s):  
Terminal Region ◽  
Equinatoxin Ii ◽  
Structure And Activity

Ca2+ and Na+ contribute to the swelling of differentiated neuroblastoma cells induced by equinatoxin-II

Toxicon ◽  
2000 ◽  
Vol 38 (11) ◽  
pp. 1547-1560 ◽  
Author(s):  
Frédéric A. Meunier ◽  
Robert Frangež ◽  
Evelyne Benoit ◽  
Gilles Ouanounou ◽  
Béatrice Rouzaire-Dubois ◽  
...  
Keyword(s):  
Neuroblastoma Cells ◽  
Equinatoxin Ii

pH and Temperature-Induced Molten Globule-Like Denatured States of Equinatoxin II:  A Study by UV-Melting, DSC, Far- and Near-UV CD Spectroscopy, and ANS Fluorescence†

Biochemistry ◽  
1997 ◽  
Vol 36 (47) ◽  
pp. 14345-14352 ◽  
Author(s):  
Nataša Poklar ◽  
Jurij Lah ◽  
Mateja Salobir ◽  
Peter Maček ◽  
Gorazd Vesnaver
Keyword(s):  
Molten Globule ◽  
Cd Spectroscopy ◽  
Equinatoxin Ii ◽  
Denatured States ◽  
Uv Melting
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