Biomechanical properties and a kinetic simulation model of the smooth muscle I2 in the buccal mass of Aplysia

1999 ◽  
Vol 81 (5-6) ◽  
pp. 505-513 ◽  
Author(s):  
Sung-Nien Yu ◽  
Patrick E. Crago ◽  
Hillel J. Chiel
Keyword(s):  
Smooth Muscle ◽  
Simulation Model ◽  
Biomechanical Properties ◽  
Kinetic Simulation ◽  
Buccal Mass

scholarly journals A Kinetic Simulation Model That Describes Catalysis and Regulation in Nitric-oxide Synthase

2000 ◽  
Vol 276 (2) ◽  
pp. 1233-1243 ◽  
Author(s):  
Jérôme Santolini ◽  
Subrata Adak ◽  
Christine M. L. Curran ◽  
Dennis J. Stuehr
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Simulation Model ◽  
Kinetic Simulation ◽  
Oxide Synthase

Yersinia enterocolitica enteritis affects rabbit intestinal longitudinal smooth muscle function

1992 ◽  
Vol 262 (2) ◽  
pp. G278-G284 ◽  
Author(s):  
R. B. Scott ◽  
D. T. Tan
Keyword(s):  
Smooth Muscle ◽  
Weight Gain ◽  
Food Intake ◽  
Yersinia Enterocolitica ◽  
Biomechanical Properties ◽  
Isometric Tension ◽  
Infected Group ◽  
Tension Development ◽  
Longitudinal Smooth Muscle ◽  
Tension Curves

To determine whether Yersinia enterocolitica (YE) enteritis has an effect on the biomechanical properties of intestinal smooth muscle, New Zealand White rabbits (600-900 g) were divided into an infected group (n = 9) and sham-infected animals fed ad libitum (n = 9), or pair fed with the infected group (n = 9). Animals were inoculated with 10(10) organisms of YE in 10 ml NaHCO3 (infected group) or 10 ml NaHCO3 (sham-infected control and pair-fed groups) at time 0. Daily food intake, weight gain, and YE excretion were noted. Six days later animals were killed and longitudinal smooth muscle strips prepared from proximal (P), medial (M), and distal (D) segments of intestine in each treatment group. Isometric tension was recorded in tissue baths perfused with oxygenated Krebs solution and 10(-6) M tetrodotoxin. Basal and active (the response to 10(-5) M carbachol) length-tension curves were generated. Then, with the muscle strips stretched to their optimum length for tension development, the dose response to carbachol and to graded depolarization with KCl was determined. Infected animals had a significantly reduced food intake and weight gain compared with controls. The development of basal tension with stretch was not significantly different in infected compared with control or pair-fed tissues from the same site.(ABSTRACT TRUNCATED AT 250 WORDS)

Kinetic simulation model of protein secretion and accumulation in the cell microcapsules

2008 ◽  
Vol 10 (5) ◽  
pp. 575-582 ◽  
Author(s):  
J. Wikström ◽  
H. Syväjärvi ◽  
A. Urtti ◽  
M. Yliperttula
Keyword(s):  
Simulation Model ◽  
Protein Secretion ◽  
Kinetic Simulation

Kinetic simulation model for steam pyrolysis of shale oil feedstock

1990 ◽  
Vol 29 (4) ◽  
pp. 527-534 ◽  
Author(s):  
Hamid R. Kavianian ◽  
Victor F. Yesavage ◽  
Phillip F. Dickson ◽  
Robert W. Peters
Keyword(s):  
Simulation Model ◽  
Shale Oil ◽  
Kinetic Simulation

Abstract 276: Tissue Engineering a Small Diameter Vascular Graft

2012 ◽  
Vol 32 (suppl_1) ◽  
Author(s):  
Mark R Tatterton ◽  
Stacy-Paul Wilshaw ◽  
Eileen Ingham ◽  
Shervanthi Homer-Vanniasinkam
Keyword(s):  
Smooth Muscle ◽  
Cell Adhesion ◽  
Smooth Muscle Cells ◽  
Cell Lines ◽  
Carotid Arteries ◽  
Small Diameter ◽  
Biomechanical Properties ◽  
Vascular Cells ◽  
Vascular Cell ◽  
Native Artery

Introduction The patency rate of synthetic small diameter grafts for coronary and peripheral arterial bypass remains poor. The aim of this study was to engineer an acellular, biocompatible conduit with biomechanical properties similar to native artery and investigate the regenerative capacity of the conduit using ovine vascular cells. Methods Porcine carotid arteries were harvested and decellularized using an established protocol. In short, arteries were treated sequentially with EDTA, hypotonic buffer, 0.1% (w/v) sodium dodecyl sulphate (SDS), DNase and RNase, hypertonic buffer and sterilised using 0.1% (v/v) peracetic acid. In order to determine the biomechanical properties of the acellular arteries, suture retention strength, ultimate tensile strength and burst pressure measurements were performed in comparison to native tissue. Ovine endothelial cells were harvested from the femoral arteries of sheep following digestion with collagenase. Ovine smooth muscle cells were isolated from ovine arterial explant cultures. The phenotype of the cells was confirmed using antibodies to ovine vascular cell markers (myosin, α-actin, VWF, CD62) by indirect immunofluorescence.. Biocompatability of the scaffolds was assessed using contact and extract cytotoxicity tests with two cell lines (BHK and 3T3) and primary ovine vascular cells (endothelial and smooth muscle cells). The capacity of the acellular arteries to support vascular cell adhesion was determined by seeding cells onto the scaffolds and evaluation using scanning electron microscopy (SEM).. Results Histiological characterization and DNA quantification confirmed successful decellularization of porcine carotid arteries. Decellularized vessels were shown to retain the biomechanical strength and properties of native vessels. Decellularized arteries displayed no evidence of cytotoxicity to cell lines or primary vascular cells. Initial cell adhesion studies confirmed successful adherence of ovine vascular cells to decellularized arteries. Conclusion The study resulted in an acellular, biocompatible scaffold that retained the biomechanical properties of the native artery. Initial re-cellularization studies with ovine vascular cells was successful.

scholarly journals Mesenchymal stem cell transplantation for vaginal repair in an ovariectomized rhesus macaque model

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ye Zhang ◽  
Yidi Ma ◽  
Juan Chen ◽  
Min Wang ◽  
Yuan Cao ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Rhesus Macaques ◽  
Biomechanical Properties ◽  
Saline Group ◽  
Vaginal Tissue ◽  
Macaque Model ◽  
Biochemical Analyses

Abstract Background Current surgical therapies for pelvic organ prolapse (POP) do not repair weak vaginal tissue and just provide support; these therapies may trigger severe complications. Stem cell-based regenerative therapy, due to its ability to reconstruct damaged tissue, may be a promising therapeutic strategy for POP. The objective of this study is to evaluate whether mesenchymal stem cell (MSC) therapy can repair weak vaginal tissue in an ovariectomized rhesus macaque model. Methods A bilateral ovariectomy model was established in rhesus macaques to induce menopause-related vaginal injury. Ten bilaterally ovariectomized rhesus macaques were divided into two groups (n=5/group): the saline group and the MSC group. Three months after ovariectomy, saline or MSCs were injected in situ into the injured vaginal wall. The vaginal tissue was harvested 12 weeks after injection for histological and biochemical analyses to evaluate changes of extracellular matrix, microvascular density, and smooth muscle in the vaginal tissue. Biomechanical properties of the vaginal tissue were assessed by uniaxial tensile testing. Data analysis was performed with unpaired Student’s t test or Mann-Whitney. Results Twelve weeks after MSC transplantation, histological and biochemical analyses revealed that the content of collagen I, elastin, and microvascular density in the lamina propria of the vagina increased significantly in the MSC group compared with the saline group. And the fraction of smooth muscle in the muscularis of vagina increased significantly in the MSC group. In addition, MSC transplantation improved the biomechanical properties of the vagina by enhancing the elastic modulus. Conclusion Vaginal MSC transplantation could repair the weak vaginal tissue by promoting extracellular matrix ingrowth, neovascularization, and smooth muscle formation and improve the biomechanical properties of the vagina, providing a new prospective treatment for POP.

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