Changes in calcium concentrations in subcellular compartments of rat submandibular gland acinar cells induced by cholinergic stimulation

1999 ◽  
Vol 112 (5) ◽  
pp. 367-373 ◽  
Author(s):  
A.-L. Zhang ◽  
J. R. Martinez ◽  
G. M. Roomans
Keyword(s):  
Submandibular Gland ◽  
Acinar Cells ◽  
Cholinergic Stimulation ◽  
Subcellular Compartments ◽  
Rat Submandibular Gland

Activation of ouabain-sensitive p-nitrophenylphosphatase by carbachol and cGMP in rat submandibular gland

1980 ◽  
Vol 58 (10) ◽  
pp. 1223-1229 ◽  
Author(s):  
Maggie M. Shi ◽  
D. J. Stewart ◽  
A. K. Sen
Keyword(s):  
Enzyme Activity ◽  
Atpase Activity ◽  
Submandibular Gland ◽  
Second Messenger ◽  
Sodium Ion ◽  
Cholinergic Stimulation ◽  
Minimal Requirement ◽  
Primary Fluid ◽  
Fluid Formation ◽  
Rat Submandibular Gland

Na+,K+-ATPase activity was monitored by measuring ouabain-sensitive K+-dependent p-nitrophenylphosphatase (p-NPPase) activity in rat submandibular gland slices. Carbachol (carbamylcholine chloride) stimulated the p-NPPase activity in the presence of calcium but not in its absence. Carbachol activation of the enzyme was totally ouabain sensitive and could be blocked by atropine. A minimal requirement of sodium ion extracellularly was required for this carbachol stimulation. cGMP and its dibutyryl analogue was also effective in stimulating the enzyme activity, whereas, cAMP was ineffective. Calcium, however, was not required for cGMP activation of the p-NPPase activity. The result indicates that calcium is the second messenger and cGMP is the tertiary connection between cholinergic stimulation and Na+,K+-ATPase activation in these glands. Activation of Na+,K+-ATPase is postulated to be responsible for primary fluid formation.

Cholinergic stimulation of ouabain-sensitive respiration in rat submandibular gland

1983 ◽  
Vol 245 (3) ◽  
pp. G364-G368 ◽  
Author(s):  
D. J. Stewart ◽  
D. J. Pon ◽  
A. K. Sen
Keyword(s):  
Oxygen Consumption ◽  
Submandibular Gland ◽  
Sodium Pump ◽  
Calcium Ionophore ◽  
Sodium Reabsorption ◽  
Tissue Respiration ◽  
Ionophore A23187 ◽  
Cholinergic Stimulation ◽  
Rat Submandibular Gland ◽  
Stimulation Of

Oxygen consumption of slices of rat submandibular gland was monitored with an oxygen electrode method. Carbachol stimulated an immediate increase in tissue respiration that was inhibitable by ouabain. The stimulation required the presence of calcium in the incubation medium and was blocked by atropine. The calcium ionophore A23187 also stimulated ouabain-sensitive oxygen consumption in the tissue slices. The results show that the mechanism using the extra energy during cholinergic stimulation is the sodium pump. Amiloride at a 1, 10, or 100 microM concentration had no effect on stimulation of ouabain-sensitive respiration by carbachol. Since amiloride, which is known to block the sodium reabsorption process in the ductal segment, has no effect on the stimulation, the increased sodium pump activity is probably located in the acinar region and is associated with the primary fluid secretion process.

Effect of PACAP-27 on Adenylate Cyclase in Ductal and Acinar Cells of Rat Submandibular Gland

1998 ◽  
Vol 865 (1 VIP, PACAP, A) ◽  
pp. 431-437 ◽  
Author(s):  
E. KABRE ◽  
N. CHAIB ◽  
H. AMSALLEM ◽  
A. MORAN ◽  
M. C VANDERMEERS ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Submandibular Gland ◽  
Acinar Cells ◽  
Rat Submandibular Gland
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