Activation of neurokinin 1 receptors on interstitial cells of Cajal of the guinea-pig small intestine by substance P

1998 ◽  
Vol 110 (3) ◽  
pp. 263-271 ◽  
Author(s):  
S. T. Lavin ◽  
B. R. Southwell ◽  
R. Murphy ◽  
K. M. Jenkinson ◽  
J. B. Furness
Keyword(s):  
Small Intestine ◽  
Substance P ◽  
Guinea Pig ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Neurokinin 1 ◽  
Cells Of Cajal

Selective labeling and isolation of functional classes of interstitial cells of Cajal of human and murine small intestine

2007 ◽  
Vol 292 (1) ◽  
pp. C497-C507 ◽  
Author(s):  
Hui Chen ◽  
Doug Redelman ◽  
Seungil Ro ◽  
Sean M. Ward ◽  
Tamás Ördög ◽  
...  
Keyword(s):  
Small Intestine ◽  
Substance P ◽  
Interstitial Cells Of Cajal ◽  
Soluble Guanylate Cyclase ◽  
Fluorescent Microscopy ◽  
Circular Muscle ◽  
Interstitial Cells ◽  
Functional Classes ◽  
Neurokinin 1 ◽  
Cells Of Cajal

Specific functions of interstitial cells of Cajal (ICC) have been linked to distinct classes that differ by morphology and distribution. In the small intestine, slow wave-generating ICC are located in the myenteric region (ICC-MY), whereas ICC that mediate neuromuscular neurotransmission occur either throughout the circular muscle layer (intramuscular ICC, ICC-IM) or in association with the deep muscular plexus (ICC-DMP). Selective isolation of ICC to characterize specific properties has been difficult. Recently, neurokinin-1 receptors have been detected in murine ICC-DMP and neurons but not in ICC-MY. Here we identified and isolated ICC-DMP/IM by receptor-mediated internalization of fluorescent substance P and Kit immunofluorescence. Specificity of labeling was verified by confocal microscopy. Mouse and human ICC-DMP/IM were detected in suspension by fluorescent microscopy and harvested for RT-PCR with micropipettes. The isolated cells expressed Kit but not markers for neurons, smooth muscle, or antigen-presenting cells. ICC-DMP expressed neurokinin-1 receptor, M2 and M3 muscarinic receptors, P2Y1 and P2Y4 purinergic receptors, VIP receptor 2, soluble guanylate cyclase-1 subunits, and protein kinase G. L- or T-type Ca2+ channels were not detected in these cells. ICC-MY and ICC-DMP were simultaneously detected and enumerated by flow cytometry and sorted to purity by fluorescence-activated cell sorting. In summary, functional classes of ICC have distinct molecular identities that can be used to selectively identify and harvest these cells with, for example, receptor-mediated uptake of substance P and Kit immunofluorescence. ICC-DMP express neurotransmitter receptors and signaling intermediate molecules that are consistent with their role in neuromuscular neurotransmission.

scholarly journals Histological identification of the interstitial cells of Cajal in the guinea-pig small intestine.

1989 ◽  
Vol 52 (3) ◽  
pp. 267-286 ◽  
Author(s):  
Shigeru KOBAYASHI ◽  
John B. FURNESS ◽  
Terence K. SMITH ◽  
Sueli POMPOLO
Keyword(s):  
Small Intestine ◽  
Guinea Pig ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Cells Of Cajal
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