Electron microscopic immunolocalization of basic fibroblast growth factor in peripheral nerves

2000 ◽  
Vol 114 (5) ◽  
pp. 413-419 ◽  
Author(s):  
Robert J. Kayton ◽  
Ranan Gulhan Aktas
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Peripheral Nerves ◽  
Fibroblast Growth ◽  
Electron Microscopic

Electron Microscopic Immunolocalization of Basic Fibroblast Growth Factor-Like Molecules in Capillary Endothelial Cells

1998 ◽  
Vol 4 (S2) ◽  
pp. 1100-1101
Author(s):  
Ranan Gullhan Aktas ◽  
Robert J. Kayton
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Immunogold Labeling ◽  
Fibroblast Growth ◽  
Electron Microscopic ◽  
Adult Rats

Basic fibroblast growth factor (bFGF) is a potent angiogenic polypeptide. It promotes angiogenesis in vivo and in vitro by stimulating migration, proliferation and proteolytic activity of endothelial cells. Whereas several effects of exogenous bFGF on endothelial cells have been described, it has remained unclear how endogenous bFGF produced by vascular endothelial cells regulate angiogenesis.To further investigate functional implications of the distribution of bFGF, we undertook the present study. Our aims were (i) to identify the specific location of bFGF in endothelial cells using electron microscopy immunogold labeling technique (ii) to determine the distribution of bFGF in capillaries of different types of tissues.Tissue samples from sciatic nerve, hippocampus, adrenal gland and kidney of normal adult rats were fixed in 4% paraformaldehyde/1 to 5% glutaraldehyde and embedded in Spurr's resin. Ultrathin sections were labeled with either polyclonal (F3393-Sigma) or monoclonal antibodies (F6162-Sigma, C3316-ZymoGenetics) specific for bFGF using a two-step immunogold labeling method.

Ultrastructural Distribution of Basic Fibroblast Growth Factor-Like Molecules in Peripheral Nerves

1998 ◽  
Vol 4 (S2) ◽  
pp. 1102-1103
Author(s):  
Robert J. Kayton ◽  
Ranan Gulhan Aktas
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Peripheral Nerves ◽  
Fibroblast Growth ◽  
Adult Rats ◽  
Ultrathin Sections ◽  
Few Data ◽  
Labeling Method

Basic Fibroblast Growth Factor (bFGF) is a multifunctional polypeptide which has been shown to play a pivotal role in the survival and differentiation of nerve cells. Several trophic and non-trophic functions of this protein have been suggested in peripheral nerves. In spite of ample information about the distribution and effects of bFGF in central nervous system, few data are available concerning the localization of this protein in peripheral nerves. In view of the role of bFGF in regulation of trophic and non-trophic functions, we particularly focused on the presence and precise location of bFGF in peripheral nerves at the electron microscope level.Spurr's resin embedded ultrathin sections from adult rats’ sural nerves were labeled with either polyclonal (F3393-Sigma) or monoclonal antibodies (F6162-Sigma, C3316-Zymogenetics) specific for bFGF using two-step immunogold labeling method. Control samples were treated with either an equivalent volume of blocking solution (omitting the primary antibody) or an irrelevant antibody (Factor VIII, VGF, anti-histamine, anti-fibroblast 5B5).

Basic fibroblast growth factor (bFGF) acts intracellularly to cause the transdifferentiation of avian neural crest-derived Schwann cell precursors into melanocytes

Development ◽  
1993 ◽  
Vol 118 (4) ◽  
pp. 1313-1326 ◽  
Author(s):  
L. Sherman ◽  
K.M. Stocker ◽  
R. Morrison ◽  
G. Ciment
Keyword(s):  
Growth Factor ◽  
Schwann Cell ◽  
Neural Crest ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Peripheral Nerves ◽  
Neutralizing Antibodies ◽  
Fibroblast Growth ◽  
Mrna Transcript ◽  
Schwann Cell Precursors

We previously found that cultured neural crest-derived cells from embryonic quail peripheral nerves, which consist mostly of Schwann cell precursors, gave rise to melanocytes following treatment with basic fibroblast growth factor (bFGF) or 12-O-tetradecanoyl phorbol-13-acetate (TPA). Here, we show that antisense deoxyoligonucleotides targeted against two regions of the bFGF mRNA transcript blocked this TPA-induced transdifferentiation of Schwann cell precursors. Neither sense nor scrambled antisense control oligonucleotides had any effect in this regard. TPA increased bFGF protein expression in cell lysates but not in conditioned media from these cultures, and this expression was localized to the nucleus and cytoplasm. Furthermore, bFGF-neutralizing antibodies and inositol-hexakisphosphate (InsP6) both inhibited pigmentation caused by exogenous bFGF, but had no affect on TPA-induced melanogenesis, suggesting that bFGF is not released by these cells. These data indicate that bFGF is necessary for the TPA-induced transdifferentiation of Schwann cell precursors into melanocytes and that bFGF acts via an intracrine mechanism.

A Case of Contact Dermatitis due to Fiblast Spray, a Product of Basic Fibroblast Growth Factor

2006 ◽  
Vol 68 (3) ◽  
pp. 248-250 ◽  
Author(s):  
Shuko OKADA ◽  
Takashi MASU ◽  
Takahiko TSUNODA ◽  
Ryuhei OKUYAMA ◽  
Setsuya AIBA
Keyword(s):  
Contact Dermatitis ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth
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