Use of a serum-free medium for long-term storage of human corneas. Influence on endothelial cell density and corneal metabolism

2001 ◽  
Vol 239 (10) ◽  
pp. 801-805 ◽  
Author(s):  
Britta Hempel ◽  
Jürgen Bednarz ◽  
Katrin Engelmann
Keyword(s):  
Endothelial Cell ◽  
Cell Density ◽  
Endothelial Cell Density ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Long Term Storage ◽  
Term Storage ◽  
Corneal Metabolism

Cell density affects spreading and clustering, but not attachment, of human keratinocytes in serum-free medium

1991 ◽  
Vol 99 (2) ◽  
pp. 387-395
Author(s):  
M. Malcovati ◽  
M.L. Tenchini
Keyword(s):  
Cell Density ◽  
Time Course ◽  
Cluster Formation ◽  
Physicochemical Characterization ◽  
Human Keratinocytes ◽  
Conditioned Media ◽  
Serum Free Medium ◽  
Free Medium ◽  
Spreading Factor ◽  
Serum Free

Attachment, spreading and clustering of second-passage human human keratinocytes in serum-free medium have been evaluated within 24 h after plating, as a function of the density of the inoculum and of time, in two different strains. The results show that attachment is unaffected by cell density and differs significantly from strain to strain. Cell density affects the distribution of attached keratinocytes among three morphologically distinct classes: unspread, spread and clustered cells. The percentage of unspread keratinocytes shows a linear decrease at increasing cell density, and that of spread keratinocytes an increase, resulting from statistically significant increases in the percentages of both single and clustered cells. Spreading on uncoated surfaces appears therefore as an inducible phenomenon. The use of media conditioned by keratinocytes, fibroblasts and HeLa cells shows that keratinocytes specifically secrete a diffusible ‘spreading factor’. We term this phenomenon ‘autocrine induced spreading’. Preliminary physicochemical characterization suggests that a protein could be responsible for the spreading activity of conditioned media. The ‘spreading factor’ seems to act directly on the cells, and not through a modification of the plastic surface of the dishes, since most (greater than 70%) of the spreading activity can be recovered in the conditioned media used in pre-coating experiments. The percentages of clusters follow ‘saturation’ kinetics at increasing cell density, while the percentage of clustered cells increases linearly with the density of inoculum. Time-course experiments show that the rate of spreading is cell density- and strain-independent. The percentages of clusters and of total clustered cells are time-independent, suggesting that cluster formation takes place in suspension.(ABSTRACT TRUNCATED AT 250 WORDS)

Cases With Long-Term High Corneal Endothelial Cell Density Maintained After Corneal Transplantation

Cornea ◽  
2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Kanae Kayukawa ◽  
Koji Kitazawa ◽  
Koichi Wakimasu ◽  
Sanjay V. Patel ◽  
John Bush ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Density ◽  
Corneal Transplantation ◽  
Corneal Endothelial Cell ◽  
Endothelial Cell Density ◽  
Corneal Endothelial Cell Density

Primary culture of human diploid cells and its long-term transfer in a serum-free medium

1981 ◽  
Vol 134 (2) ◽  
pp. 470-474 ◽  
Author(s):  
Isao Yamane ◽  
Mikio Kan ◽  
Hiroyoshi Hoshi ◽  
Yoshiki Minamoto
Keyword(s):  
Primary Culture ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Human Diploid Cells ◽  
Diploid Cells
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