Monte Carlo simulation of DNA strand-break induction in supercoiled plasmid pBR322 DNA from indirect effects

1998 ◽  
Vol 36 (4) ◽  
pp. 235-241 ◽  
Author(s):  
H. Tomita ◽  
M. Kai ◽  
T. Kusama ◽  
A. Ito
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Indirect Effects ◽  
Strand Break ◽  
Plasmid Pbr322 ◽  
Supercoiled Plasmid ◽  
Dna Strand Break ◽  
Pbr322 Dna ◽  
Dna Strand

The invariance of the total direct DNA strand break yield

2011 ◽  
Vol 38 (7) ◽  
pp. 4147-4153 ◽  
Author(s):  
M. A. Bernal ◽  
C. E. deAlmeida ◽  
C. Sampaio ◽  
S. Incerti ◽  
C. Champion ◽  
...  
Keyword(s):  
Strand Break ◽  
Dna Strand Break ◽  
Dna Strand ◽  
Break Yield

scholarly journals Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties

2020 ◽  
Author(s):  
Katja Maria Bendtsen ◽  
Louise Gren ◽  
Vilhelm Berg Malmborg ◽  
Pravesh Chandra Shukla ◽  
Martin Tunér ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Diesel Exhaust ◽  
Strand Break ◽  
Diesel Exhaust Particles ◽  
Ultra Low Sulfur Diesel ◽  
Dna Strand Break ◽  
Diesel Particles ◽  
Exhaust Particles ◽  
Dna Strand ◽  
Low Sulfur

Abstract Background Diesel exhaust is carcinogenic and exposure to diesel particles cause health effects. We investigated the toxicity of diesel exhaust particles designed to have varying physicochemical properties in order to attribute health effects to specific particle characteristics. Particles from three fuel types were compared at 13% engine intake O 2 concentration: MK1 ultra low sulfur diesel (DEP13) and the two renewable diesel fuels hydrotreated vegetable oil (HVO13) and rapeseed methyl ester (RME13). Additionally, diesel particles from MK1 ultra low sulfur diesel were generated at 9.7% (DEP9.7) and 17% (DEP17) intake O 2 concentration. We evaluated physicochemical properties and histopathological, inflammatory and genotoxic responses on day 1, 28 and 90 after single intratracheal instillation in mice compared to reference diesel particles and carbon black.Results Moderate variations were seen in physical properties for the five particles: primary particle diameter: 15-22 nm, specific surface area: 152-222 m 2 /g, and count median mobility diameter: 55-103 nm. Larger differences were found in chemical composition: organic carbon/total carbon ratio (0.12-0.60), polycyclic aromatic hydrocarbon content (1-27 mg/mg) and acid-extractable metal content (0.9-16 mg/mg). Intratracheal exposure to all five particles induced similar toxicological responses, with different potency. Lung particle retention was observed in DEP13 and HVO13 exposed mice on day 28, with less retention for the other fuel types. RME exposure induced limited response whereas the remaining particles induced dose-dependent inflammation and acute phase response on day 1. DEP13 induced acute phase response on day 28 and inflammation on day 90. DNA strand break levels were not increased as compared to vehicle, but were increased in lung and liver compared to blank filter extraction control. Neutrophil influx on day 1 correlated best with estimated deposited surface area, but also with elemental carbon, organic carbon and PAHs. DNA strand break levels in liver on day 90 correlated with acellular particle-induced ROS.Conclusions We studied diesel exhaust particles designed to differ in physicochemical properties. Our study highlights particle size, elemental carbon content, PAHs and ROS-generating potential as physicochemical predictors of diesel particle toxicity.

scholarly journals Impact of PNKP mutations associated with microcephaly, seizures and developmental delay on enzyme activity and DNA strand break repair

2012 ◽  
Vol 40 (14) ◽  
pp. 6608-6619 ◽  
Author(s):  
John J. Reynolds ◽  
Alexandra K. Walker ◽  
Edward C. Gilmore ◽  
Christopher A. Walsh ◽  
Keith W. Caldecott
Keyword(s):  
Enzyme Activity ◽  
Developmental Delay ◽  
Strand Break ◽  
Dna Strand Break ◽  
Break Repair ◽  
Dna Strand
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