A new HPLC-based method for the quantitative analysis of inner stratum corneum lipids with special reference to the free fatty acid fraction

1998 ◽  
Vol 290 (9) ◽  
pp. 508-516 ◽  
Author(s):  
L. Norlén ◽  
Ingrid Nicander ◽  
Anders Lundsjö ◽  
Tomas Cronholm ◽  
Bo Forslind
Keyword(s):  
Fatty Acid ◽  
Quantitative Analysis ◽  
Free Fatty Acid ◽  
Stratum Corneum ◽  
Special Reference ◽  
Fatty Acid Fraction ◽  
Free Fatty Acid Fraction ◽  
Acid Fraction ◽  
Stratum Corneum Lipids

Changes in the lipid content and fatty acid composition of 2nd-stage juveniles of Globodera rostochiensis after rehydration, exposure to the hatching stimulus and hatch

Parasitology ◽  
1998 ◽  
Vol 116 (2) ◽  
pp. 183-190 ◽  
Author(s):  
R. A. HOLZ ◽  
D. J. WRIGHT ◽  
R. N. PERRY
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Acid Composition ◽  
Lipid Content ◽  
Monounsaturated Fatty Acids ◽  
Fatty Acid Fraction ◽  
Acid Fraction

The total lipid content of the dry weight of whole cysts and 2nd-stage juveniles (J2) of Globodera rostochiensis was 17·1% in dry cysts, 20·9% in cysts soaked in distilled water (DW), 20·3% in cysts that had been in potato root diffusate (PRD) for 7 days, 7·3% in cysts that had been in PRD for 28 days and 29·2% for hatched J2. The fatty acid composition of the total lipid did not differ between dry cysts, cysts in DW and cysts in PRD for 7 days. However, major differences in the fatty acid composition of all lipid classes were found between rehydrated cysts in PRD and freshly hatched J2. After hatching, the degree of saturation and the percentage of monounsaturated fatty acids decreased and the percentage of polyunsaturated fatty acids increased considerably, especially in the free fatty acid fraction, where C20[ratio ]1 showed an 8-fold decrease and C20[ratio ]4 a 33-fold increase. There was a difference in the fatty acid composition of cysts in PRD for 7 days and cysts in PRD for 28 days (after most of the J2 had hatched); with increased time in PRD the percentage of polyunsaturated fatty acids increased and the percentage of monounsaturated fatty acids decreased in all lipid classes. Differences in the fatty acid profiles between cysts in PRD for 28 days and hatched J2 were found mainly in the free fatty acid and the non-acidic phospholipid fractions. The free fatty acid fraction of the cysts was dominated by monounsaturated fatty acids (52%), whereas the same fraction of hatched J2 contained large amounts of polyunsaturated fatty acids (60%). These polyunsaturated fatty acids, especially C20[ratio ]4, might play an important part in nematode–plant interactions during infection.

The crop as an organ of glyceride absorption in the American cockroach, Periplaneta americana L.

1976 ◽  
Vol 54 (7) ◽  
pp. 1165-1171 ◽  
Author(s):  
A. G. D. Hoffman ◽  
R. G. H. Downer
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Palmitic Acid ◽  
Test Meal ◽  
Periplaneta Americana ◽  
Fatty Acid Fraction ◽  
American Cockroach ◽  
Acid Fraction ◽  
Triglyceride Hydrolysis

The potential of the crop to absorb the products of triglyceride hydrolysis was investigated in the American cockroach, Periplaneta americana. Isolated sacs of crop and midgut tissue were filled with test loads containing [1-14C]palmitic acid or glyceryl tri[1-14C]palmitic acid and the efflux of label monitored. Label from both preparations was recovered primarily from the free fatty acid fraction. Inclusion of midgut lipase enhanced the rate of 14C efflux from test loads containing labelled triglycerides. Comparison of the relative rates of 14C efflux from crop and midgut sacs shows that the greatest efflux occurs from midgut, although the crop possesses the potential to effect absorption of significant amounts of hydrolysed triglyceride. The capacity of crop to absorb fatty acids was confirmed by monitoring the evolution of 14CO2 from insects in which the crop was ligated anterior to the proventriculus before they were fed a test meal containing [1-14C]palmitic acid. Ligated insects evolved 41.5% of the 14CO2 produced by non-ligated insects.

scholarly journals AN UNSATURATED FATTY ACID FRACTION OF PIG PANCREAS WHICH INHIBITS THE GROWTH OF CHICKEN SARCOMA

1936 ◽  
Vol 64 (3) ◽  
pp. 333-338 ◽  
Author(s):  
O. M. Helmer
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Iodine Number ◽  
Aqueous Extract ◽  
Unsaturated Fatty Acid ◽  
Fatty Acid Fraction ◽  
Pancreatic Tissue ◽  
Acid Number ◽  
Acid Fraction ◽  
Commercial Oleic Acid

The inhibiting action of pancreatic tissue was found to be associated with the unsaturated fatty acid fraction. As small an amount of fatty acid as 0.1 mg. inhibited the chicken sarcoma agent contained in 0.2 cc. of a 1:60 aqueous extract of Chicken Tumor I. The unsaturated fatty acid had an acid number and an iodine number similar to those for oleic acid. Commercial oleic acid also was found to inhibit the growth of the chicken sarcoma in comparable quantities.

Effects of addition of clay minerals on the fatty acid fraction of a podzol soil

1995 ◽  
Vol 46 (2) ◽  
pp. 187-192 ◽  
Author(s):  
P. JAMBU ◽  
A. AMBLES ◽  
P. MAGNOUX ◽  
E. PARLANTI
Keyword(s):  
Fatty Acid ◽  
Clay Minerals ◽  
Fatty Acid Fraction ◽  
Acid Fraction
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