Expression of nitric oxide synthase III (eNOS) mRNA by human skin cells: melanocytes but not keratinocytes express eNOS mRNA

1998 ◽  
Vol 290 (6) ◽  
pp. 350-352 ◽  
Author(s):  
M. Jackson ◽  
Fiona Frame ◽  
Richard Weller ◽  
Roderick C. McKenzie
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Human Skin ◽  
Skin Cells ◽  
Enos Mrna ◽  
Human Skin Cells ◽  
Oxide Synthase

scholarly journals Inhibitors of Nitric Oxide Synthase in Human Skin

1996 ◽  
Vol 106 (1) ◽  
pp. 113-118 ◽  
Author(s):  
Portia C. Goldsmith ◽  
Tabi A. Leslie ◽  
Nicholas A. Hayes ◽  
Nicholas J. Levell ◽  
Pauline M. Dowd ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Human Skin ◽  
Oxide Synthase

Retinoic acid suppression of endothelial nitric oxide synthase in porcine oocyte

2002 ◽  
Vol 80 (8) ◽  
pp. 777-782 ◽  
Author(s):  
Masa-aki Hattori ◽  
Yukio Kato ◽  
Noboru Fujihara
Keyword(s):  
Nitric Oxide ◽  
Retinoic Acid ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Mrna Level ◽  
Dependent Manner ◽  
Hormonal Stimulation ◽  
Enos Mrna ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

The presence of endothelial nitric oxide synthase (eNOS) has been found in porcine oocytes, but its mRNA and protein levels remain relatively constant during hormonal stimulation. The present study was designed to determine the effect of retinoic acid on eNOS regulation in porcine oocytes during follicle-stimulating hormone (FSH) stimulation. Cumulus–oocyte complexes (COCs), prepared from small antral follicles of immature porcine ovaries, were cultured for 15 h and treated with FSH for an additional 48 h. eNOS mRNA and its protein were analyzed by reverse transcription – polymerase chain reaction and Western blotting, respectively. Retinoic acid had an inhibitory effect on the level of oocyte eNOS mRNA in a dose-dependent manner if COCs were exposed to retinoic acid before FSH stimulation. The inhibition of FSH action was reflected in a decrease in expression of c-fos mRNA. eNOS protein also decreased to approximately 50% of the control after exposure to 10 μM retinoic acid. However, the ability of NO synthesis was abolished in the oocytes prepared from retinoic acid pretreated COCs. These results suggest that retinoic acid has a strong inhibitory action on eNOS mRNA level and NO synthesis in the porcine oocyte.Key words: oocyte, retinoic acid, NO synthesis, eNOS, RT–PCR.

Expression of endothelial nitric oxide synthase in the postnatal developing porcine kidney

2001 ◽  
Vol 280 (5) ◽  
pp. R1269-R1275 ◽  
Author(s):  
Michael J. Solhaug ◽  
Usa Kullaprawithaya ◽  
Xui Q. Dong ◽  
Ke-Wen Dong
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Protein Content ◽  
Endothelial Nitric Oxide Synthase ◽  
Enos Expression ◽  
Developmentally Regulated ◽  
Enos Mrna ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide ◽  
Enos Protein

The postnatal pattern of renal endothelial nitric oxide synthase (eNOS) is unknown. The purpose of this study was to characterize eNOS expression during maturation and compare this to neuronal NOS (nNOS). The experiments measured whole kidney eNOS mRNA expression by RT-PCR and protein content by Western blot, as well as cortical and medullary protein content in piglets at selected postnatal ages and in adult pigs. Whole kidney eNOS mRNA was compared with nNOS. Whole kidney eNOS expression decreased from the newborn to its lowest at 7 days, returning by 14 days to adult levels. This eNOS mRNA pattern contrasted with nNOS, which was highest at birth, and progressively decreased to its lowest level in the adult. At birth, cortical eNOS protein was greater than medullary, contrasting with the adult pattern of equivalent levels. In conclusion eNOS is developmentally regulated during early renal maturation and may critically participate in renal function during this period. The eNOS developmental pattern differs from nNOS, suggesting that these isoforms may have different regulatory factors and functional contributions in the postnatal kidney.

Expression of nitric oxide synthase isoforms is reduced in late-gestation ovine fetal brainstem

2005 ◽  
Vol 289 (2) ◽  
pp. R613-R619 ◽  
Author(s):  
Charles E. Wood ◽  
Gin-Fu Chen ◽  
Maureen Keller-Wood
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Vascular Endothelial Cells ◽  
Late Gestation ◽  
Inos Mrna ◽  
Fetal Sheep ◽  
Enos Mrna ◽  
Ovine Fetal ◽  
Oxide Synthase ◽  
Nos Isoforms

Fetal baroreflex responsiveness increases in late gestation. An important modulator of baroreflex activity is the generation of nitric oxide in the brainstem nuclei that integrate afferent and efferent reflex activity. The present study was designed to test the hypothesis that nitric oxide synthase (NOS) isoforms are expressed in the fetal brainstem and that the expression of one or more of these enzymes is reduced in late gestation. Brainstem tissue was rapidly collected from fetal sheep of known gestational ages (80, 100, 120, 130, 145 days gestation and 1 day and 1 wk postnatal). Neuronal (nNOS), inducible (iNOS), and endothelial (eNOS) mRNA was measured using real-time PCR methodology specific for ovine NOS isoforms. The three enzymes were measured at the protein level using Western blot methodology. In tissue prepared for histology separately, the cellular pattern of immunostaining was identified in medullae from late-gestation fetal sheep. Fetal brainstem contained mRNA and protein of all three NOS isoforms, with nNOS the most abundant, followed by iNOS and eNOS, respectively. nNOS and iNOS mRNA abundances were highest at 80 days' gestation, with statistically significant decreases in abundance in more mature fetuses and postnatal animals. nNOS and eNOS protein abundance also decreased as a function of developmental age. nNOS and eNOS were expressed in neurons, iNOS was expressed in glia, and eNOS was expressed in vascular endothelial cells. We conclude that all three isoforms of NOS are constitutively expressed within the fetal brainstem, and the expression of all three forms is reduced with advancing gestation. We speculate that the reduced expression of NOS in this brain region plays a role in the increased fetal baroreflex activity in late gestation.

Biological effects of nitric oxide generated by an atmospheric pressure gas-plasma on human skin cells

Nitric Oxide ◽  
2011 ◽  
Vol 24 (1) ◽  
pp. 8-16 ◽  
Author(s):  
Joerg Liebmann ◽  
Joachim Scherer ◽  
Nikita Bibinov ◽  
Priyadarshini Rajasekaran ◽  
Reinhold Kovacs ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Human Skin ◽  
Atmospheric Pressure ◽  
Biological Effects ◽  
Skin Cells ◽  
Gas Plasma ◽  
Human Skin Cells

Nitric oxide synthase; Immunolocalisation and function in normal human skin

1993 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
PC Goldsmith ◽  
NJ Levell ◽  
JC Foreman ◽  
Pauline M. Dowd
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Human Skin ◽  
Normal Human Skin ◽  
Normal Human ◽  
And Function ◽  
Oxide Synthase
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