The promoter of the HaCaT keratinocyte differentiation-related gene keratin 4 contains a functional AP-2 binding site

1997 ◽  
Vol 289 (12) ◽  
pp. 705-708 ◽  
Author(s):  
R. Wanner ◽  
Juan Zhang ◽  
Tomislav Dorbic ◽  
Dietmar Mischke ◽  
Beate M. Henz ◽  
...  
Keyword(s):  
Binding Site ◽  
Keratinocyte Differentiation ◽  
Related Gene ◽  
Hacat Keratinocyte

scholarly journals Konjac Ceramide (kCer)-Mediated Signal Transduction of the Sema3A Pathway Promotes HaCaT Keratinocyte Differentiation

Biology ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 121
Author(s):  
Seigo Usuki ◽  
Noriko Tamura ◽  
Tomohiro Tamura ◽  
Kohei Yuyama ◽  
Daisuke Mikami ◽  
...  
Keyword(s):  
Cell Migration ◽  
Cell Differentiation ◽  
Intracellular Signaling ◽  
Keratinocyte Differentiation ◽  
Hacat Cells ◽  
Differentiation Markers ◽  
Signaling Mechanism ◽  
Protein Levels ◽  
Intracellular Signaling Cascade ◽  
Hacat Keratinocyte

Histamines suppress epidermal keratinocyte differentiation. Previously, we reported that konjac ceramide (kCer) suppresses histamine-stimulated cell migration of HaCaT keratinocytes. kCer specifically binds to Nrp1 and does not interact with histamine receptors. The signaling mechanism of kCer in HaCaT cells is also controlled by an intracellular signaling cascade activated by the Sema3A-Nrp1 pathway. In the present study, we demonstrated that kCer treatment induced HaCaT keratinocyte differentiation after migration of immature cells. kCer-induced HaCaT cell differentiation was accompanied by some features of keratinocyte differentiation markers. kCer induced activating phosphorylation of p38MAPK and c-Fos, which increased the protein levels of involucrin that was the latter differentiation marker. In addition, we demonstrated that the effects of both kCer and histamines are regulated by an intracellular mechanism of Rac1 activation/RhoA inhibition downstream of the Sema3A/Nrp1 receptor and histamine/GPCR pathways. In summary, the effects of kCer on cell migration and cell differentiation are regulated by cascade crosstalk between downstream Nrp1 and histamine-GPCR pathways in HaCaT cells.

Identification and cornification-related gene expression of canine keratinocyte differentiation-associated protein, Kdap

2006 ◽  
Vol 172 (1) ◽  
pp. 141-146 ◽  
Author(s):  
H. Yagihara ◽  
Y. Terada ◽  
S. Sugimoto ◽  
F. Hidaka ◽  
O. Yamada ◽  
...  
Keyword(s):  
Gene Expression ◽  
Keratinocyte Differentiation ◽  
Related Gene

scholarly journals Mapping the Binding Site of a Humanether-a-go-go-related Gene-specific Peptide Toxin (ErgTx) to the Channel's Outer Vestibule

2002 ◽  
Vol 277 (19) ◽  
pp. 16403-16411 ◽  
Author(s):  
Liliana Pardo-Lopez ◽  
Mei Zhang ◽  
Jie Liu ◽  
Min Jiang ◽  
Lourival D. Possani ◽  
...  
Keyword(s):  
Binding Site ◽  
Related Gene ◽  
Outer Vestibule ◽  
Peptide Toxin ◽  
Specific Peptide

Mapping the putative binding site for uPA protein in Esophageal Cancer-Related Gene 2 by heteronuclear NMR method

2008 ◽  
Vol 479 (2) ◽  
pp. 153-157 ◽  
Author(s):  
Yong Geng ◽  
Yingang Feng ◽  
Tao Xie ◽  
Yuanyuan Dai ◽  
Jinfeng Wang ◽  
...  
Keyword(s):  
Esophageal Cancer ◽  
Binding Site ◽  
Heteronuclear Nmr ◽  
Related Gene ◽  
Nmr Method ◽  
Cancer Related Gene ◽  
Putative Binding Site
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