Effect of lactic acid isomers on keratinocyte ceramide synthesis, stratum corneum lipid levels and stratum corneum barrier function

1996 ◽  
Vol 288 (7) ◽  
pp. 383-390 ◽  
Author(s):  
A. V. Rawlings ◽  
A. Davies ◽  
M. Carlomusto ◽  
S. Pillai ◽  
K. Zhang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Stratum Corneum ◽  
Barrier Function ◽  
Lipid Levels ◽  
Stratum Corneum Lipid ◽  
Ceramide Synthesis

Effect of lactic acid isomers on keratinocyte ceramide synthesis, stratum corneum lipid levels and stratum corneum barrier function

1996 ◽  
Vol 288 (7) ◽  
pp. 383-390 ◽  
Author(s):  
A. V. Rawlings ◽  
A. Davies ◽  
M. Carlomusto ◽  
S. Pillai ◽  
K. Zhang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Stratum Corneum ◽  
Barrier Function ◽  
Lipid Levels ◽  
Stratum Corneum Lipid ◽  
Ceramide Synthesis

Aging and the seasons influence stratum corneum lipid levels

1993 ◽  
Vol 6 (1) ◽  
pp. 108 ◽  
Author(s):  
A. Rawlings ◽  
A. Mayo ◽  
J. Rogers ◽  
I. Scott
Keyword(s):  
Stratum Corneum ◽  
Lipid Levels ◽  
Stratum Corneum Lipid

scholarly journals The Effect of a Ceramide-Containing Product on Stratum Corneum Lipid Levels in Dry Legs

2020 ◽  
Vol 19 (4) ◽  
pp. 372-376
Author(s):  
Zoe Draelos ◽  
Nada Baalbaki ◽  
Shelby Cook ◽  
Susana Raab ◽  
Gene Colón
Keyword(s):  
Stratum Corneum ◽  
Lipid Levels ◽  
Stratum Corneum Lipid

scholarly journals Reduced Skin Barrier Function Parallels Abnormal Stratum Corneum Lipid Organization in Patients with Lamellar Ichthyosis

1995 ◽  
Vol 105 (4) ◽  
pp. 619-624 ◽  
Author(s):  
Adriana P.M. Lavrijsen ◽  
Joke A. Bouwstra ◽  
Gert S. Gooris ◽  
Arij Weerheim ◽  
Harry E. Boddé ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Barrier Function ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Lamellar Ichthyosis ◽  
Stratum Corneum Lipid ◽  
Lipid Organization

scholarly journals Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS

2020 ◽  
Vol 61 (6) ◽  
pp. 884-895 ◽  
Author(s):  
Momoko Kawana ◽  
Masatoshi Miyamoto ◽  
Yusuke Ohno ◽  
Akio Kihara
Keyword(s):  
Stratum Corneum ◽  
Barrier Function ◽  
Normal Skin ◽  
Skin Barrier ◽  
Hydroxyl Group ◽  
Skin Barrier Function ◽  
Elongation Cycle ◽  
Barrier Formation ◽  
Ceramide Synthesis

Ceramides are the predominant lipids in the stratum corneum (SC) and are crucial components for normal skin barrier function. Although the composition of various ceramide classes in the human SC has been reported, that in mice is still unknown, despite mice being widely used as animal models of skin barrier function. Here, we performed LC/MS/MS analyses using recently available ceramide class standards to measure 25 classes of free ceramides and 5 classes of protein-bound ceramides from human and mouse SC. Phytosphingosine- and 6-hydroxy sphingosine-type ceramides, which both contain an additional hydroxyl group, were abundant in the human SC (35% and 45% of total ceramides, respectively). In contrast, in mice, phytosph­ingosine- and 6-hydroxy sphingosine-type ceramides were present at ∼1% and undetectable levels, respectively, and sphingosine-type ceramides accounted for ∼90%. In humans, ceramides containing α-hydroxy FA were abundant, whereas ceramides containing β-hydroxy or ω-hydroxy FA were abundant in mice. The hydroxylated β-carbon in β-hydroxy ceramides was in the (R) configuration. Genetic knockout of β-hydroxy acyl-CoA dehydratases in HAP1 cells increased β-hydroxy ceramide levels, suggesting that β-hydroxy acyl-CoA, an FA-elongation cycle intermediate in the ER, is a substrate for β-hydroxy ceramide synthesis. We anticipate that our methods and findings will help to elucidate the role of each ceramide class in skin barrier formation and in the pathogenesis of skin disorders.

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