Ultrastructural localization of Fras1 in the sublamina densa of embryonic epithelial basement membranes

2007 ◽  
Vol 299 (7) ◽  
pp. 337-343 ◽  
Author(s):  
Yannis Dalezios ◽  
Babis Papasozomenos ◽  
Petros Petrou ◽  
Georges Chalepakis
Keyword(s):  
Ultrastructural Localization ◽  
Basement Membranes ◽  
Epithelial Basement

scholarly journals Ultrastructural localization of type V collagen in rat kidney.

1982 ◽  
Vol 92 (2) ◽  
pp. 343-349 ◽  
Author(s):  
A Martinez-Hernandez ◽  
S Gay ◽  
E J Miller
Keyword(s):  
Type I Collagen ◽  
Rat Kidney ◽  
Ultrastructural Localization ◽  
Particulate Material ◽  
Basement Membranes ◽  
Type I ◽  
Type V Collagen ◽  
Collagen Molecules ◽  
Immunohistochemical Studies ◽  
Type V

Antibodies specific for the alpha 1 (V) chain and native collagen molecules containing the alpha 1 (V) chain have been used in electron immunohistochemical studies of rat kidney to determine the ultrastructural distribution of this class of collagen molecules. In addition, antibodies against type I collagen and whole basement membrane were used as markers for interstitial collagen and authentic basement membranes. Our results indicate that type V collagen is present in the renal interstitium in different forms: in close apposition to interstitial collagen fibers; in the stromal aspect of vascular basement membranes; and as particulate material not bound to other structures. On the basis of these findings, we postulate a binding or connecting function for this collagen type.

Hemicentin, a conserved extracellular member of the immunoglobulin superfamily, organizes epithelial and other cell attachments into oriented line-shaped junctions

Development ◽  
2001 ◽  
Vol 128 (6) ◽  
pp. 883-894 ◽  
Author(s):  
B.E. Vogel ◽  
E.M. Hedgecock
Keyword(s):  
Matrix Protein ◽  
Chromosome Instability ◽  
Basement Membranes ◽  
Extracellular Matrix Protein ◽  
Immunoglobulin Superfamily ◽  
Cell Contact ◽  
Chromosome Loss ◽  
Oriented Line ◽  
Escape Reflex ◽  
Epithelial Basement

him-4 mutations cause a novel syndrome of tissue fragility, defective cell migration and chromosome instability in Caenorhabditis elegans. Null mutants have abnormal escape reflex, mispositioning of the vas deferens and uterus, and mitotic chromosome loss and multinucleate cells in the germline. The him-4 gene product, hemicentin, is a conserved extracellular matrix protein with 48 tandem immunoglobulin repeats flanked by novel terminal domains. Secreted from skeletal muscle and gonadal leader cells, hemicentin assembles into fine tracks at specific sites, where it contracts broad regions of cell contact into oriented linear junctions. Some tracks organize hemidesmosomes in the overlying epidermis. Hemicentin tracks facilitate mechanosensory neuron anchorage to the epidermis, gliding of the developing gonad along epithelial basement membranes and germline cellularization.

scholarly journals Ultrastructural localization of the major components of the extracellular matrix in normal rat nerve.

1992 ◽  
Vol 40 (6) ◽  
pp. 859-868 ◽  
Author(s):  
P Lorimier ◽  
P Mezin ◽  
F Labat Moleur ◽  
N Pinel ◽  
S Peyrol ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Peripheral Nerve ◽  
Ultrastructural Localization ◽  
Basement Membranes ◽  
Type I ◽  
Immunoperoxidase Method ◽  
Adult Rat ◽  
Normal Rat ◽  
Rat Nerve ◽  
Fibronectin Type

In this study we determined the ultrastructural distribution of the various components of the extracellular matrix (laminin, fibronectin, Type I, III, and IV collagens) of the normal peripheral nerve in adult rat. The localization of these macromolecules was investigated in basement membranes as well as in different areas of epi-, peri-, and endoneurium, by use of a pre-embedding immunoperoxidase method.

scholarly journals Proteoglycans in primate arteries. I. Ultrastructural localization and distribution in the intima.

1975 ◽  
Vol 67 (3) ◽  
pp. 660-674 ◽  
Author(s):  
T N Wight ◽  
R Ross
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Ruthenium Red ◽  
Basement Membranes ◽  
Macaca Nemestrina ◽  
Elastic Fibers ◽  
Surface Coat ◽  
The Matrix

Proteoglycans were identified and localized histochemically and ultrastructurally in normal and hyperplastic arterial intimas in nonhuman primates (Macaca nemestrina). These regions were consistently more alcianophilic than the adjacent medial layers and this alcianophilia was absent after treatment with glycosaminoglycan-degradative enzymes. Ultrastructurally, the intimal intercellular matrix consisted of numerous, irregularly shaped, 200-500-A diameter granules possessing 30--60-A diameter filamentous projections, and these granules were dispersed between collagen and elastic fibers. The granules exhibited a marked affinity for ruthenium red and were interconnected via their filamentous projections. The ruthenium red-positive granules were intimately associated with the plasma membrane of intimal smooth muscle cells and attached to collagen fibrils and elastic fibers. The matrix granules were completely removed after testicular hyaluronidase or chondroitinase ABC digestion but only partially removed after leech hyaluronidase treatment. These results suggest that the matrix granules contain some hyaluronic acid and one or more isomers of chondroitin sulfate. In addition to the large ruthenium red-positive matrix granules, a smaller class of ruthenium red-positive granule (100--200-A diameter) was present within the basement membranes beneath the endothelium and surrounding the smooth muscle cells. Ruthenium red also exhibited an affinity for the surface coat of the smooth muscle cells. The potential importance of proteoglycans in arterial intimal hyperplasia is discussed.

scholarly journals Loss of surface and cyst epithelial basement membranes and preneoplastic morphologic changes in prophylactic oophorectomies

Cancer ◽  
2003 ◽  
Vol 98 (12) ◽  
pp. 2607-2623 ◽  
Author(s):  
Isabelle H. Roland ◽  
Wan-Lin Yang ◽  
Dong-Hua Yang ◽  
Mary B. Daly ◽  
Robert F. Ozols ◽  
...  
Keyword(s):  
Basement Membranes ◽  
Epithelial Basement ◽  
Morphologic Changes

Cleft palate formation after palatal fusion occurs due to the rupture of epithelial basement membranes

2018 ◽  
Vol 46 (12) ◽  
pp. 2027-2031 ◽  
Author(s):  
Chisato Sakuma ◽  
Hideto Imura ◽  
Tomohiro Yamada ◽  
Toshio Sugahara ◽  
Azumi Hirata ◽  
...  
Keyword(s):  
Cleft Palate ◽  
Basement Membranes ◽  
Palatal Fusion ◽  
Epithelial Basement

Ultrastructural localization of lectin-binding sites in different basement membranes

1993 ◽  
Vol 25 (6) ◽  
pp. 464-468 ◽  
Author(s):  
Mehrdad Salamat ◽  
Werner G�tz ◽  
J�rgen Werner ◽  
Rainer Merken
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Ultrastructural Localization ◽  
Basement Membranes ◽  
Lectin Binding Sites
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