Aging abolishes the cardioprotective effect of combination heat shock and hypoxic preconditioning in reperfused rat hearts

2002 ◽  
Vol 97 (6) ◽  
pp. 489-495 ◽  
Author(s):  
Yukako Honma ◽  
Masato Tani ◽  
Michiyo Takayama ◽  
Ken Yamamura ◽  
Hiroshi Hasegawa
Keyword(s):  
Heat Shock ◽  
Hypoxic Preconditioning ◽  
Cardioprotective Effect ◽  
Rat Hearts

Prior treatment with heat shock attenuates the stress response in isolated working rat hearts

1995 ◽  
Vol 73 (1-2) ◽  
pp. 31-39
Author(s):  
Todd A. Dakin ◽  
R. William Currie
Keyword(s):  
Heat Shock ◽  
Control Mechanism ◽  
Negative Control ◽  
Northern Analysis ◽  
Moderate Increase ◽  
Flow Rates ◽  
Rat Hearts ◽  
Heart Perfusion ◽  
Paired Control ◽  
Working Heart

We examined the expression of the mRNAs encoding for the inducible heat shock protein (HSP) 71 and the constitutively synthesized HSP73 in control and 24-h post-heat-shocked (post-HS) hearts during isolated working heart perfusion. Paired control and 24-h post-HS rat hearts were perfused in the working heart mode for 1, 2, 3, or 4 h. Aortic and coronary flow rates and heart rates were not different between the control and 24-h post-HS hearts during the perfusion periods. After perfusion, total RN A was extracted and separated by gel electrophoresis. RNA was blotted to membranes, subsequently probed with 32P-labelled cDNA probes for HSP71 and HSP73 transcripts, and autoradiographed. Control hearts showed a sharp increase in transcripts for HSP71 and a more moderate increase in transcripts for HSP73 accumulation during perfusion. However, the increase in HSP71 and HSP73 transcripts in the HS hearts was markedly less than that in the control hearts. This suppression in gene expression in the HS hearts seems to suggest a negative control mechanism regulating transcription of mRNA encoding HSP71 and HSP73.Key words: mRNA, heart, Northern analysis, working heart perfusion, heat shock.

Cardioprotective effect of KR-33889, a novel PARP inhibitor, against oxidative stress-induced apoptosis in H9c2 cells and isolated rat hearts

2017 ◽  
Vol 40 (5) ◽  
pp. 640-654 ◽  
Author(s):  
Eun-Seok Park ◽  
Do-Hyun Kang ◽  
Jun Chul Kang ◽  
Yong Chang Jang ◽  
Min-Ju Lee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Parp Inhibitor ◽  
Cardioprotective Effect ◽  
H9c2 Cells ◽  
Rat Hearts ◽  
Induced Apoptosis ◽  
Isolated Rat

Expression and cellular distribution of heat-shock and nuclear oncogene proteins in rat hearts

1991 ◽  
Vol 261 (5) ◽  
pp. H1443-H1451 ◽  
Author(s):  
L. H. Snoeckx ◽  
F. Contard ◽  
J. L. Samuel ◽  
F. Marotte ◽  
L. Rappaport
Keyword(s):  
Heat Shock ◽  
Cardiac Development ◽  
Pressure Overload ◽  
Adult Rats ◽  
Coronary Smooth Muscle ◽  
Rat Hearts ◽  
Nonmuscle Cells ◽  
Ontogenic Development ◽  
Coronary Endothelium ◽  
Myocardial Muscle

An early, transient accumulation of mRNAs of the protooncogenes c-fos and c-myc and the heat-shock protein HSP70 has been described in hypertrophying rat hearts. It is unclear 1) in which cardiac cell type-these gene activations occur and 2) whether the corresponding proteins are translated. We studied protein expression in rat hearts during ontogenic development and under stress conditions associated with pressure overload with the use of immunofluorescent techniques. During cardiac development no HSP70 could be detected. c-Fos was expressed consistently after birth but only in coronary smooth muscle cells, and c-Myc was found exclusively in adult coronary endothelium and myocardial nonmuscle cells. In adult rats, HSP70 and, to a lesser extent, c-Fos were induced in myocardial muscle and some nonmuscle cells within 3 h following methohexital sodium anesthesia. A similar, more intense immunolabeling of these peptides was observed after thoracotomy and/or aortic stenosis. The coronary c-Fos and c-Myc labeling remained unchanged in these conditions. Thus the expression in cardiac muscle and nonmuscle cells of the three peptides differs and depends on different triggers.

scholarly journals The cardioprotective effect of dexmedetomidine on global ischaemia in isolated rat hearts

Resuscitation ◽  
2007 ◽  
Vol 74 (3) ◽  
pp. 538-545 ◽  
Author(s):  
Hisako Okada ◽  
Tadayoshi Kurita ◽  
Toshiaki Mochizuki ◽  
Koji Morita ◽  
Shigehito Sato
Keyword(s):  
Cardioprotective Effect ◽  
Global Ischaemia ◽  
Rat Hearts ◽  
Isolated Rat

Effect of Salidroside on Cardiac Functional Recovery

2013 ◽  
Vol 798-799 ◽  
pp. 1030-1032
Author(s):  
Yan Zhang ◽  
Zhong Hua Zheng ◽  
Yue Peng Wang ◽  
Guo Liang Peng ◽  
Liu Hang Wang
Keyword(s):  
Flow Rate ◽  
Functional Recovery ◽  
Rat Heart ◽  
Coronary Flow ◽  
Left Ventricular ◽  
Cardioprotective Effect ◽  
Decrease Rate ◽  
Rat Hearts ◽  
Developed Pressure ◽  
Isolated Rat

To investigate the cardioprotective effect of salidroside to rat heart subjected to 8-hour hypothermic storage and 2-hour normothermic reperfusion. Isolated rat hearts were perfused with Langendorff model; after 30 minutes of baseline, the hearts were arrested and stored by St. Thomas solution (STS) without (STS group) or with different concentration salidroside at 4 °C for 8 hours, then reperfused for 2 hours. Compared with STS group, both middle and high dosage in STS greatly improved the recovery of left ventricular developed pressure (LVDP), maximum LVDP increase and decrease rate (±dp/dt), coronary flow rate (CF). Our study demonstrated that the salidroside was beneficial to improving cardiac functional recovery.

scholarly journals Cardioprotective Effect of Benidipine on Cardiac Performance and Remodeling in Failing Rat Hearts

2008 ◽  
Vol 21 (2) ◽  
pp. 224-230 ◽  
Author(s):  
T. Ohno ◽  
N. Kobayashi ◽  
K. Yoshida ◽  
H. Fukushima ◽  
H. Matsuoka
Keyword(s):  
Cardiac Performance ◽  
Cardioprotective Effect ◽  
Rat Hearts
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