Heterotopic hepatocyte transplantation utilizing pancreatic islet cotransplantation for hepatotrophic stimulation: morphologic and morphometric evaluation

1999 ◽  
Vol 15 (3-4) ◽  
pp. 168-174 ◽  
Author(s):  
U. Kneser ◽  
P. M. Kaufmann ◽  
H. C. Fiegel ◽  
J. M. Pollok ◽  
D. Kluth ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Hepatocyte Transplantation ◽  
Morphometric Evaluation ◽  
Hepatotrophic Stimulation

Evaluation of methods of hepatotrophic stimulation in rat heterotopic hepatocyte transplantation using polymers

1999 ◽  
Vol 34 (7) ◽  
pp. 1118-1123 ◽  
Author(s):  
Peter-Matthias Kaufmann ◽  
Kaoru Sano ◽  
Shiro Uyama ◽  
Christopher K Breuer ◽  
Gregory M Organ ◽  
...  
Keyword(s):  
Hepatocyte Transplantation ◽  
Hepatotrophic Stimulation ◽  
Evaluation Of Methods

The Role of Hepatotrophic Stimulation in Heterotopic Hepatocyte Transplantation

1993 ◽  
Vol 331 ◽  
Author(s):  
P.-M. Kaufmann ◽  
S. Uyama ◽  
K. Sano ◽  
D. Mooney ◽  
J. P. Vacanti
Keyword(s):  
Dna Synthesis ◽  
Partial Hepatectomy ◽  
Portacaval Shunt ◽  
Hepatocyte Transplantation ◽  
Synthesis Rate ◽  
Cell Area ◽  
Split Liver ◽  
Group A ◽  
Hepatotrophic Stimulation ◽  
Brdu Staining

AbstractIn the future, the development of methods for transplantation of large hepatocyte numbers could provide an alternative to the established liver and split liver transplantation. The use of three dimensional prevascularized polyvinyl alcohol (PVA) scaffolds have allowed hepatocyte transplantation equivalent to a whole liver mass. This study was designed to determine if a portacaval-shunt (PCS) or a 70% hepatectomy (70% HE) enhanced engraftment and proliferation of transplanted hepatocytes in heterotopic locations.Male Lewis rats served as both donors and recipients, respectively. PVA-sponges were implanted as matrices for the transplanted hepatocytes. Recipient animals were divided into four groups. All groups received transplantation of 5×107 Hepatocytes (HCTx). In addition, group A received 70% HE, group B received a PCS plus 70% HE whereas group D received PCS. Group C as a control, received only HCTx. Quantitative morphometric analysis of hepatocyte area was performed on day 0, 3, 7 and 14 after transplantation. BrdU staining was performed to study DNA-Synthesis in the graft on day 3 and 14.PCS alone and in combination with a partial hepatectomy led to significantly greater cell area one week after transplantation compared to the partial hepatectomy alone. 70% HE resulted in significantly larger cell area than the controls. At two weeks after transplantation, these significant differences persisted with the exception of Group A and C results which were not significantly different. On day 3, BrdU staining revealed a significantly higher DNA synthesis rate in Groups A and B compared to Group C. On day 14, no statistically significant differences in levels of DNA synthesis could be observed.We conclude that hepatocytes can be successfully transplanted into PVA-devices. Engraftment and proliferation can be significantly enhanced by using portacaval shunt and partial hepatectomy as hepatotrophic stimulation.

Silver Staining of Pancreatic Islets as Revealed by Electron Microscopy

1967 ◽  
Vol 25 ◽  
pp. 44-45
Author(s):  
Kazuaki Misugi ◽  
Nobuko Misugi ◽  
Hiroshi Yamada
Keyword(s):  
Pancreatic Islet ◽  
Silver Staining ◽  
Islet Cells ◽  
Severe Hypoglycemia ◽  
Granular Cell ◽  
Electron Microscopic Level ◽  
Staining Reaction ◽  
Electron Microscopic ◽  
Pancreatic Islet Cell ◽  
D Cell

The authors had described the fine structure of a type of pancreatic islet cell, which appeared different from typical alpha and beta cells, and tentatively considered that this third type of granular cell probably represents the D cell (Figure 1).Since silver staining has been widely used to differentiate different types of pancreatic islet cells by light microscopy, an attempt to examine this staining reaction at the electron microscopic level was made.Material and Method: Surgically removed specimens from three infants who suffered from severe hypoglycemia were used. The specimens were fixed and preserved in 20% neutral formalin. Frozen sections, 30 to 40 micron thick, were prepared and they were stained by Bielschowsky's method as modified by Suzuki (2). The stained sections were examined under a microscope and islet tissues were isolated. They were fixed in 1% osmium tetroxide in phosphate buffer for one hour and embedded in Epon 812 following dehydration through a series of alcohols and propylene oxide.

APPLICATION DE LA DOUBLE ÉPREUVE D'HYPERGLYCÉMIE INTRAVEINEUSE À L'ÉTUDE DU DIABÈTE

1960 ◽  
Vol XXXIII (II) ◽  
pp. 157-167
Author(s):  
T. Rodari ◽  
G. Specchia
Keyword(s):  
Glucose Tolerance ◽  
Pancreatic Islet ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Intravenous Glucose Tolerance Test ◽  
Intravenous Glucose ◽  
Intravenous Glucose Tolerance ◽  
Glucose Assimilation

ABSTRACT The double intravenous glucose tolerance test does not modify the assimilation coefficient in normal and thin diabetic subjects. On the contrary, in fat diabetic subjects the second coefficient of assimilation increases significantly, but not the first one. From these researches it is evident that the valuation of glucose assimilation by double venous hyperglycaemic test indicates the functional behaviour of the pancreas in different diabetic states. The interpretation of this behaviour of pancreatic islet response to the double venous hyperglycaemic test is discussed.

Diazoxide pretreatment improves pancreatic islet survival in vitro and functionality in vivo

2018 ◽  
Author(s):  
Michiel Nijhoff ◽  
Francoise Carlotti ◽  
Marten Engelse ◽  
Koning Eelco de
Keyword(s):  
Pancreatic Islet ◽  
Islet Survival

Correlations between Serum 25-Hydroxyvitamin D and Pancreatic Islet Function in Type 2 Diabetics

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 779-P
Author(s):  
NAN LI ◽  
MING CHEN ◽  
JIAQI DONG ◽  
SHAOLING YANG ◽  
LU XU ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Islet Function ◽  
Hydroxyvitamin D ◽  
Type 2 Diabetics ◽  
25 Hydroxyvitamin D
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