Soil DNA extraction and assessment of the fate of Mycobacterium chlorophenolicum strain PCP-1 in different soils by 16S ribosomal RNA gene sequence based most-probable-number PCR and immunofluorescence

1997 ◽  
Vol 24 (2) ◽  
pp. 188-195 ◽  
Author(s):  
J. D. van Elsas ◽  
V. M�ntynen ◽  
A. C. Wolters
Keyword(s):  
Dna Extraction ◽  
Ribosomal Rna ◽  
Gene Sequence ◽  
Most Probable Number ◽  
Soil Dna ◽  
Probable Number ◽  
Ribosomal Rna Gene ◽  
16S Ribosomal Rna Gene ◽  
Mycobacterium Chlorophenolicum ◽  
Different Soils

16S Ribosomal RNA Gene Sequence and Phylogeny of ToxicMicrocystissp. (Cyanobacteria)

DNA Sequence ◽  
1994 ◽  
Vol 4 (5) ◽  
pp. 333-337 ◽  
Author(s):  
Brett A. Neilan ◽  
Peter T. Cox ◽  
Peter R. Hawkins ◽  
Amanda E. Goodman
Keyword(s):  
Ribosomal Rna ◽  
Gene Sequence ◽  
16S Ribosomal Rna ◽  
Ribosomal Rna Gene ◽  
16S Ribosomal Rna Gene

scholarly journals Definitive Assignment by Multigenome Analysis of the Gammaproteobacterial Genus Thermithiobacillus to the Class Acidithiobacillia

2014 ◽  
Vol 63 (2) ◽  
pp. 245-247 ◽  
Author(s):  
COREY M. HUDSON ◽  
KELLY P. WILLIAMS ◽  
DONOVAN P. KELLY
Keyword(s):  
Phylogenetic Analysis ◽  
Genome Sequence ◽  
Ribosomal Rna ◽  
Gene Sequence ◽  
Nearest Neighbour ◽  
Systematic Bacteriology ◽  
Ribosomal Rna Gene ◽  
16S Ribosomal Rna Gene ◽  
Thermithiobacillus Tepidarius ◽  
Sequence Similarities

The class Acidithiobacillia was established using multiprotein phylogenetic analysis of all the available genomes of the genus Acidithiobacillus (comprising Family I, the Acidithiobacillaceae, of the Acidithiobacillales, the order created for Bergey's Manual of Systematic Bacteriology), and for representative genomes of all available bacterial orders. The Acidithiobacillales contain a second family, the Thermithiobacillaceae, represented by Thermithiobacillus tepidarius, and created on the basis of nearest neighbour 16S ribosomal RNA gene sequence similarities. This could not be included in the original multiprotein analysis as no genome sequence for Thermithio bacillus was available. The publication of the genome sequence of Thermithiobacillus tepidarius in 2013 has enabled phylogenetic assessment of this organism by comparative multigenome analysis. This shows definitively that Thermithiobacillus is a member of the class Acidithiobacillia, distinct from the Acidithiobacillus genus, and confirms it to represent a second family within the Acidithiobacillia.

scholarly journals Identification of probiotic lactobacilli used for animal feeds on the basis of 16S ribosomal RNA gene sequence

2008 ◽  
Vol 52 (11) ◽  
pp. 559-563 ◽  
Author(s):  
Wataru Higuchi ◽  
Mineo Muramatsu ◽  
Soshi Dohmae ◽  
Tomomi Takano ◽  
Hirokazu Isobe ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Gene Sequence ◽  
16S Ribosomal Rna ◽  
Ribosomal Rna Gene ◽  
Animal Feeds ◽  
16S Ribosomal Rna Gene ◽  
Probiotic Lactobacilli

scholarly journals Characterization and identification of three thermophilic Bacillus strain isolated from Domas Crater, Mt. Tangkuban Perahu, Indonesia

2020 ◽  
Vol 21 (8) ◽  
Author(s):  
RATU SAFITRI ◽  
DELICIA PUTRI KUSUMAWARDHANI ◽  
ANNISA ANNISA ◽  
Ruhyat Partasasmita ◽  
SINTA ASHARINA ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Gene Sequence ◽  
Hot Spring ◽  
16S Ribosomal Rna ◽  
Thermostable Enzymes ◽  
Thermophilic Bacillus ◽  
Ribosomal Rna Gene ◽  
16S Ribosomal Rna Gene ◽  
Plant Growth Promoting ◽  
Taxonomic Groups

Abstract. Safitri R, Kusumawardhani PD, Annisa, Partasasmita R, Asharina S, Maskoen AM. 2020. Characterization and identification of three thermophilic Bacillus strains isolated from Domas Crater, Mt. Tangkuban Perahu, Indonesia. Biodiversitas 21: 3444-3453. A community of thermophiles within the hot spring of Domas Crater, Mount Tangkuban West Java, has been cultivated and identified based on the 16S ribosomal RNA gene sequence. The hot spring has a temperature of 45°C-76 °C, pH 1-2, the isolate has been cultivated in Thermus medium at a temperature of 70 and pH 6. The three isolated strains were L61A, TS61A, and D41A identified based on the 16S ribosomal RNA gene sequence refer to the GenBank database and all of them belong to the Phylum Firmicutes, which are clustered within the taxonomic groups of Bacillus, the Bacillus, and Geobacillus genera. Nucleotide sequences were compared for homology with BLASTN search at the NCBI Web-site revealed that strains (L61A) had 99.93% similarity with Geobacillus uzenensis. TS61A strain has a 99.24% similarity with Bacillus wiedmannii. Whereas D41A has 99.79% similarity with Bacillus paramycoides. The characterization also includes the phenotypic and biochemical of strains. The bacillus genera were known as a source of thermostable enzymes, plant growth-promoting, probiotics, decomposers of various polysaccharide materials, and bioremediation.

Sign in / Sign up

Export Citation Format

Share Document