The central role of microbial activity for iron acquisition in maize and sunflower

2000 ◽  
Vol 30 (5-6) ◽  
pp. 433-439 ◽  
Author(s):  
J. Masalha ◽  
H. Kosegarten ◽  
Ö. Elmaci ◽  
K. Mengel
Keyword(s):  
Microbial Activity ◽  
Iron Acquisition

scholarly journals Physiological and interactomic analysis reveals versatile functions of Arabidopsis 14-3-3 quadruple mutants in response to Fe deficiency

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jing Gao ◽  
Paula J. M. van Kleeff ◽  
Ka Wan Li ◽  
Albertus H. de Boer
Keyword(s):  
Iron Acquisition ◽  
Tca Cycle ◽  
Utilization Efficiency ◽  
Fe Deficiency ◽  
Transcriptional Level ◽  
Functional Aspects ◽  
Clear Explanation ◽  
Physiological Analysis ◽  
Proper Operation

AbstractTo date, few phenotypes have been described for Arabidopsis 14-3-3 mutants or the phenotypes showing the role of 14-3-3 in plant responding to abiotic stress. Although one member of the 14-3-3 protein family (14-3-3 omicron) was shown to be involved in the proper operation of Fe acquisition mechanisms at physiological and gene expression levels in Arabidopsis thaliana, it remains to be explored whether other members play a role in regulating iron acquisition. To more directly and effectively observe whether members of 14-3-3 non-epsilon group have a function in Fe-deficiency adaptation, three higher order quadruple KOs, kappa/lambda/phi/chi (klpc), kappa/lambda/upsilon/nu(klun), and upsilon/nu/phi/chi (unpc) were generated and studied for physiological analysis in this study. The analysis of iron-utilization efficiency, root phenotyping, and transcriptional level of Fe-responsive genes suggested that the mutant with kl background showed different phenotypes from Wt when plants suffered Fe starved, while these phenotypes were absent in the unpc mutant. Moreover, the absence of the four 14-3-3 isoforms in the klun mutant has a clear impact on the 14-3-3 interactome upon Fe deficiency. Dynamics of 14-3-3-client interactions analysis showed that 27 and 17 proteins differentially interacted with 14-3-3 in Wt and klun roots caused by Fe deficiency, respectively. Many of these Fe responsive proteins have a role in glycolysis, oxidative phosphorylation and TCA cycle, the FoF1-synthase and in the cysteine/methionine synthesis. A clear explanation for the observed phenotypes awaits a more detailed analysis of the functional aspects of 14-3-3 binding to the target proteins identified in this study.

scholarly journals Petrobactin, a siderophore produced by Alteromonas, mediates community iron acquisition in the global ocean

2021 ◽  
Author(s):  
Lauren E. Manck ◽  
Jiwoon Park ◽  
Benjamin J. Tully ◽  
Alfonso M. Poire ◽  
Randelle M. Bundy ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Iron Acquisition ◽  
Biogeochemical Cycling ◽  
Iron Bioavailability ◽  
Siderophore Production ◽  
Global Ocean ◽  
The North ◽  
The North Pacific ◽  
Alteromonas Macleodii

AbstractIt is now widely accepted that siderophores play a role in marine iron biogeochemical cycling. However, the mechanisms by which siderophores affect the availability of iron from specific sources and the resulting significance of these processes on iron biogeochemical cycling as a whole have remained largely untested. In this study, we develop a model system for testing the effects of siderophore production on iron bioavailability using the marine copiotroph Alteromonas macleodii ATCC 27126. Through the generation of the knockout cell line ΔasbB::kmr, which lacks siderophore biosynthetic capabilities, we demonstrate that the production of the siderophore petrobactin enables the acquisition of iron from mineral sources and weaker iron-ligand complexes. Notably, the utilization of lithogenic iron, such as that from atmospheric dust, indicates a significant role for siderophores in the incorporation of new iron into marine systems. We have also detected petrobactin, a photoreactive siderophore, directly from seawater in the mid-latitudes of the North Pacific and have identified the biosynthetic pathway for petrobactin in bacterial metagenome-assembled genomes widely distributed across the global ocean. Together, these results improve our mechanistic understanding of the role of siderophore production in iron biogeochemical cycling in the marine environment wherein iron speciation, bioavailability, and residence time can be directly influenced by microbial activities.

scholarly journals Rare microbes from diverse Earth biomes dominate community activity

2019 ◽  
Author(s):  
Rohan Sachdeva ◽  
Barbara J. Campbell ◽  
John F. Heidelberg
Keyword(s):  
Community Structure ◽  
Microbial Activity ◽  
Ecosystem Function ◽  
Total Activity ◽  
Reaction Rates ◽  
Community Activity ◽  
Crucial Component ◽  
Tightly Coupled ◽  
The Relationship

AbstractMicrobes are the Earth’s most numerous organisms and are instrumental in driving major global biological and chemical processes. Microbial activity is a crucial component of all ecosystems, as microbes have the potential to control any major biochemical process. In recent years, considerable strides have been made in describing the community structure,i.e. diversity and abundance, of microbes from the Earth’s major biomes. In virtually all environments studied, a few highly abundant taxa dominate the structure of microbial communities. Still, microbial diversity is high and is concentrated in the less abundant, or rare, fractions of the community,i.e. the “long tail” of the abundance distribution. The relationship between microbial community structure and activity, specifically the role of rare microbes, and its connection to ecosystem function, is not fully understood. We analyzed 12.3 million metagenomic and metatranscriptomic sequence assemblies and their genes from environmental, human, and engineered microbiomes, and show that microbial activity is dominated by rare microbes (96% of total activity) across all measured biomes. Further, rare microbial activity was comprised of traits that are fundamental to ecosystem and organismal health,e.g. biogeochemical cycling and infectious disease. The activity of rare microbes was also tightly coupled to temperature, revealing a link between basic biological processes,e.g. reaction rates, and community activity. Our study provides a broadly applicable and predictable paradigm that implicates rare microbes as the main microbial drivers of ecosystem function and organismal health.

scholarly journals Specific Effect of Trace Metals on Marine Heterotrophic Microbial Activity and Diversity: Key Role of Iron and Zinc and Hydrocarbon-Degrading Bacteria

2018 ◽  
Vol 9 ◽  
Author(s):  
Federico Baltar ◽  
Andrés Gutiérrez-Rodríguez ◽  
Moana Meyer ◽  
Isadora Skudelny ◽  
Sylvia Sander ◽  
...  
Keyword(s):  
Trace Metals ◽  
Microbial Activity ◽  
Specific Effect ◽  
Degrading Bacteria ◽  
Iron And Zinc

Role of lung iron in determining the bacterial and host struggle in cystic fibrosis

2009 ◽  
Vol 297 (5) ◽  
pp. L795-L802 ◽  
Author(s):  
D. W. Reid ◽  
G. J. Anderson ◽  
I. L. Lamont
Keyword(s):  
Cystic Fibrosis ◽  
Iron Homeostasis ◽  
Iron Acquisition ◽  
Genetic Disorder ◽  
Bacterial Species ◽  
System Disease ◽  
Liver And Pancreas ◽  
Pseudomonas Aeruginosa Infection ◽  
The Relationship

Cystic fibrosis (CF) is the most common lethal genetic disorder in Caucasian populations. It is a multiorgan system disease that affects the lungs, gastrointestinal tract, liver, and pancreas. The majority of morbidity and mortality in CF relates to chronic airway infection with a variety of bacterial species, commencing in very early infancy, which results in lung destruction and ultimately organ failure ( 41 , 43 ). This review focuses on iron homeostasis in the CF lung and its role in determining the success and chronicity of Pseudomonas aeruginosa infection. There have been previous excellent reviews regarding iron metabolism in the lower respiratory tract and mechanisms of P. aeruginosa iron acquisition, and we direct readers to these articles for further background reading ( 31 , 53 , 58 , 77 , 96 ). In this review, we have brought the “two sides of the coin” together to provide a holistic overview of the relationship between host and bacterial iron homeostasis and put this information into the context of current understanding on infection in the CF lung.

scholarly journals The role of microorganisms in the formation of a stalactite in Botovskaya Cave, Siberia – paleoenvironmental implications

2013 ◽  
Vol 10 (9) ◽  
pp. 6115-6130 ◽  
Author(s):  
M. Pacton ◽  
S. F. M. Breitenbach ◽  
F. A. Lechleitner ◽  
A. Vaks ◽  
C. Rollion-Bard ◽  
...  
Keyword(s):  
Microbial Activity ◽  
Manganese Oxides ◽  
Isotope Geochemistry ◽  
Isotopic Signature ◽  
Morphological Evidence ◽  
Calcite Deposition ◽  
Inorganic Precipitation ◽  
Co2 Degassing ◽  
Iron And Manganese

Abstract. Calcitic speleothems in caves can form through abiogenic or biogenic processes, or through a combination of both. Many issues conspire to make the assessment of biogenicity difficult, especially when focusing on old speleothem deposits. This study reports on a multiproxy analysis of a Siberian stalactite, combining high-resolution microscopy, isotope geochemistry and microbially enhanced mineral precipitation laboratory experiments. The contact between growth layers in a stalactite exhibits a biogenic isotopic signature; coupled with morphological evidence, this supports a microbial origin of calcite crystals. SIMS δ13C data suggest that microbially mediated speleothem formation occurred repeatedly at short intervals before abiotic precipitation took over. The studied stalactite also contains iron and manganese oxides that have been mediated by microbial activity through extracellular polymeric substance (EPS)-influenced organomineralization processes. The latter reflect paleoenvironmental changes that occurred more than 500 000 yr ago, possibly related to the presence of a peat bog above the cave at that time. Microbial activity can initiate calcite deposition in the aphotic zone of caves before inorganic precipitation of speleothem carbonates. This study highlights the importance of microbially induced fractionation that can result in large negative δ13C excursions. The microscale biogeochemical processes imply that microbial activity has only negligible effects on the bulk δ13C signature in speleothems, which is more strongly affected by CO2 degassing and the host rock signature.

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