Inputs to the Active Indole-3-Acetic Acid Pool: De Novo Synthesis, Conjugate Hydrolysis, and Indole-3-Butyric Acid b-Oxidation

2001 ◽  
Vol 20 (3) ◽  
pp. 198-216 ◽  
Author(s):  
Bonnie Bartel ◽  
Sherry LeClere ◽  
Monica Magidin ◽  
Bethany K. Zolman
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
De Novo ◽  
De Novo Synthesis ◽  
Acid Pool ◽  
Indole 3 Acetic Acid ◽  
Conjugate Hydrolysis

scholarly journals In Vitro Multiple Bud Formation by 20-year-old Western Larch Buds and Stems

HortScience ◽  
1990 ◽  
Vol 25 (1) ◽  
pp. 114-116 ◽  
Author(s):  
E.E. Chesick ◽  
D.E. Bilderback ◽  
G.M. Blake
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
De Novo ◽  
Growing Season ◽  
Larix Occidentalis ◽  
Western Larch ◽  
Bud Formation ◽  
Multiple Buds ◽  
Indole 3 Acetic Acid

Vegetative long-shoot buds, greenwood stems, and immature needles of 20-year-old western larch (Larix occidentalis Nutt.) were cultured to induce multiple bud formation. Explants were collected year-round and cultured on a modified Schenk and Hildebrandt (SH) medium containing 6-benzyladenine (BA) at 0, 1, 5, 10, 50, or 100 μm. Multiple buds were produced on buds and stems with terminal meristems, but not on needles or stem sections. The induction of de novo buds and development of axillary buds required BA at 1 to 10 μm; higher concentrations of BA were less effective. More explants formed multiple buds on SH than on modified Murashige and Skoog (MS) media. Multiple buds formed on more buds and stems excised during the growing season than from dormant buds. Buds cultured on media containing gibberellin died within 6 weeks; auxin caused bud elongation but no multiple buds formed. Chemical names used: N-[(trichloromethyl)thio]-4-cyclohexene-1,2-dicarboximide (captan); 6-benzyladenine (BA); 1H-indole-3-butyric acid (IBA); 1H-indole-3-acetic acid (IAA); gibberellin (GA4+7).

scholarly journals In Vitro Culture of Heuchera villosa ‘Caramel’

HortScience ◽  
2017 ◽  
Vol 52 (4) ◽  
pp. 622-624 ◽  
Author(s):  
Hua Q. Zhao ◽  
Qing H. He ◽  
Li L. Song ◽  
Mei F. Hou ◽  
Zhi G. Zhang
Keyword(s):  
Acetic Acid ◽  
In Vitro Culture ◽  
Butyric Acid ◽  
Shoot Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Induction Rate ◽  
Regenerated Plantlets ◽  
Indole 3 Acetic Acid

The procedure for Heuchera villosa ‘Caramel’ propagation was investigated, which involves shoot regeneration, rooting of regenerated shoots, and acclimation of regenerated plantlets. Petioles, as explants, were cultured on MS medium supplemented with 1-naphthylacetic acid (NAA), benzylaminopurine (BA), thidiazuron (TDZ) and callus formed on all media. Shoots were observed to proliferate from callus on media with BA and NAA, whereas no shoots regenerated on media with TDZ and NAA. On media containing 0.5 or 1.0 mg·L−1 BA in combination with NAA, the regenerated shoots showed severe hyperhydricity, whereas on media containing 0.1 mg·L−1 BA in combination with NAA, the regenerated shoots grew normally. The highest shoot induction rate, 90.6%, was obtained on media containing 0.1 mg·L−1 BA and 0.01 mg·L−1 NAA. The effects of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and NAA on rooting of H. villosa ‘Caramel’ was explored. The highest rooting rate (95%) was obtained on 1/2 MS medium containing 0.2 mg·L−1 NAA. In the subsequent acclimation experiments, about 85% of rooted plantlets survived and grew normally.

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