Molecular characterization of radiation- and chemically induced mutations associated with neuromuscular tremors, runting, juvenile lethality, and sperm defects in jdf2 mice

1999 ◽  
Vol 10 (9) ◽  
pp. 870-878 ◽  
Author(s):  
Mitchell Walkowicz ◽  
Yonggang Ji ◽  
Xiaojia Ren ◽  
Bernhard Horsthemke ◽  
Liane B. Russell ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Induced Mutations ◽  
Chemically Induced

scholarly journals Construction and Characterization of Mutations within theKlebsiella mrkD1P Gene That Affect Binding to Collagen Type V

1999 ◽  
Vol 67 (4) ◽  
pp. 1672-1676 ◽  
Author(s):  
Tricia A. Sebghati ◽  
Steven Clegg
Keyword(s):  
Collagen Type ◽  
Gene Products ◽  
Induced Mutations ◽  
Binding Properties ◽  
Collagen Binding ◽  
Chemically Induced ◽  
Collagen Type V ◽  
Type V ◽  
Type 3

ABSTRACT The fimbria-associated MrkD1P protein mediates adherence of type 3 fimbriate strains of Klebsiella pneumoniae to collagen type V. Currently, three different MrkD adhesins have been described in Klebsiella species, and each possesses a distinctive binding pattern. Therefore, the binding abilities of mutants possessing defined mutations within themrkD 1P gene were examined in order to determine whether specific regions of the adhesin molecule were responsible for collagen binding. Both site-directed and chemically induced mutations were constructed within mrkD 1P, and the ability of the gene products to be incorporated into fimbrial appendages or bind to collagen was determined. Binding to type V collagen was not associated solely with one particular region of the MrkD1Pprotein, and two classes of nonadhesive mutants were isolated. In one class of mutants, the MrkD adhesin was not assembled into the fimbrial shaft, whereas in the second class of mutants, the adhesin was associated with fimbriae but did not bind to collagen. Both hemagglutinating and collagen-binding activities were associated with the MrkD1P molecule, since P pili and type 3 fimbriae carrying adhesive MrkD proteins exhibited identical binding properties.

scholarly journals Molecular characterization of the grape seeds extract’s effect against chemically induced liver cancer: In vivo and in vitro analyses

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Alaaeldin Ahmed Hamza ◽  
Gehan Hussein Heeba ◽  
Hanan Mohamed Elwy ◽  
Chandraprabha Murali ◽  
Raafat El-Awady ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Molecular Characterization ◽  
Grape Seeds ◽  
Chemically Induced

scholarly journals Generation and molecular characterization of CRISPR/Cas9-induced mutations in 63 immunity-associated genes in tomato reveals specificity and a range of gene modifications

2019 ◽  
Author(s):  
Ning Zhang ◽  
Holly M. Roberts ◽  
Joyce Van Eck ◽  
Gregory B. Martin
Keyword(s):  
Molecular Characterization ◽  
Gene Editing ◽  
Induced Mutations ◽  
Tomato Plants ◽  
Transgenic Tomato Plants ◽  
Target Sites ◽  
Targeted Gene Editing ◽  
Gene Alterations ◽  
New Mutations

AbstractThe CRISPR/Cas9 system is a powerful tool for targeted gene editing in many organisms including plants. However, most of the reported uses of CRISPR/Cas9 in plants have focused on modifying one or a few genes, and thus the overall specificity, types of mutations, and heritability of gene alterations remain unclear. Here we describe the molecular characterization of 361 T0 transgenic tomato plants that were generated using CRISPR/Cas9 to induce mutations in 63 immunity-associated genes. Among the T0 transformed plants, 245 carried mutations (68%), with 20% of those plants being homozygous for the mutation, 30% being heterozygous, 32% having two different mutations (biallelic) and 18% having multiple mutations (chimeric). The mutations were predominantly short insertions or deletions, with 87% of the affected sequences being smaller than 10 bp. The majority of 1 bp insertions were A (50%) or T (29%). The mutations from the T0 generation were stably transmitted to later generations, although new mutations were detected in some T1 plants. No mutations were detected in 18 potential off-target sites among 144 plants. Our study provides a broad and detailed view into the effectiveness of CRISPR/Cas9 for genome editing in an economically important plant species.

Molecular characterization of proton beam-induced mutations in soybean using genotyping-by-sequencing

2018 ◽  
Vol 293 (5) ◽  
pp. 1169-1180 ◽  
Author(s):  
Woon Ji Kim ◽  
Jaihyunk Ryu ◽  
Juhyun Im ◽  
Sang Hun Kim ◽  
Si-Yong Kang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Proton Beam ◽  
Genotyping By Sequencing ◽  
Induced Mutations
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