PCR in situ followed by microdissection allows whole chromosome painting probes to be made from single microdissected chromosomes

1999 ◽  
Vol 10 (6) ◽  
pp. 628-631 ◽  
Author(s):  
Allen T. Christian ◽  
Holly E. Garcia ◽  
James D. Tucker
Keyword(s):  
Chromosome Painting ◽  
Painting Probes ◽  
Pcr In Situ ◽  
Whole Chromosome Painting

scholarly journals Identification of satellited markers by microdissection and fluorescence in situ hybridization: a clinical case of isodicentric chromosome 22

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Natalya A. Lemskaya ◽  
Svetlana A. Romanenko ◽  
Yulia V. Maksimova ◽  
Asia R. Shorina ◽  
Dmitry V. Yudkin
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosome Painting ◽  
Chromosome Rearrangement ◽  
Marker Chromosome ◽  
Abnormal Development ◽  
Fish Analysis ◽  
Painting Probes ◽  
Whole Chromosome Painting

Abstract Background The presence of small supernumerary marker chromosomes (sSMCs) in a karyotype leads to diagnostic questions because the resulting extra material may cause abnormal development depending on the origin of the duplication/triplication. Because SMCs are so small, their origin cannot be determined by conventional cytogenetic techniques, and new molecular cytogenetic methods are necessary. Here, we applied a target approach to chromosome rearrangement analysis by isolating a chromosome of interest via microdissection and using it in fluorescence in situ hybridization (FISH) as a probe in combination with whole-chromosome painting probes. This approach allows to identify origins of both the euchromatin and repeat-rich regions of a marker. Case presentation We report a case of an adult male with congenital atresia of the rectum and anus, anotia, and atresia of the external auditory canal along with hearing loss. Karyotyping and FISH analysis with whole-chromosome painting probes of acrocentric chromosomes and the constructed microdissection library of the marker chromosome reliably identified an additional chromosome in some metaphases: mos 47,XY,+idic(22)(q11.2)[14]/46,XY [23]. Conclusion We propose to use whole-chromosome libraries and microdissected chromosomes in FISH to identify SMCs enriched with repeated sequences. We show that the methodology is successful in identifying the composition of a satellited marker chromosome.

scholarly journals Generation of a whole chromosome painting probe from monochromosomal hybrid cells by the alu-polymerase chain reaction

2007 ◽  
Vol 59 (2) ◽  
pp. 89-95 ◽  
Author(s):  
Danijela Drakulic ◽  
Gordana Nikcevic ◽  
Vesna Djordjevic ◽  
Milena Stevanovic
Keyword(s):  
Chromosome Painting ◽  
Fluorescent In Situ Hybridization ◽  
Chromosomal Rearrangements ◽  
Painting Probe ◽  
Complex Chromosomal Rearrangements ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Complex Chromosome ◽  
Whole Chromosome Painting

Fluorescent in situ hybridization (FISH) has become a widespread technique applicable in basic science and diagnostics. Chromosome painting represents a special application of FISH that has found increasing use in identification of complex chromosome rearrangements. Here we present a version of the Alu-PCR method modified to generate a whole chromosome painting probe (WCP) for human chromosome 19 using monochromosomal cell hybrids. In setting up conditions for this method, we established a cheap and fast approach to generation of WCPs for other human chromosomes that could be particularly useful for unambiguous identification of complex chromosomal rearrangements associated with cancer. .

Different distributions of homologous chromosomes in adult human Sertoli cells and in lymphocytes signify nuclear differentiation

1996 ◽  
Vol 109 (4) ◽  
pp. 773-776 ◽  
Author(s):  
A.C. Chandley ◽  
R.M. Speed ◽  
A.R. Leitch
Keyword(s):  
Sertoli Cells ◽  
Cell Types ◽  
Fish Analysis ◽  
Cell Nuclei ◽  
Interphase Nuclei ◽  
Homologous Chromosomes ◽  
Blood Lymphocytes ◽  
Painting Probes ◽  
Whole Chromosome Painting

Using whole chromosome painting probes for human chromosomes 3,7,8,13,17 and 21 and X and the probe pHY2.1 for the Y chromosome coupled with fluorescent in situ hybridization (FISH) analysis, the distribution of chromosomes is reported in nuclei of Sertoli cells of the adult testis and in stimulated blood lymphocytes. The distribution of chromosomes in the two cell types is significantly different. A strong tendency for each pair of homologues to pair is inferred from the observation of only a single detectable signal in the majority of Sertoli cell nuclei. The sex chromosomes, by contrast, give two clearly separated signals. Interphase nuclei in dividing blood lymphocytes, analysed as controls, also show mainly two separated signals for all non-acrocentric autosomal pairs, but acrocentric pairs no. 13 and 21 show some tendency to associate, probably reflecting satellite association.

scholarly journals Introducing the Bird Chromosome Database: An Overview of Cytogenetic Studies in Birds

2020 ◽  
Vol 160 (4) ◽  
pp. 199-205 ◽  
Author(s):  
Tiago M. Degrandi ◽  
Suziane A. Barcellos ◽  
Alice L. Costa ◽  
Analía D.V. Garnero ◽  
Iris Hass ◽  
...  
Keyword(s):  
Chromosome Numbers ◽  
Chromosome Painting ◽  
Bird Species ◽  
Extensive Literature ◽  
Phylogenetic Studies ◽  
Cytogenetic Studies ◽  
History Of ◽  
Intrachromosomal Rearrangements ◽  
Painting Probes ◽  
Whole Chromosome Painting

Bird chromosomes, which have been investigated scientifically for more than a century, present a number of unique features. In general, bird karyotypes have a high diploid number (2n) of typically around 80 chromosomes that are divided into macro- and microchromosomes. In recent decades, FISH studies using whole chromosome painting probes have shown that the macrochromosomes evolved through both inter- and intrachromosomal rearrangements. However, chromosome painting data are available for only a few bird species, which hinders a more systematic approach to the understanding of the evolutionary history of the enigmatic bird karyotype. Thus, we decided to create an innovative database through compilation of the cytogenetic data available for birds, including chromosome numbers and the results of chromosome painting with chicken (Gallus gallus) probes. The data were obtained through an extensive literature review, which focused on cytogenetic studies published up to 2019. In the first version of the “Bird Chromosome Database (BCD)” (https://sites.unipampa.edu.br/birdchromosomedatabase) we have compiled data on the chromosome numbers of 1,067 bird species and chromosome painting data on 96 species. We found considerable variation in the diploid numbers, which ranged from 40 to 142, although most (around 50%) of the species studied up to now have between 78 and 82 chromosomes. Despite its importance for cytogenetic research, chromosome painting has been applied to less than 1% of all bird species. The BCD will enable researchers to identify the main knowledge gaps in bird cytogenetics, including the most under-sampled groups, and make inferences on chromosomal homologies in phylogenetic studies.

scholarly journals Comparative Karyotype of Pig and Cattle Using Whole Chromosome Painting Probes

Hereditas ◽  
2004 ◽  
Vol 128 (3) ◽  
pp. 257-263 ◽  
Author(s):  
A. Schmitz ◽  
A. Oustry ◽  
D. Vaiman ◽  
B. Chaput ◽  
G. Frelat ◽  
...  
Keyword(s):  
Chromosome Painting ◽  
Painting Probes ◽  
Whole Chromosome Painting

Detection of equine X chromosome mosaicism in a mare using an equine X whole chromosome painting probe (WCPP) — A case report

2007 ◽  
Vol 55 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Monika Bugno ◽  
Ewa Słota ◽  
Aldona Pieńkowska-Schelling ◽  
C. Schelling
Keyword(s):  
Case Report ◽  
X Chromosome ◽  
Blood Lymphocyte ◽  
Chromosome Painting ◽  
Lymphocyte Culture ◽  
Painting Probe ◽  
Karyotype Formula ◽  
Mosaic Form ◽  
Whole Chromosome Painting

An infertile mare with hypoplastic ovaries was subjected to cytogenetic analysis. Fluorescence in situ hybridisation (FISH) using the equine X whole chromosome painting probe (WCPP) was carried out on a chromosome preparation obtained from blood lymphocyte culture. The number of analysed spreads was high (235) and in the X chromosome aneuploidy in mosaic form was diagnosed. The karyotype formula was 63,X / 64,XX / 65,XXX. The ratio of the three lines was 15%, 82% and 3%, respectively. The application of the FISH technique with WCPP is discussed.

Sign in / Sign up

Export Citation Format

Share Document