Hormone-sensitive lipase (Lipe): Sequence analysis of the 129Sv mouse Lipe gene

1997 ◽  
Vol 8 (2) ◽  
pp. 86-89 ◽  
Author(s):  
R. Sztrolovics ◽  
S. P. Wang ◽  
P. Lapierre ◽  
H. S. Chen ◽  
M -F. Robert ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Hormone Sensitive Lipase ◽  
Hormone Sensitive ◽  
129Sv Mouse

scholarly journals Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.

1981 ◽  
Vol 256 (12) ◽  
pp. 6311-6320
Author(s):  
G. Fredrikson ◽  
P. Strålfors ◽  
N.O. Nilsson ◽  
P. Belfrage
Keyword(s):  
Adipose Tissue ◽  
Hormone Sensitive Lipase ◽  
Hormone Sensitive ◽  
Rat Adipose Tissue

scholarly journals Testis Expression of Hormone-sensitive Lipase Is Conferred by a Specific Promoter That Contains Four Regions Binding Testicular Nuclear Proteins

1999 ◽  
Vol 274 (14) ◽  
pp. 9327-9334 ◽  
Author(s):  
Régis Blaise ◽  
Jacques Grober ◽  
Philippe Rouet ◽  
Geneviève Tavernier ◽  
Dominique Daegelen ◽  
...  
Keyword(s):  
Hormone Sensitive Lipase ◽  
Nuclear Proteins ◽  
Hormone Sensitive

Hormone-sensitive lipase: sequence, expression, and chromosomal localization to 19 cent-q13.3

Science ◽  
1988 ◽  
Vol 241 (4872) ◽  
pp. 1503-1506 ◽  
Author(s):  
C Holm ◽  
T. Kirchgessner ◽  
K. Svenson ◽  
G Fredrikson ◽  
S Nilsson ◽  
...  
Keyword(s):  
Chromosomal Localization ◽  
Hormone Sensitive Lipase ◽  
Hormone Sensitive

scholarly journals Effects of Physiological Hypercortisolemia on the Regulation of Lipolysis in Subcutaneous Adipose Tissue1

1998 ◽  
Vol 83 (2) ◽  
pp. 626-631 ◽  
Author(s):  
Jaswinder S. Samra ◽  
Mo L. Clark ◽  
Sandy M. Humphreys ◽  
Ian A. MacDonald ◽  
Peter A. Bannister ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Sodium Succinate ◽  
Hormone Sensitive Lipase ◽  
Whole Body ◽  
Constant Infusion ◽  
Nonesterified Fatty Acid ◽  
Significant Difference ◽  
Hormone Sensitive ◽  
Subcutaneous Adipose

Cortisol is known to increase whole body lipolysis, yet chronic hypercortisolemia results in increased fat mass. The main aim of the study was to explain these two apparently opposed observations by examining the acute effects of hypercortisolemia on lipolysis in subcutaneous adipose tissue and in the whole body. Six healthy subjects were studied on two occasions. On one occasion hydrocortisone sodium succinate was infused iv to induce hypercortisolemia (mean plasma cortisol concentrations, 1500 ± 100 vs. 335± 25 nmol/L; P < 0.001); on the other occasion (control study) no intervention was made. Lipolysis in the sc adipose tissue of the anterior abdominal wall was studied by measurement of arterio-venous differences, and lipolysis in the whole body was studied by constant infusion of[ 1,2,3-2H5]glycerol for measurement of the systemic glycerol appearance rate. Hypercortisolemia led to significantly increased arterialized plasma nonesterified fatty acid (NEFA; P < 0.01) and blood glycerol concentrations (P < 0.05), with an increase in systemic glycerol appearance (P < 0.05). However, in sc abdominal adipose tissue, hypercortisolemia decreased veno-arterialized differences for NEFA (P < 0.05) and reduced NEFA efflux (P < 0.05). This reduction was attributable to decreased intracellular lipolysis (P < 0.05), reflecting decreased hormone-sensitive lipase action in this adipose depot. Hypercortisolemia caused a reduction in arterialized plasma TAG concentrations (P < 0.05), but without a significant change in the local extraction of TAG (presumed to reflect the action of adipose tissue lipoprotein lipase). There was no significant difference in plasma insulin concentrations between the control and hypercortisolemia study. Site-specific regulation of the enzymes of intracellular lipolysis (hormone-sensitive lipase) and intravascular lipolysis (lipoprotein lipase) may explain the ability of acute cortisol treatment to increase systemic glycerol and NEFA appearance rates while chronically promoting net central fat deposition.

scholarly journals Sensitive assay for hormone-sensitive lipase using NBD-labeled monoacylglycerol to detect low activities in rat adipocytes

2005 ◽  
Vol 46 (3) ◽  
pp. 603-614 ◽  
Author(s):  
Stefan Petry ◽  
Yassine Ben Ali ◽  
Henri Chahinian ◽  
Holger Jordan ◽  
Horst Kleine ◽  
...  
Keyword(s):  
Hormone Sensitive Lipase ◽  
Sensitive Assay ◽  
Rat Adipocytes ◽  
Hormone Sensitive

scholarly journals Activation of hormone-sensitive lipase in extracts of adipose tissue

1970 ◽  
Vol 11 (5) ◽  
pp. 466-472
Author(s):  
SU-CHEN TSAI ◽  
PER BELFRAGE ◽  
MARTHA VAUGHAN
Keyword(s):  
Adipose Tissue ◽  
Hormone Sensitive Lipase ◽  
Hormone Sensitive

scholarly journals Expression of hormone-sensitive lipase and its regulation by adrenaline in skeletal muscle

1999 ◽  
Vol 340 (2) ◽  
pp. 459-465 ◽  
Author(s):  
Jozef LANGFORT ◽  
Thorkil PLOUG ◽  
Jacob IHLEMANN ◽  
Michele SALDO ◽  
Cecilia HOLM ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Kinase ◽  
Soleus Muscle ◽  
Lipase Activity ◽  
Glycogen Phosphorylase ◽  
Hormone Sensitive Lipase ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Hormone Sensitive ◽  
Neutral Lipase

The enzymic regulation of triacylglycerol breakdown in skeletal muscle is poorly understood. Western blotting of muscle fibres isolated by collagenase treatment or after freeze-drying demonstrated the presence of immunoreactive hormone-sensitive lipase (HSL), with the concentrations in soleus and diaphragm being more than four times the concentrations in extensor digitorum longus and epitrochlearis muscles. Neutral lipase activity determined under conditions optimal for HSL varied directly with immunoreactivity. Expressed relative to triacylglycerol content, neutral lipase activity in soleus muscle was about 10 times that in epididymal adipose tissue. In incubated soleus muscle, both neutral lipase activity against triacylglycerol (but not against a diacylglycerol analogue) and glycogen phosphorylase activity increased in response to adrenaline (epinephrine). The lipase activation was completely inhibited by anti-HSL antibody and by propranolol. The effect of adrenaline could be mimicked by incubation of crude supernatant from control muscle with the catalytic subunit of cAMP-dependent protein kinase, while no effect of the kinase subunit was seen with supernatant from adrenaline-treated muscle. The results indicate that HSL is present in skeletal muscle and is stimulated by adrenaline via β-adrenergic activation of cAMP-dependent protein kinase. The concentration of HSL is higher in oxidative than in glycolytic muscle, and the enzyme is activated in parallel with glycogen phosphorylase.

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