RAPD analysis of genetic variation and dispersal of the moss Bryum pseudotriquetrum from Southern Victoria Land, Antarctica

Polar Biology ◽  
1998 ◽  
Vol 20 (2) ◽  
pp. 121-126 ◽  
Author(s):  
M. L. Skotnicki ◽  
P. M. Selkirk ◽  
J. A. Ninham
Keyword(s):  
Genetic Variation ◽  
Rapd Analysis ◽  
Victoria Land ◽  
Bryum Pseudotriquetrum

scholarly journals Genetic variation among pathogens causing "Helminthosporium" diseases of rice, maize and wheat

2002 ◽  
Vol 27 (6) ◽  
pp. 639-643 ◽  
Author(s):  
RITA C. B. WEIKERT-OLIVEIRA ◽  
M. APARECIDA DE RESENDE ◽  
HENRIQUE M. VALÉRIO ◽  
RACHEL B. CALIGIORNE ◽  
EDILSON PAIVA
Keyword(s):  
Triticum Aestivum ◽  
Genetic Variation ◽  
Climatic Factors ◽  
Rapd Analysis ◽  
Its Region ◽  
Restriction Enzymes ◽  
Fungal Species ◽  
High Similarity ◽  
Pcr Rflp ◽  
Upgma Phenogram

Twenty isolates of four fungal species, agents of "Helminthosporium" diseases in cereals, were collected from different regions: nine Bipolarisoryzae isolated from rice (Oryza sativa), seven B.sorokiniana from wheat (Triticum aestivum), two B. maydis, and two Exserohilumturcicum from maize (Zea mays). The strains were compared by PCR-RFLP and RAPD analysis. Size polymorphism among the isolates in the ITS region comprising the 5.8 S rDNA indicated genetic differences among the isolates, while a UPGMA phenogram constructed after the digestion of this region with restriction enzymes showed inter- and intra-specific polymorphism. The RAPD profiles indicated an expressive level of polymorphism among different species, compared with a low level of polymorphism among isolates of the same species. A UPGMA phenogram grouped the isolates according to the species and their host plant. RAPD profiles did not reveal polymorphism that directly correlated climatic factors with geographic source of the isolates of B. sorokiniana, and B. oryzae. Teleomorphic species revealed high similarity with their correspondent anamorphs.

scholarly journals RAPD ANALYSIS OF GENETIC VARIATION IN NATURAL POPULATIONS OF AEGILOPS SP. FROM SOUTH ADRIATIC

AGROFOR ◽  
2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Miodrag DIMITRIJEVIĆ ◽  
Sofija PETROVIĆ ◽  
Borislav BANJAC ◽  
Goran BARAĆ
Keyword(s):  
Genetic Variation ◽  
Food Production ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Gene Diversity ◽  
Rapd Marker ◽  
Natural Populations ◽  
Genetic Structuring ◽  
Potential Source ◽  
Novel Approach

New challenges that food production is facing, requires novel approach inagricultural strategy. The scissors of growing demand for food and the limits of theEarth's resources are forcing plant breeders to run for the new borders, utilizing allthe available genetic variation in order to create fruitful and economically soundcultivars. Aegilops sp. (Poaceae) is a potential source of genetic variation for wheatimprovement. RAPD marker analysis was used in order to distinguish and evaluatedifferent genotypes of Aegilops sp. population samples from the collectiongathered during few years’ expeditions in South Adriatic, along the coastal, littoraland the inland parts of Montenegro. Ten randomly amplified polymorphic DNAmarkers (RAPDs) were tested: OPA-05, OPA-08, OPB-06, OPA-02, OPA-07,OPA-25, OPB-07, OPB-18, OPC-06, OPC-10 to examine genetic structuring on 18samples of 6 populations of different Aegilops sp. According to global AMOVA,75% of total gene diversity was attributable mostly to diversity within population(ΦPT =0.205 p=0.001), indicating that the groups of studied goat grass populationswere seemingly to differing genetically. In contrast, 25% of the variation camefrom variation among populations. According to PCoA, the distribution of 18 goatgrass accessions by Principal Coordinate Analysis shows 3 distinct groups. PCoaxis 1, PCo axis 2, and PCo axis 3 account for 20.8%, 18.2% and 14.1% of thevariation, respectively. The results showed that RAPD markers could be aconvenient tool for investigating genetic variation and for detecting geneticstructuring of populations. Genetic variability formed under natural selection wasentrenched.

scholarly journals Genetic diversity within and between broodstocks of the white shrimp Litopenaeus vannamei (Boone, 1931) (Decapoda, Penaeidae) and its implication for the gene pool conservation

2007 ◽  
Vol 67 (4 suppl) ◽  
pp. 939-943 ◽  
Author(s):  
PD. Freitas ◽  
MR. Calgaro ◽  
PM. Galetti Jr.
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Litopenaeus Vannamei ◽  
Gene Pool ◽  
Rapd Analysis ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Brazilian Coast ◽  
The Pacific ◽  
Improvement Programs

Genetic variation within and between fifteen closed broodstock lines of the Pacific white shrimp Litopenaeus vannamei, reared at different hatcheries in the Brazilian coast, was assessed by RAPD analysis. Fifty two polymorphic loci were identified when a set of five decamer primers was used in PCR. The genetic diversity analysis within lines evidenced genetic variation loss probably related to bottleneck effects and inbreeding. In addition, the genetic divergence values between the different samples appear to reflect the initial founder composition of such stocks, in some cases, sharing a common origin, suggesting a putative importance of interbreeding for the establishment of genetic improvement programs for these broodstocks. The genetic variation monitoring appears to be helpful to the gene pool conservation of this aquaculture species, mainly if considered its exotic status in Brazil and the current impossibility of new introduction of wild individuals.

RAPD Analysis of Genetic Variation Within and Among Populations of Turk's-Cap Lily (Lilium Martagon L.)

Hereditas ◽  
2004 ◽  
Vol 128 (3) ◽  
pp. 213-220 ◽  
Author(s):  
Helena A. Persson ◽  
Kjell Lundquist ◽  
Hilde Nybom
Keyword(s):  
Genetic Variation ◽  
Rapd Analysis

Detection of genetic variation in Sclerotinia homoeocarpa isolates using RAPD analysis

1997 ◽  
Vol 101 (5) ◽  
pp. 585-590 ◽  
Author(s):  
K. Raina ◽  
N. Jackson ◽  
J.M. Chandlee
Keyword(s):  
Genetic Variation ◽  
Rapd Analysis ◽  
Sclerotinia Homoeocarpa

scholarly journals Genetic variation estimated in three Shorea species by the RAPD analysis

1994 ◽  
Vol 69 (6) ◽  
pp. 713-718 ◽  
Author(s):  
Ko HARADA ◽  
Akira KINOSHITA ◽  
Nor Aini Ab SHUKOR ◽  
Hidenori TACHIDA ◽  
Tsuneyuki YAMAZAKI
Keyword(s):  
Genetic Variation ◽  
Rapd Analysis ◽  
Shorea Species

Genetic variation between ecotypic populations ofChloris roxburghianagrass detected through RAPD analysis

2005 ◽  
Vol 22 (2) ◽  
pp. 107-115 ◽  
Author(s):  
W Ngoyawu Mnene ◽  
J Hanson ◽  
WN Ekaya ◽  
JI Kinyamario ◽  
P Mweki ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Rapd Analysis

scholarly journals 469 PB 336 INTER- AND INTRA-LINE GENOTYPIC VARIATION OF U. S. COLLARD CULTIVARS AND LANDRACES DETERMINED BY RAPD ANALYSIS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 498d-498
Author(s):  
Mark W. Farnham
Keyword(s):  
Genetic Variation ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Genotypic Variation ◽  
Land Race ◽  
Commercial Cultivars ◽  
Polymorphic Bands ◽  
Line Analysis ◽  
Brassica Oleracea L

Collard (Brassica oleracea L. var. acephala) is an important vegetable the southeastern U. S. There are few (about 10) commercial cultivars, half being open-pollinating (OP) lines, the remainder more recent F1 hybrids. There is a potential untapped B. oleracea germplasm pool in the form of collard landraces perpetuated by southeastern gardeners and farmers. To determine the amount of genetic variation among cultivars and also whether landraces represent unique genotypes, ten cultivars and eight lines or landraces were evaluated using RAPD analysis. Decamer primers were used to amplify total genomic DNA and to differentiate collard lines and other B. oleracea crop cultivars. Additionally, individuals of an OP collard cultivar and a land-race were analyzed to evaluate intra-line variation. Virtually all primers detected polymorphic bands among lines although some identified considerably more variants. Intra-line analysis indicated that OP lines are genetically broad-based populations. Many unique RAPD markers were identified in landraces indicating that the lines represent unique genotypes and that further line collection is warranted.

scholarly journals Estimation of Genetic Variation in Grapes Based on RAPD and Isozyme Analysis

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 513E-513
Author(s):  
J. Lu ◽  
Z. Liu ◽  
Y. Zheng
Keyword(s):  
Genetic Variation ◽  
Genetic Relationship ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Isozyme Analysis ◽  
Similarity Coefficients ◽  
Taxonomic Information ◽  
Geographic Origins ◽  
Asiatic Species ◽  
Rapd Method

Genetic relationships among 42 grape accession of at least 15 species were estimated and compared using RAPD and isozyme techniques. These accessions were either hybrids or wild collections of the Asiatic species, the American species, the European grape (V. vinifera), and muscadine grape (V. rotundifolia). A total of 196 RAPD fragments were generated from twenty 10-mer primers. The pairwise similarities among the accession ranged from 0.46 to 0.94. A dendrogram was generated based on the RAPD similarity coefficients. Species/accessions were basically grouped together in accordance with their geographic origins. The similarities and dendrogram resulted from the RAPD analysis were consistent with the ones generated from the isozyme data, and also consistent with the known taxonomic information. This result suggest that the RAPD method, like isozyme, is an useful tool for studying grape genetic relationship/diversity and origination.

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