Agrobacterium tumefaciens AKE10-mediated transformation of an Asian pea pear, Pyrusbetulaefolia Bunge: host specificity of bacterial strains

2001 ◽  
Vol 20 (7) ◽  
pp. 622-628 ◽  
Author(s):  
J. Kaneyoshi ◽  
H. Wabiko ◽  
S. Kobayashi ◽  
T. Tsuchiya
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Host Specificity ◽  
Bacterial Strains

scholarly journals EFFICACY OF BIOCHEMICAL PREPARATIONS AND EXTRACT FROM Hypericum perforatum AGAINST BACTERIAL DISEASES

2019 ◽  
Vol 18 (3) ◽  
Author(s):  
Małgorzata Schollenberger ◽  
Sylwia Pudło ◽  
Elżbieta Paduch-Cichal ◽  
Ewa Mirzwa-Mróz
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
Hypericum Perforatum ◽  
Agar Plate ◽  
Nutrient Agar ◽  
Bacterial Strains ◽  
Bacterial Diseases ◽  
Nutrient Agar Medium

The biotechnical preparations: Biosept Active (based on a grapefruit extract) and BioZell (based on thyme oil) as well as Hypericum perforatum extract, streptomycin solution and fungicide Champion 50WP (active ingredient substance – e.i. 50% copper hydroxide) were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas ar- boricola pv. corylina. The screening was carried out in vitro on three media: Nutrient Agar (NA Difco), Pseudomonas Agar F (Merck) – analogue of King B and 523. In the experiments, the agar plate method was applied. There were no statistically significant differences in the effect of streptomycin and Champion 50WP on the growth inhibition of three bacteria strains for medium 523 and Nutrient Agar and of P. syringae pv. syringae and X. arboricola pv. corylina for medium King B. It was determined that the antibacterial activity of Biosept Active and BioZell biopreparations and H. perforatum extract against Agrobacterium tumefaciens (strain C58), Pseudomonas syringae pv. syringae (strain 760) and Xanthomonas arboricola pv. corylina (strain RIPF-x13) were dependent on the strain of pathogen as well as the growth medium used. According to the research results obtained, the Biosept Active preparation and H. perforatum extract demonstrated high bacteriostatic activity against three bacterial strains grown on the Nutrient Agar medium.

scholarly journals Chemical Composition and Antibacterial Activity of Essential oil from Salvia mukerjeei

2011 ◽  
Vol 6 (12) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Lalit Mohan ◽  
Anuradha Negi ◽  
Anand B. Melkani ◽  
Vasu Dev
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Agrobacterium Tumefaciens ◽  
Essential Oil ◽  
Volatile Oil ◽  
Erwinia Chrysanthemi ◽  
Bacterial Strains ◽  
Capillary Gc ◽  
Aerial Parts ◽  
Sesquiterpene Hydrocarbons

The composition of steam volatile oil from aerial parts of Salvia mukerjeei Bennet & Raizada (Lamiaceae) was analyzed by capillary GC and GCMS. The oil was rich in sesquiterpene hydrocarbons (67.3%). Among 71 identified constituents representing 91.7% of the oil, β-caryophyllene (28.7%), γ-muurolene (15.5%) and dehydro-aromadendrane (9.5%), were the principal constituents. The oil was tested against ten bacterial strains and was active against Enterococcus faecalis, Erwinia chrysanthemi and Agrobacterium tumefaciens.

scholarly journals 082 Infection and Transformation of Rhododendron by Agrobacterium tumefaciens Strain B6

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 455C-455 ◽  
Author(s):  
R.R. Tripepi ◽  
M.W. George ◽  
T. Sripo ◽  
S.A. Johnsen ◽  
A.B. Caplan
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetically Modified ◽  
Green Fluorescence Protein ◽  
Plant Genome ◽  
Wild Type ◽  
Bacterial Strains ◽  
Green Fluorescence ◽  
Physical Presence ◽  
Fluorescence Protein ◽  
Polymerase Chain

The objective of this study was to determine if selected strains of Agrobacterium could infect microshoots of Rhododendron catawbiense. Fifteen microshoot stems of R. catawbiense var. album `America', `Joe Paterno', and `Cunningham's White' were inoculated with two drops (about 25 μL) of wild type Agrobacterium tumefaciens strains C58 or B6 or with wild type A. rhizogenes strain E8/73. Five control shoots were inoculated with 1.2 mM KH2PO4 buffer. Microshoots were grown on woody plant medium (WPM) supplemented with 4.9 μM 2iP. Six weeks after inoculation galls that formed were excised from the microshoots and placed on WPM that lacked plant growth regulators but contained 300 mg·L-1 cefotaxime. In another study, these wild-type bacterial strains were genetically modified by inserting the pBINm-gfp5-ER plasmid, which contained genes coding for NPTII and green fluorescence protein (GFP), into the bacteria. These modified strains were inoculated on 15 stems of the three rhododendron cultivars and one variety. Calluses that formed were excised, placed on basal WPM with cefotaxime, and allowed to proliferate. Wild type C58 induced galls to form on `Joe Paterno', R.c. album, and `Cunningham's White' stems, whereas wild type B6 caused galls to form only on the latter two types of rhododendron. Wild-type E8/73 failed to induce gall formation on the rhododendrons. Only genetically modified B6 caused galls to form on only `Cunningham's White' microshoots (seven of 15 inoculated stems). Three of these galls fluoresced green under ultraviolet light. Physical presence of the NPTII and GFP genes in the plant genome was determined by polymerase chain reaction. This study demonstrated that R. catawbiense is susceptible to Agrobacterium infection, and this plant can be genetically transformed.

scholarly journals Bioactivity of Root Endophytic Freshwater HyphomycetesAnguillospora longissima(Sacc. & Syd.) Ingold

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
S. C. Sati ◽  
Lokendra Singh
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Agrobacterium Tumefaciens ◽  
Erwinia Chrysanthemi ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
Minimum Zone ◽  
Antimicrobial Assay ◽  
Antibacterial Potential

Anguillospora longissima, isolated from root as endophytic freshwater hyphomycetes, was evaluated for its bioactivity (antibacterial potential) against five bacterial strains, namely, Gram-positive (Bacillus subtilisMTCC 121) and Gram-negative (Agrobacterium tumefaciensMTCC 609,Escherichia coliMTCC 40,Erwinia chrysanthemum,andXanthomonas pseudomonas). Antimicrobial activity was assessed by measuring the zone of inhibition with preliminary and secondary antimicrobial assays. The applied fungus was found significant for all tested bacterial strains as showen by their zone of inhibition. In preliminary antimicrobial assay, maximum zone of inhibition was recorded against Gram-negative human pathogenic bacterial strainEscherichia coli(23 mm) followed byErwinia chrysanthemi(22 mm),Agrobacterium tumefaciens(21 mm), andXanthomonas phaseoli(21 mm), while minimum zone of inhibition was observed againstBacillus subtilis(20 mm). In secondary antimicrobial assay, the maximum zone of inhibition was recorded againstErwinia chrysanthemi(11 mm) followed byAgrobacterium tumefaciens(10 mm),Xanthomonas phaseoli(10 mm), andBacillus subtilis(9 mm) and minimum inhibition was found againstEscherichia coli(8 mm).

scholarly journals Identification and Characterization of a Pseudomonas mosselii strain and its Antibacterial Function against Agrobacterium tumefaciens

2021 ◽  
Vol 4 (2) ◽  
pp. 223-231
Author(s):  
Zhilong Wang ◽  
◽  
Wen Li ◽  
Jiaying Wang ◽  
Xintong Jiang ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Biochemical Characterization ◽  
Inhibition Zone ◽  
Tumor Formation ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Cherry Tree ◽  
Bacterial Colony ◽  
Crown Gall Disease

Agrobacterium tumefaciens is a gram negative bacterial that can infect a range of plants and result in root crown gall. A total number of 10328 bacterial strains were isolated from rhizosphere of cherry tree. One strain of LWB10 showed clear inhibition zone around the bacterial colony in YEB media inoculated with A. tumefaciens C58. Morphological, physiological, and biochemical characterization indicated that LWB10 belongs to member of the genus Pseudomonas. Results from the high-throughput matrix-assisted laser desorption/ionization biotypersmart system indicated that this strain had a score value of 2.247 relative to Pseudomonas mosselii. Also, phylogenetic analysis based on 16S rRNA gene sequence showed that strain LWB10 shared the highest similarity with Pseudomonas mosselii CIP 105259T. The antagonist strains also exhibit well in growth inhibition of other five A. tumefaciens strains. Coinoculation of LWB10 and plant pathogenic strain of A. tumefaciens CFCC1369 showed strongly inhibition of tumor formation in tomato stems. All the results demonstrated that the isolated strain is P. mosselii LWB10 and its antibacterial ability to A. tumefaciens may offer new way for management of crown gall disease in the future. Keywords: Agrobacterium tumefaciens, Pseudomonas mosselii, antagonist, biological control

scholarly journals Agrobacterium tumefaciens - associative microsymbionts of grain crop

2020 ◽  
Author(s):  
A. I. Yakubovskaya ◽  
I. A. Kameneva ◽  
T. N. Melnichuk ◽  
T. V. Matveeva ◽  
M. V. Gritchin ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Root Development ◽  
Genome Structure ◽  
Diazotrophic Bacteria ◽  
Bacterial Strains ◽  
Ti Plasmids ◽  
Grain Crop

The installed absence of Ti-plasmids in the genome structure of strains of diazotrophic bacteria Agrobacterium tumefaciens 32 and A. tumefaciens P3 was found, which characterizes the strains as non-pathogenic. Bacterization of grain seeds by associative bacterial strains stimulates root development by up to 40%.

scholarly journals Plant Transformation by Coinoculation with a Disarmed Agrobacterium tumefaciens Strain and an Escherichia coli Strain Carrying Mobilizable Transgenes

2003 ◽  
Vol 69 (11) ◽  
pp. 6731-6739 ◽  
Author(s):  
Katherine M. Pappas ◽  
Stephen C. Winans
Keyword(s):  
Escherichia Coli ◽  
Agrobacterium Tumefaciens ◽  
Plant Cells ◽  
Leaf Explants ◽  
Coli Strain ◽  
Broad Host Range ◽  
Transfer System ◽  
Bacterial Strains ◽  
Conjugation System ◽  
E Coli

ABSTRACT Transformation of Nicotiana tabacum leaf explants was attempted with Escherichia coli as a DNA donor either alone or in combination with Agrobacterium tumefaciens. We constructed E. coli donor strains harboring either the promiscuous IncP-type or IncN-type conjugal transfer system and second plasmids containing the respective origins of transfer and plant-selectable markers. Neither of these conjugation systems was able to stably transform plant cells at detectable levels, even when VirE2 was expressed in the donor cells. However, when an E. coli strain expressing the IncN-type conjugation system was coinoculated with a disarmed A. tumefaciens strain, plant tumors arose at high frequencies. This was caused by a two-step process in which the IncN transfer system mobilized the entire shuttle plasmid from E. coli to the disarmed A. tumefaciens strain, which in turn processed the T-DNA and transferred it to recipient plant cells. The mobilizable plasmid does not require a broad-host-range replication origin for this process to occur, thus reducing its size and genetic complexity. Tumorigenesis efficiency was further enhanced by incubation of the bacterial strains on medium optimized for bacterial conjugation prior to inoculation of leaf explants. These techniques circumvent the need to construct A. tumefaciens strains containing binary vectors and could simplify the creation of transgenic plants.

scholarly journals Phases of crown-gall transformation susceptible to hydroxyurea

2014 ◽  
Vol 53 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Aldona Rennert ◽  
Józef Kowalczyk ◽  
Halina Pajkowska
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Pathogenic Bacteria ◽  
Direct Action ◽  
Plant Cells ◽  
Induction Phase ◽  
Bacterial Strains ◽  
Tumour Formation ◽  
Kalanchoë Daigremontiana ◽  
Anti Tumour Effect

With the use of bacterial strains, both sensitive and resistant to hydroxyurea the action of this inhibitor on tumour formation on the leaves of <em>Kalanchoe daigremontiana</em> infected with <em>Agrobacterium tumefaciens</em> was tested for five days after inoculation. The results are in agreement with the opinion that the anti-tumour effect of hydroxyurea applied in the induction phase (between 18 and 60 h after inoculation) is the result of its direct action on plant cells, whereas inhibition of tumour formation by the inhibitor in the inoculation period depends on its action on the pathogenic bacteria.

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