Interactions between genotypes and culture media components for improved in vitro response of rye (Secale cereale L.) inbred lines

2001 ◽  
Vol 20 (7) ◽  
pp. 575-582 ◽  
Author(s):  
J. Popelka ◽  
F. Altpeter
Keyword(s):  
Secale Cereale ◽  
Culture Media ◽  
Inbred Lines ◽  
Secale Cereale L ◽  
In Vitro Response

scholarly journals The identification of QTLs associated with the in vitro response of rye (Secale cereale L.)

2007 ◽  
Vol 12 (4) ◽  
Author(s):  
Hanna Bolibok ◽  
Anna Gruszczyńska ◽  
Aneta Hromada-Judycka ◽  
Monika Rakoczy-Trojanowska
Keyword(s):  
Secale Cereale ◽  
Average Distance ◽  
Recombinant Inbred Lines ◽  
Immature Embryos ◽  
Winter Rye ◽  
Somatic Tissues ◽  
Normal Frequency ◽  
Secale Cereale L ◽  
In Vitro Response

AbstractThis study was conducted in order to identify quantitative trait loci (QTLs) for the in vitro culture response of winter rye (Secale cereale L.) immature embryos and immature inflorescences. A genetic linkage map comprising 67 SSRs, 9 ISSRs, 13 SAMPLs, 7 RAPDs, 2 SCARs and one EST marker was created based on the analyses of 102 recombinant inbred lines from the cross between lines L318 (which has a good response in tissue cultures) and L9 (which is unable to regenerate plants from somatic tissues and anthers). The map spans 979.2 cM, and the average distance between markers is 9.9 cM. Two characteristics were evaluated: callus induction (CI) and somatic embryogenesis ability (SE). They were expressed as the percentage of immature embryos/inflorescences producing callus (designated ECI/ICI) and the percentage of explants producing somatic embryos (ESE/ISE). All the analysed traits showed continuous variation in the mapping population but a non-normal frequency distribution. We identified nine putative QTLs controlling the tissue culture response of rye, explaining up to 41.6% of the total phenotypic variation: two QTLs for ECI — eci-1, eci-2; 4 for ESE — ece-1, ese-2, ese-3, ese-4; 2 for ICI — ici-1, ici2; and 1 for ISE — ise-1. They were detected on chromosomes 1R, 4R, 5R, 6R and 7R.

THE GENETICS OF FERTILITY RESTORATION IN CYTOPLASMIC MALE-STERILE RYE

1979 ◽  
Vol 21 (3) ◽  
pp. 417-422 ◽  
Author(s):  
G. J. Scoles ◽  
L. E. Evans
Keyword(s):  
Secale Cereale ◽  
Fertility Restoration ◽  
Inbred Lines ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Cytoplasmic Male Sterile ◽  
Restorer Genes ◽  
Secale Cereale L ◽  
Partial Fertility

Three inbred lines of rye (Secale cereale L.) known to be capable of restoring fertility to a cytoplasmic male-sterile line were crossed with the sterile line. The proportions of male fertile, partially male fertile and male sterile plants in F2 and backcross progenies indicated that three dominant restorer genes were present in each line. These were designated Rf1, Rf2 and Rf3, their relative expressivity was Rf1>Rf2>Rf3. Expressivity was dependent upon environment. Partial fertility occurred when certain genotypes carried two of the three alleles as dominant, but was dependent upon genotype and environment.

Dynamics of the Tissue-Specific Metabolism of Luteolin Glucuronides in the Mesophyll of Rye Primary Leaves (Secale cereale)

1988 ◽  
Vol 43 (3-4) ◽  
pp. 187-193 ◽  
Author(s):  
Margot Schulz ◽  
Gottfried Weissenböck
Keyword(s):  
Cinnamic Acid ◽  
Secale Cereale ◽  
Leaf Development ◽  
Glucuronic Acid ◽  
Developmental Stages ◽  
Leaf Tissue ◽  
Feeding Experiments ◽  
Secale Cereale L ◽  
Hydrolysis Of

Developing primary leaves of Secale cereale L. exhibit a dynamic metabolism of the major flavonoid luteolin 7-O-[β-ᴅ-glucuronosyl(1→2)β-ᴅ-glucuronide]-4′-O-β-ᴅ-glucuronide (R1). Final steps of R, biosynthesis are sequential glucuronidations of luteolin, which are catalyzed by three specific UDP-glucuronate: flavone glucuronosyltransferases. These enzymes reach highest activities at the fourth and fifth day of leaf development, coinciding with maximal R, accumulation. The activities decrease with advancing age of the leaves. In contrast, a R1-specific β-glucuronidase, responsible for the hydrolysis of glucuronic acid in position 4′, shows increasing activity up to the 5th or 6th day; but this activity, leading to luteolin 7-O-diglucuronide (R2), is not reduced in later developmental stages. In this phase of leaf development, the amount of R, drastically drops, whereas R2 accumulates only slightly. From in vitro results and from feeding experiments using [14C]cinnamic acid, a precursor of R, biosynthesis, we conclude that the anabolic sequential glucuronidation takes place in young and expanding leaf tissue, whereas deglucuronidation occurs in nearly mature and mature tissue. The three glucuronosyltransferases as well as the β-glucuronidase, and the flavonoids R1 and R2 are localized in the mesophyll.

B-chromosomes in inbred lines of rye (Secale cereale L.)

Genetica ◽  
1994 ◽  
Vol 92 (3) ◽  
pp. 149-154 ◽  
Author(s):  
M. M. Jim�nez ◽  
E. Romera ◽  
M. J. Puertas ◽  
R. N. Jones
Keyword(s):  
Secale Cereale ◽  
Inbred Lines ◽  
B Chromosomes ◽  
Secale Cereale L

The effect of rye genotype on wheat–rye crossability and on the development of F1 seed

1983 ◽  
Vol 25 (6) ◽  
pp. 668-670 ◽  
Author(s):  
Graham J. Scoles
Keyword(s):  
Triticum Aestivum ◽  
Secale Cereale ◽  
Seed Set ◽  
Triticum Aestivum L ◽  
Inbred Lines ◽  
Hybrid Seed ◽  
Secale Cereale L

Five inbred lines of rye (Secale cereale L.) and an open-pollinated rye cultivar were used to pollinate wheat (Triticum aestivum L.) cultivars of differeing crossability. No hybrid seed was produced with the cultivar of low crossability, but with the highly crossable cultivar an average seed set of 65% was obtained. Significant differences in terms of seed set and weight of F1 seed were detected among the inbreds.

scholarly journals Influence of Additional Nutrients and Gelling Agents on in Vitro Response of Selected Indica Rice Varieties

2019 ◽  
Vol 11 (4) ◽  
pp. 26
Author(s):  
Sai Krishna Repalli ◽  
Chaitanya Kumar Geda ◽  
N. S. N. Pradhan ◽  
G. J. N. Rao
Keyword(s):  
Culture Media ◽  
Indica Rice ◽  
Casein Hydrolysate ◽  
Gelling Agent ◽  
Regeneration Potential ◽  
Rice Varieties ◽  
Gelling Agents ◽  
Profound Influence ◽  
In Vitro Response

Indica rice varieties are recalcitrant to culture and hence the culture media should be supplemented with additional nutrients to provide energy and osmotic potential for best in vitro response. Combinations of plant growth regulators have profound influence on callus induction and regeneration potential of the selected genotypes. In addition, concentration and choice of gelling agents also have their effect on regeneration of indica rice varieties. Impact of L-Proline, and Casein Hydrolysate on tissue culture response of selected indica rice varieties is discussed and the best choice of gelling agent and their in vitro response is elucidated.

scholarly journals Genetical and anatomical analysis of brittlenses of stems in rye (Secale cereale L.)

2014 ◽  
Vol 50 (4) ◽  
pp. 567-574
Author(s):  
Helena Kubicka ◽  
Bogusław Kubicki
Keyword(s):  
Secale Cereale ◽  
Recessive Gene ◽  
Inbred Lines ◽  
Genetical Analysis ◽  
Anatomical Analysis ◽  
Whole Plant ◽  
Secale Cereale L

Brittlenes of thie stem was found to be one of the more frequently segregating traits in inbred rye lines. In dependenece on the outset cultivated variety this trait appears in the S<sub>2</sub> generation in 4.0-0.6 per cent of the inbred lines. Genetical analysis demonstrated that the trait of brttlenes is determined by one recessive gene denoted by the symbol b<sub>s</sub> (brittlee stem). The bs gene exerts a strong pleliotropic effect on the whole plant, beside brittleness of the stem it causes fragility of the roots, heads and leaves and depresses the general viability of the plants. Anatomilcal observations of thie stem and root sholwed that the b<sub>s</sub> gene causes disturbances in the norirmal lignifcation of the sclerenichyma cells both in shoots and roots, so that these cells are th1i-walled. The tickness of the sclerenchyma layers and the number and size of the vasclcuar in the brittle forms are significantly smaller than in the normal ones. It was found that these changes appear in the bittle forms at the phase of heading of the plants.

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