scholarly journals Alkaloid production by a Cinchona officinalis 'Ledgeriana' hairy root culture containing constitutive expression constructs of tryptophan decarboxylase and strictosidine synthase cDNAs from Catharanthus roseus

1999 ◽  
Vol 19 (2) ◽  
pp. 191-196 ◽  
Author(s):  
A. Geerlings ◽  
D. Hallard ◽  
A. Martinez Caballero ◽  
I. Lopes Cardoso ◽  
R. van der Heijden ◽  
...  
Keyword(s):  
Hairy Root ◽  
Catharanthus Roseus ◽  
Constitutive Expression ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Tryptophan Decarboxylase ◽  
Alkaloid Production ◽  
Strictosidine Synthase

scholarly journals Effects of over-expression of strictosidine synthase and tryptophan decarboxylase on alkaloid production by cell cultures of Catharanthus roseus

Planta ◽  
1998 ◽  
Vol 205 (3) ◽  
pp. 414-419 ◽  
Author(s):  
Camilo Canel ◽  
M. Inês Lopes-Cardoso ◽  
Serap Whitmer ◽  
Leslie van der Fits ◽  
Giancarlo Pasquali ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Catharanthus Roseus ◽  
Tryptophan Decarboxylase ◽  
Alkaloid Production ◽  
Strictosidine Synthase ◽  
Over Expression

Biosynthetic system of camptothecin: An anticancer plant product

2010 ◽  
Vol 82 (1) ◽  
pp. 213-218 ◽  
Author(s):  
Mami Yamazaki ◽  
Takashi Asano ◽  
Yasuyo Yamazaki ◽  
Supaart Sirikantaramas ◽  
Hiroshi Sudo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Hairy Root ◽  
Plant Cells ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Pathway Engineering ◽  
Strictosidine Synthase ◽  
Anticancer Compounds ◽  
Plant Product

Camptothecin is one of the clinically used anticancer compounds derived from plants. We have established a hairy root culture Ophiorrhiza pumila, which efficiently produces camptothecin. The strictosidine synthase cDNA was obtained from O. pumila, and its properties were characterized using recombinant protein expressed in Escherichia coli. The mechanisms of camptothecin transport and self-resistance of producing plant cells have also been investigated. These studies offer a basis for pathway engineering of camptothecin in the future.

scholarly journals Pengaruh elisitasi terhadap pertumbuhan dan produksi alkaloida kinolin dari akar rambut tanaman kina (Cinchona succirubra Pavon ex Klotzsch) Effect of elicitation on growth and alkaloid quinoline production in hairy root of cinchona plant (Cinchona succirubra Pavon ex Klotzsch)

2016 ◽  
Vol 74 (1) ◽  
Author(s):  
Nurita TORUAN-MATHIUS ◽  
. NURHAIMI-HARIS ◽  
Joko SANTOSO ◽  
. ADE-HERI
Keyword(s):  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Alkaloid Content ◽  
Alkaloid Production ◽  
Fresh Weight ◽  
Quinoline Alkaloid

Summary Manipulation to increase alkaloid producti-vity in hairy root  can be done by elicitation with the addition of certain  enzymes. The objective of  this research is to find out the effect of elicitation by cellulase and pectyoliase enzymes on the productivity of Cinchona succirubra  hairy root culture. Hairy root was  cultured on ½ MS macro nutrient with the addition of 40 g/L sucrose and  7 g/L agar. Elicitation was done by the addition of cellulase and pectiolyase at the concentrations of 0; 0.05; 0.10; 0.50; 1.00; 3.00; 5.00; 10.00; 15.00; 20.00 and 25.00 mg/L, respectively.The effect of elicitation was  analyzed by fresh weight of hairy root, and alkaloid content in four-month-old culture.The result showed that the best growth was obtained by the addition of 15 mg/L cellulase, with fresh weight as much as  920 mg. The addition of 10 mg/L pectyoliase increased  hairy root fresh weight as much as  880 mg. The highest quinoline alkaloid production was obtained by the addition of 25 mg/L celluase (Quinine 580, quinidine 492, cinchonine 234, dihydroxyn-chonine 195 and cinchonidine 165 µg/g fresh weight) and 1 mg/L pectyoliase (Quinine 2363, quinidine 238, cinchonine 104, dihydroxyn-chonidine 138 and cinchonidine 1558 µg/g fresh weight).Ringkasan Manipulasi untuk meningkatkan produk-tivitas  akar rambut  dapat dilakukan di antaranya dengan elisitasi melalui penambahan enzim tertentu ke dalam medium. Tujuan penelitian ini adalah untuk menetapkan pengaruh elisitasi dengan penambahan selulase dan pektioliase terhadap produktivitas akar rambut tanaman Cinchona succirubra. Akar rambut tanaman Cinchona succirubradikulturkan dalam medium MS ½ hara makro dengan penambahan sukrose 40 g/L dan agar 7 g/L. Elisitasi dilakukan dengan penambahan selulase dan pektioliase  masing-masing pada konsentrasi 0; 0,05; 0,10; 0,50; 1,00; 3,00; 5,00; 10,00; 15,00; 20,00 dan 25,00 mg/L. Peubah yang diukur adalah bobot basah akar rambut dan kandungan alkaloida kinolin pada kultur berumur empat bulan. Hasil yang diperoleh menunjukkan bahwa pertumbuh-an terbaik terjadi pada  penambahan konsentrasi selulase 15 mg/L  dengan bobot basah 920 mg dan pektioliase  10 mg/L dengan bobot basah   880 mg. Produksi alkaloida kinolin tertinggi  diperoleh   pada   konsentrasi   selulase  25 mg/L (kinin 580, kinidin 492, sinkonin 234, dihidrok-sinkonin 195 dan sinkonidin 165 µg/g bobot basah) dan pektioliase 1 mg/L (kinin 2363, kinidin 238, sinkonin 104, dihidroksinkonin 138 dan sinkonidin 1558 µg/g bobot basah).   

scholarly journals Pengaruh elisitasi terhadap pertumbuhan dan produksi alkaloida kinolin dari akar rambut tanaman kina (Cinchona succirubra Pavon ex Klotzsch) Effect of elicitation on growth and alkaloid quinoline production in hairy root of cinchona plant (Cinchona succirubra Pavon ex Klotzsch)

2016 ◽  
Vol 74 (1) ◽  
Author(s):  
Nurita TORUAN-MATHIUS ◽  
. NURHAIMI-HARIS ◽  
Joko SANTOSO ◽  
. ADE-HERI
Keyword(s):  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Alkaloid Content ◽  
Alkaloid Production ◽  
Fresh Weight ◽  
Quinoline Alkaloid

Summary Manipulation to increase alkaloid producti-vity in hairy root  can be done by elicitation with the addition of certain  enzymes. The objective of  this research is to find out the effect of elicitation by cellulase and pectyoliase enzymes on the productivity of Cinchona succirubra  hairy root culture. Hairy root was  cultured on ½ MS macro nutrient with the addition of 40 g/L sucrose and  7 g/L agar. Elicitation was done by the addition of cellulase and pectiolyase at the concentrations of 0; 0.05; 0.10; 0.50; 1.00; 3.00; 5.00; 10.00; 15.00; 20.00 and 25.00 mg/L, respectively.The effect of elicitation was  analyzed by fresh weight of hairy root, and alkaloid content in four-month-old culture.The result showed that the best growth was obtained by the addition of 15 mg/L cellulase, with fresh weight as much as  920 mg. The addition of 10 mg/L pectyoliase increased  hairy root fresh weight as much as  880 mg. The highest quinoline alkaloid production was obtained by the addition of 25 mg/L celluase (Quinine 580, quinidine 492, cinchonine 234, dihydroxyn-chonine 195 and cinchonidine 165 µg/g fresh weight) and 1 mg/L pectyoliase (Quinine 2363, quinidine 238, cinchonine 104, dihydroxyn-chonidine 138 and cinchonidine 1558 µg/g fresh weight).Ringkasan Manipulasi untuk meningkatkan produk-tivitas  akar rambut  dapat dilakukan di antaranya dengan elisitasi melalui penambahan enzim tertentu ke dalam medium. Tujuan penelitian ini adalah untuk menetapkan pengaruh elisitasi dengan penambahan selulase dan pektioliase terhadap produktivitas akar rambut tanaman Cinchona succirubra. Akar rambut tanaman Cinchona succirubradikulturkan dalam medium MS ½ hara makro dengan penambahan sukrose 40 g/L dan agar 7 g/L. Elisitasi dilakukan dengan penambahan selulase dan pektioliase  masing-masing pada konsentrasi 0; 0,05; 0,10; 0,50; 1,00; 3,00; 5,00; 10,00; 15,00; 20,00 dan 25,00 mg/L. Peubah yang diukur adalah bobot basah akar rambut dan kandungan alkaloida kinolin pada kultur berumur empat bulan. Hasil yang diperoleh menunjukkan bahwa pertumbuh-an terbaik terjadi pada  penambahan konsentrasi selulase 15 mg/L  dengan bobot basah 920 mg dan pektioliase  10 mg/L dengan bobot basah   880 mg. Produksi alkaloida kinolin tertinggi  diperoleh   pada   konsentrasi   selulase  25 mg/L (kinin 580, kinidin 492, sinkonin 234, dihidrok-sinkonin 195 dan sinkonidin 165 µg/g bobot basah) dan pektioliase 1 mg/L (kinin 2363, kinidin 238, sinkonin 104, dihidroksinkonin 138 dan sinkonidin 1558 µg/g bobot basah).   

scholarly journals Advances and Perspectives in Tissue Culture and Genetic Engineering of Cannabis

2021 ◽  
Vol 22 (11) ◽  
pp. 5671
Author(s):  
Mohsen Hesami ◽  
Austin Baiton ◽  
Milad Alizadeh ◽  
Marco Pepe ◽  
Davoud Torkamaneh ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Genetic Engineering ◽  
Suspension Culture ◽  
Secondary Metabolite ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Gene Transformation ◽  
Secondary Metabolite Production ◽  
Metabolite Production

For a long time, Cannabis sativa has been used for therapeutic and industrial purposes. Due to its increasing demand in medicine, recreation, and industry, there is a dire need to apply new biotechnological tools to introduce new genotypes with desirable traits and enhanced secondary metabolite production. Micropropagation, conservation, cell suspension culture, hairy root culture, polyploidy manipulation, and Agrobacterium-mediated gene transformation have been studied and used in cannabis. However, some obstacles such as the low rate of transgenic plant regeneration and low efficiency of secondary metabolite production in hairy root culture and cell suspension culture have restricted the application of these approaches in cannabis. In the current review, in vitro culture and genetic engineering methods in cannabis along with other promising techniques such as morphogenic genes, new computational approaches, clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR/Cas9-equipped Agrobacterium-mediated genome editing, and hairy root culture, that can help improve gene transformation and plant regeneration, as well as enhance secondary metabolite production, have been highlighted and discussed.

A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum

Biologia ◽  
2014 ◽  
Vol 69 (7) ◽  
Author(s):  
Elnaz Nourozi ◽  
Bahman Hosseini ◽  
Abbas Hassani
Keyword(s):  
Salicylic Acid ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
High Performance ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Biochemical Properties ◽  
Separate Experiment ◽  
Transformed Roots

AbstractHairy root culture system is a valuable tool to study the characteristics of gene expression, gene function, root biology, biochemical properties and biosynthesis pathways of secondary metabolites. In the present study, hairy roots were established in Anise hyssop (Agastache foeniculum) via Agrobacterium rhizogenes. Three strains of Agrobacterium rhizogenes (A4, A7 and 9435), were used for induction of hairy roots in four various explants (hypocotyl, cotyledon, one-month-old leaf and five-month-old leaf) of Anise hyssop. The highest frequency of transformation was achieved using A4 strain in one-month-old leaves (51.1%). The transgenic states of hairy root lines were confirmed by PCR (Polymerase chain reaction) method. High performance liquid chromatography analysis revealed that the production of rosmarinic acid (RA) in transformed roots of A. foeniculum was almost 4-fold higher than that of the non-transformed roots. In a separate experiment, hairy roots obtained from one-month-old leaves inoculated with A4 strain, were grown in liquid medium and the effects of different concentrations of salicylic acid (0.0, 0.01, 0.1 and 1 mM) and chitosan (0, 50, 100 and 150 mg L−1) (as elicitor) and sucrose (20, 30, 40 and 50 g L−1) on the growth of hairy roots were evaluated. The results showed that, 30 g L−1 sucrose and 100 mg L−1 chitosan increased the biomass of hairy root cultures and application of salicylic acid reduced the growth of hairy roots compared with control roots.

Purification and stabilization of ricin B from tobacco hairy root culture medium by aqueous two-phase extraction

2005 ◽  
Vol 117 (1) ◽  
pp. 39-48 ◽  
Author(s):  
Chenming Zhang ◽  
Fabricio Medina-Bolivar ◽  
Scott Buswell ◽  
Carole L. Cramer
Keyword(s):  
Hairy Root ◽  
Culture Medium ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Phase Extraction ◽  
Two Phase ◽  
Aqueous Two Phase Extraction
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