Enhancement of plant regeneration from embryogenic callus of commercial barley cultivars

1998 ◽  
Vol 17 (12) ◽  
pp. 941-945 ◽  
Author(s):  
P. Bregitzer ◽  
L. S. Dahleen ◽  
R. D. Campbell
Keyword(s):  
Plant Regeneration ◽  
Embryogenic Callus ◽  
Barley Cultivars

scholarly journals High frequency plant regeneration from embryogenic callus of a popular indica rice (Oryza sativa L.)

2009 ◽  
Vol 15 (4) ◽  
pp. 371-375 ◽  
Author(s):  
Alagarsamy Karthikeyan ◽  
Shunmugiah Thevar Karutha Pandian ◽  
Manikandan Ramesh
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
High Frequency ◽  
Embryogenic Callus ◽  
Oryza Sativa L ◽  
Indica Rice

scholarly journals Somatic embryogenesis and plant regeneration from barley cultivars grown in Spain

1998 ◽  
Vol 17 (11) ◽  
pp. 902-906 ◽  
Author(s):  
A. M. Castillo ◽  
B. Egaña ◽  
J. M. Sanz ◽  
L. Cistué
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Barley Cultivars

A comparative study of initiation and development of embryogenic callus from haploid embryos of several barley cultivars. II. Cytophotometry of embryos and callus

1986 ◽  
Vol 64 (9) ◽  
pp. 2107-2112 ◽  
Author(s):  
L. S. Kott ◽  
S. Flack ◽  
K. J. Kasha
Keyword(s):  
Hordeum Vulgare ◽  
Comparative Study ◽  
Ploidy Level ◽  
Embryogenic Callus ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Haploid Embryos ◽  
Diploid Level

Cells of haploid barley embryos (Hordeum vulgare L. 'Bruce', 'Perth', and 'Klages') and callus cells originating from embryos were cytophotometrically examined to determine the ploidy level. Specific embryo tissues regularly exhibited predictable ploidies and smaller embryos had a higher percentage of haploid cells than larger embryos of the same age. The predominantly haploid cells of the scutellar epidermis of the embryo initiated callus which generally, at least initially, was haploid. Monitoring of the ploidy evolution of these haploid calli showed that over a 6-month period each line exhibited its own unique rate of polyploidization, although lines of the same cultivar showed similar trends. Accumulation of cells at the diploid level was often a characteristic of these cultures.

A comparative study of initiation and development of embryogenic callus from haploid embryos of several barley cultivars. I. Developmental state of embryo explants and callus potential

1987 ◽  
Vol 65 (1) ◽  
pp. 53-56 ◽  
Author(s):  
L. S. Kott ◽  
E. Kott ◽  
M. Howarth ◽  
K. J. Kasha
Keyword(s):  
Comparative Study ◽  
Rapid Growth ◽  
Embryogenic Callus ◽  
Growth Period ◽  
Developmental State ◽  
Root Initiation ◽  
Barley Cultivars ◽  
Haploid Embryos ◽  
Haploid Callus ◽  
Secondary Shoot

Six cultivars of barley (‘Akka’, ‘Bruce’, ‘Klages’, ‘Leger’, ‘Perth’, and ‘York’) were compared for their ability to produce haploid embryos competent to produce embryogenic haploid callus. The capacity to generate such callus from scutellar epidermal cells is different among these cultivars and appears to be correlated with the developmental state–age of the embryo and associated with a rapid growth period. Histological examination of 12-, 14-, and 16-day-old embryos confirmed the meristematic nature of the callus-initiating cells and produced results which correspond well with results obtained from cultures of other similar embryos. Variability among cultivars in embryo development, root initiation from callus, and primary and secondary shoot regeneration was also identified.

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