Activated charcoal affects morphogenesis and enhances sporophyte regeneration during leaf cell suspension culture of Platycerium bifurcatum

1997 ◽  
Vol 17 (2) ◽  
pp. 77-83 ◽  
Author(s):  
W.-L. Teng
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Activated Charcoal ◽  
Leaf Cell ◽  
Platycerium Bifurcatum

scholarly journals Optimization of Coconut Micropropagation via Somatic Embryogenesis

Proceedings ◽  
2020 ◽  
Vol 36 (1) ◽  
pp. 55
Author(s):  
Mu
Keyword(s):  
Somatic Embryogenesis ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Activated Charcoal ◽  
Clonal Propagation ◽  
Medium Composition ◽  
Induction Medium ◽  
Multiplication Rate ◽  
Auxin Concentration

Coconut (Cocos nucifera L.) is one of the most important tropical palm crops worldwide, but the gap between decreasing productivity and expanding demand is growing and is now becoming a major problem. The global coconut industry is threatened not only by palm senility but by pests/pathogen attack which is becoming exacerbated by climate change. However, conventional breeding methods cannot provide sufficient new planting materials to meet the replacement of senile and damaged palms. Hence, coconut clonal propagation via somatic embryogenesis (SE) is being developed to help meet the demand of the growing market. However, with refined medium composition, improved technical skills, optimized environmental conditions, proper acclimatization steps employed and the application of a cell suspension culture step, a much higher multiplication rate can be predicted. Several factors that affect the rate of clonal propagation are being studied: 1) determination of the effect of a non-activated charcoal (AC) medium and 2) the development of culture induction medium composition with a reduced auxin concentration. Results of the study so far show that a non-activated charcoal medium with more frequent subculture (monthly) and a lower auxin concentration is an efficient alternative approach to the traditional mainstream AC-containing medium for the initiation of embryogenic callus. With a better understanding of the callus induction medium composition, and with a liquid cell suspension culture step medium to be applied in the future, should elevate the multiplication rate of clonal plantlet production.

scholarly journals In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

2009 ◽  
Vol 23 (1) ◽  
pp. 274-281 ◽  
Author(s):  
Tiago Santana Balbuena ◽  
Claudete Santa-Catarina ◽  
Vanildo Silveira ◽  
Massuo Jorge Kato ◽  
Eny Iochevet Segal Floh
Keyword(s):  
Plant Growth ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Activated Charcoal ◽  
Leaf Explants ◽  
Biologically Active ◽  
In Vitro Morphogenesis ◽  
Fresh Matter

Piper solmsianum is a shrub from Southeast Brazil in which many biologically active compounds were identified. The aim of this work was to establish a cell suspension culture system for this species. With this in mind, petiole and leaf explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D and BA). Root and indirect shoot adventitious formation, detected by histological analysis, was observed. Besides the different combinations of plant growth regulators, light regime and the supplement of activated charcoal (1.5 mg.l-1) were tested for callus induction and growth. Cultures maintained in light, on a 0.2 mg.l-1 2,4-D and 2 mg.l-1 BA supplemented medium, and in the absence of activated charcoal, showed the highest calli fresh matter increment. From a callus culture, cell suspension cultures were established and their growth and metabolite accumulation studied. The achieved results may be useful for further characterization of the activated secondary metabolites pathways in in vitro systems of P. solmsianum.

scholarly journals Citrus Cell Suspension Culture Establishment, Maintenance, Efficient Transformation and Regeneration to Complete Transgenic Plant

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 664
Author(s):  
M. Moniruzzaman ◽  
Yun Zhong ◽  
Zhifeng Huang ◽  
Huaxue Yan ◽  
Lv Yuanda ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Culture System ◽  
Transformation Rate ◽  
Suspension Cell Culture ◽  
Citrus Reticulata ◽  
Agrobacterium Mediated Transformation ◽  
Solid Media

Agrobacterium-mediated transformation of epicotyl segment has been used in Citrus transgenic studies. The approach suffers, however, from limitations such as occasionally seed unavailability, the low transformation efficiency of juvenile tissues and the high frequency of chimeric plants. Therefore, a suspension cell culture system was established and used to generate transgenic plants in this study to overcome the shortcomings. The embryonic calli were successfully developed from undeveloped ovules of the three cultivars used in this study, “Sweet orange”-Egyptian cultivar (Citrus sinensis), “Shatangju” (Citrus reticulata) and “W. Murcott” (Citrus reticulata), on three different solid media. Effects of media, genotypes and ages of ovules on the induction of embryonic calli were also investigated. The result showed that the ovules’ age interferes with the callus production more significantly than media and genotypes. The 8 to 10 week-old ovules were found to be the best materials. A cell suspension culture system was established in an H+H liquid medium. Transgenic plants were obtained from Agrobacterium-mediated transformation of cell suspension as long as eight weeks subculture intervals. A high transformation rate (~35%) was achieved by using our systems, confirming BASTA selection and later on by PCR confirmation. The results demonstrated that transformation of cell suspension should be more useful for the generation of non-chimeric transgenic Citrus plants. It was also shown that our cell suspension culture procedure was efficient in maintaining the vigor and regeneration potential of the cells.

scholarly journals Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew

2014 ◽  
Vol 38 ◽  
pp. 76-82 ◽  
Author(s):  
Zohreh JALALPOUR ◽  
Leila SHABANI ◽  
Ladan AFGHANI ◽  
Majid SHARIFI-TEHRANI ◽  
Sayed-Asadollah AMINI
Keyword(s):  
Suspension Culture ◽  
Methyl Jasmonate ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Phenolic Metabolism
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