Rapid clonal propagation of three mulberries, Morus cathayana Hemsl., M. lhou Koiz. and M. serrata Roxb., through in vitro culture of apical shoot buds and nodal explants from mature trees

1997 ◽  
Vol 16 (7) ◽  
pp. 503-508
Author(s):  
S. K. Pattnaik ◽  
P. K. Chand
Keyword(s):  
In Vitro Culture ◽  
Clonal Propagation ◽  
Nodal Explants ◽  
Mature Trees ◽  
Shoot Buds ◽  
Apical Shoot

Rapid clonal propagation of rhubarb by in vitro culture of shoot-tips

1979 ◽  
Vol 11 (3) ◽  
pp. 241-246 ◽  
Author(s):  
Joseph Roggemans ◽  
Marie-Christine Claes
Keyword(s):  
In Vitro Culture ◽  
Clonal Propagation ◽  
Shoot Tips

scholarly journals Inhibition of browning problem during the callogenesis of Spartium junceum L.

2021 ◽  
Vol 27 (1) ◽  
pp. 68-77
Author(s):  
Mina Taghizadeh ◽  
Mahboubeh Ganji Dastjerdi
Keyword(s):  
In Vitro Culture ◽  
Callus Formation ◽  
Nodal Explants ◽  
Antioxidant Compounds ◽  
Induction Phase ◽  
Natural Habitats ◽  
Running Water ◽  
Changing Light ◽  
Spartium Junceum

Abstract During different phases of in vitro culture, plant tissues may be exposed to some stresses that never encounter in their natural habitats. The most significant stresses which interfere with in vitro culture are pathogenic contamination and browning disorder. Since browning sign is occurred during all phases of in vitro culture of Spartium junceum L., the present study was done preventing explants from browning during disinfection and callogenesis phases using exposure time of sterilants (ethanol 0, 30, 60 s and home bleach 0, 10, 15 min), antioxidant compounds (PVP 0.5%, Activated charcoal 0.1%, Curcumin 0.1%), Running water (30 and 60 min) plant growth regulators (2,4-D 0, 0.5, 1 and 2 mg L-1 and BA 0, 0.1 and 0.2 mg L-1), and by changing light/dark conditions was designed. The results showed that ethanol 70% (30 s) in combination with home bleach 20% (10 min) had the best effect in control contaminations and browning sign in nodal explants of S. junceum. The application of PVP 0.5% in medium was the best treatment to control of browning nodal explants in callus induction phase. The highest callus formation and the lowest explant browning were obtained on the medium supplemented with 0.5 mg L-1 2,4-D under the darkness condition. According to the results of this study, how disinfection methods, culture medium compositions and light conditions were effective on the browning and callogenesis of Spartium junceum L.

scholarly journals Regeneration of Pinus halepensis (Mill.) through Organogenesis from Apical Shoot Buds

Forests ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 363
Author(s):  
Cátia Pereira ◽  
Itziar A. Montalbán ◽  
Ana Pedrosa ◽  
Jéssica Tavares ◽  
Alexey Pestryakov ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Pinus Halepensis ◽  
Culture Media ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Embryogenic Calli ◽  
Shoot Buds ◽  
Apical Shoot ◽  
Adult Trees

Organogenesis and somatic embryogenesis have been widely applied as the two main regeneration pathways in plant tissue cultures. However, recalcitrance is still the main restriction in the clonal propagation of many woody species, especially in conifers. They undergo a “phase change” that leads to significant loss of vegetative propagation capacity, reducing the aptitude of tissues and organs to be regenerated in vitro beyond this point. In line with this, the in vitro regeneration of mature conifer trees has been a long-cherished goal in many laboratories worldwide. Based on previous works in Pinus species regeneration from adult trees, we now present data about the culture of apical shoot buds in an attempt to induce organogenesis and somatic embryogenesis to clone mature trees of Aleppo pine (Pinus halepensis). Reinvigorated axillary shoots were submitted to conditions usually applied to induce somatic embryogenesis through the manipulation of culture media, including the use of auxins such as 2,4-Dichlorophenoxyacetic acid and 1-Naphthaleneacetic acid, cytokinins (6-benzyladenine and kinetin), and phytosulfokine (50, 100, and 200 nM). Although somatic embryos could not be obtained, an embryogenic-like tissue was produced, followed by the emergence of actively proliferating non-embryogenic calli. Variations in the consistence, texture, and color of non-embryogenic calli were observed; especially those arising in the media containing phytosulfokine. Reinvigorated shoots, induced by 22 or 44 µM 6-benzyladenine, were obtained through organogenesis and acclimatized, and phenotypically normal plants were obtained.

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