Micropropagation of Panax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets

1997 ◽  
Vol 16 (7) ◽  
pp. 450-453 ◽  
Author(s):  
Y. Shoyama ◽  
Xuan Xuan Zhu ◽  
R. Nakai ◽  
S. Shiraishi ◽  
H. Kohda
Keyword(s):  
Somatic Embryogenesis ◽  
Rapd Analysis ◽  
Panax Notoginseng ◽  
Regenerated Plantlets

Micropropagation ofPanax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets

1997 ◽  
Vol 16 (7) ◽  
pp. 450-453
Author(s):  
Y. Shoyama ◽  
X. X. Zhu ◽  
R. Nakai ◽  
S. Shiraishi ◽  
H. Kohda
Keyword(s):  
Somatic Embryogenesis ◽  
Rapd Analysis ◽  
Regenerated Plantlets

scholarly journals MicroRNA Zma-miR528 Versatile Regulation on Target mRNAs during Maize Somatic Embryogenesis

2021 ◽  
Vol 22 (10) ◽  
pp. 5310
Author(s):  
Eduardo Luján-Soto ◽  
Vasti T. Juárez-González ◽  
José L. Reyes ◽  
Tzvetanka D. Dinkova
Keyword(s):  
Somatic Embryogenesis ◽  
Immature Embryo ◽  
Inverse Correlation ◽  
Total Rna ◽  
Target Mrnas ◽  
Sequence Complementarity ◽  
Low Levels ◽  
Embryogenic Callus Induction ◽  
Regenerated Plantlets ◽  
Polysome Fraction

MicroRNAs (miRNAs) are small non-coding RNAs that regulate the accumulation and translation of their target mRNAs through sequence complementarity. miRNAs have emerged as crucial regulators during maize somatic embryogenesis (SE) and plant regeneration. A monocot-specific miRNA, mainly accumulated during maize SE, is zma-miR528. While several targets have been described for this miRNA, the regulation has not been experimentally confirmed for the SE process. Here, we explored the accumulation of zma-miR528 and several predicted targets during embryogenic callus induction, proliferation, and plantlet regeneration using the maize cultivar VS-535. We confirmed the cleavage site for all tested zma-miR528 targets; however, PLC1 showed very low levels of processing. The abundance of zma-miR528 slightly decreased in one month-induced callus compared to the immature embryo (IE) explant tissue. However, it displayed a significant increase in four-month sub-cultured callus, coincident with proliferation establishment. In callus-regenerated plantlets, zma-miR528 greatly decreased to levels below those observed in the initial explant. Three of the target transcripts (MATE, bHLH, and SOD1a) showed an inverse correlation with the miRNA abundance in total RNA samples at all stages. Using polysome fractionation, zma-miR528 was detected in the polysome fraction and exhibited an inverse distribution with the PLC1 target, which was not observed at total RNA. Accordingly, we conclude that zma-miR528 regulates multiple target mRNAs during the SE process by promoting their degradation, translation inhibition or both.

CYCLAMEN PERSICUM MILL.: SOMATIC EMBRYOGENESIS AND RAPD ANALYSIS OF EMBRYOGENIC CALLUS

2003 ◽  
pp. 137-144 ◽  
Author(s):  
M. Laura ◽  
L. De Benedetti ◽  
.S Bruna ◽  
G. Burchi ◽  
T. Berio ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Rapd Analysis ◽  
Cyclamen Persicum

scholarly journals In vitro regeneration and somatic embryogenesis in citrus

2015 ◽  
Vol 24 (2) ◽  
pp. 247-262 ◽  
Author(s):  
El Sawy A Mohamed ◽  
Amina Gomaa ◽  
Nancy Danial
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Stability ◽  
Somatic Embryos ◽  
Rapd Analysis ◽  
In Vitro Regeneration ◽  
Rt Pcr ◽  
Mother Plants

Better results were obtained when stigma explants of variegated lemon and citron were used. After ten months, somatic embryos developed into plantlets at a frequency ranged from 13.3 for lime to 66.7% for lemon. Virus presence was tested by ELISA and RT?PCR. The results indicated that the plantlets regenerated through somatic embryogenesis are CTV?free. RAPD analysis was used to asses the genetic stability of plantlets as compared to the mother plants. The results indicated that most plantlets belong to the respective mother plants and the polymorphism percentage was genotype and explant?dependant.Plant Tissue Cult. & Biotech. 24(2): 247-262, 2014 (December

scholarly journals Plant regeneration through somatic embryogenesis from calli derived from leaf bases of Laurus nobilis L. (Lauraceae)

2015 ◽  
Vol 24 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Ahmad H Al Gabbiesh ◽  
M Ghabeish ◽  
I H ◽  
M Kleinwächter ◽  
D Selmar
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Root Formation ◽  
Callus Formation ◽  
Germplasm Conservation ◽  
Laurus Nobilis ◽  
The Media ◽  
Regenerated Plantlets ◽  
Culture Technology

Somatic embryogenesis was induced in embryo culture on half MS medium supplemented with NAA (8 mg/l) as the sole plant growth regulator after incubation of the media in the refrigerator at 4°C for two weeks to promote callus induction and somatic embryogenesis in Laurus nobilis. Both embryogenetic calli and somatic embryos were induced in the above selected medium. Embryo growth and development were stimulated by separation of embryos successfully from embryo clusters and transferred onto fresh half MS. Among the selected explants, only leaf bases were found to respond actively to plant regeneration, especially in inducing callus formation and in sustaining faster callus growth. Root formation of regenerated plantlets tended to decrease with time on regeneration media. Overall, 75% of the plantlets derived from the callus survived in the greenhouse; and they all grew to phenotypically normal plants. This procedure will enable the use of regeneration tissue culture technology for germplasm conservation of L. nobilis, a plant of high medicinal and commercial value.Plant Tissue Cult. & Biotech. 24(2): 213-221, 2014 (December)

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