Genetic tranformation of cotyledon explants of cowpea (Vigna unguiculata L. Walp) using Agrobacterium tumefaciens

1996 ◽  
Vol 15 (12) ◽  
pp. 980-985 ◽  
Author(s):  
B. Muthukumar ◽  
M. Mariamma ◽  
K. Veluthambi ◽  
A. Gnanam
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Vigna Unguiculata ◽  
Cotyledon Explants

scholarly journals Agrobacterium-mediated Transformation of Cotyledon Explants of Bottle Gourd (Lagenaria siceraria S.)

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 821E-822
Author(s):  
Jeung-Sul Han* ◽  
Chang Kil Kim
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Binary Vector ◽  
Bottle Gourd ◽  
Infection Frequency ◽  
Ms Medium ◽  
Lagenaria Siceraria ◽  
Cultivation Medium ◽  
Glufosinate Ammonium ◽  
Cotyledon Explants ◽  
Selection Medium

A procedure for producing transgenic bottle gourd plants by inoculating cotyledon explants with Agrobacterium tumefaciens strain AGL1 carrying a binary vector pCAMBIA3301, which contains glufosinate ammonium-resistant (bar) and the reporter (gus) genes, is describe. Infection was the most effective (highest infection frequency and index) when explants were co-cultivated with Agrobacterium for 6-8 days on co-cultivation medium supplemented with 0.001-0.1 mg/L L-a-(2-aminoethoxyvinyl) glycine (AVG). Transgenic plants were obtained with frequencies of about 0.2% when the explants were cultured on selection medium (MS medium supplemented with 3.0 mg/L BAP, 0.5 mg/L AgNO3, 500 mg/L cefotaxime, 2.0 mg/L DL-phosphinothricin, 0.3% sucrose and 0.8% Plant Agar. A histochemical gus assay, PCR and Southern blot analyses confirmed that transformation had occurred. Genetic analysis of T1 progenies showed that the transgenes were inherited in a Mendelian fashion. To our knowlege, this study represents the first report for Agrobacterium-mediated transformation in bottle gourd, rootstock for watermelon and other cucurbit crops in many countries.

Agrobacterium tumefaciens-mediated high frequency genetic transformation of an Indian cowpea (Vigna unguiculata L. Walp.) cultivar and transmission of transgenes into progeny

Plant Science ◽  
2007 ◽  
Vol 172 (4) ◽  
pp. 692-700 ◽  
Author(s):  
Darshna Chaudhury ◽  
Seema Madanpotra ◽  
Ranjana Jaiwal ◽  
Raman Saini ◽  
P. Ananda Kumar ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Vigna Unguiculata ◽  
High Frequency

scholarly journals An effective method for Agrobacterium tumefaciens-mediated transformation of Jatropha curcas L. using cotyledon explants

Bioengineered ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 1146-1158
Author(s):  
Ying Liu ◽  
Xiaoyan Yang ◽  
Yahuan Zhao ◽  
Yuesheng Yang ◽  
Zhenlan Liu
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Jatropha Curcas ◽  
Jatropha Curcas L ◽  
Cotyledon Explants
Sign in / Sign up

Export Citation Format

Share Document