Induction of direct somatic embryogenesis and plant regeneration in pepper ( Capsicum annuum L.)

1996 ◽  
Vol 15 (7) ◽  
pp. 536-540
Author(s):  
Sangeeta Joshi ◽  
N. Sankhla ◽  
Marla L. Binzel ◽  
Daksha Sankhla
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Capsicum Annuum ◽  
Direct Somatic Embryogenesis ◽  
Capsicum Annuum L

Induction of direct somatic embryogenesis and plant regeneration in pepper (Capsicum annuum L.)

1996 ◽  
Vol 15 (7) ◽  
pp. 536-540 ◽  
Author(s):  
Marla L. Binzel ◽  
N. Sankhla ◽  
Sangeeta Joshi ◽  
Daksha Sankhla
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Capsicum Annuum ◽  
Direct Somatic Embryogenesis ◽  
Capsicum Annuum L

Pepper (capsicum annuum L.) regenerants obtained by direct somatic embryogenesis fail to develop a shoot

2003 ◽  
Vol 39 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Benjamin Steinitz ◽  
Mustafa Küsek ◽  
Yona Tabib ◽  
Ilan Paran ◽  
Aaron Zelcer
Keyword(s):  
Somatic Embryogenesis ◽  
Capsicum Annuum ◽  
Direct Somatic Embryogenesis ◽  
Capsicum Annuum L

Direct somatic embryogenesis and plant regeneration from immature explants of chickpea

2010 ◽  
Vol 54 (1) ◽  
pp. 121-125 ◽  
Author(s):  
S. Ghanti ◽  
K. G. Sujata ◽  
M. Rao ◽  
P. B. Kishor
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Direct Somatic Embryogenesis

Direct somatic embryogenesis and plant regeneration from protoplasts of red clover (Trifolium pratense L.)

Plant Science ◽  
1994 ◽  
Vol 97 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Maxim A. Radionenko ◽  
Nikolay V. Kuchuk ◽  
Olga A. Khvedynich ◽  
Yuri Yu. Gleba
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Direct Somatic Embryogenesis ◽  
Trifolium Pratense L

scholarly journals NAA at a high concentration promotes efficient plant regeneration via direct somatic embryogenesis and SE-mediated transformation system in Ranunculus sceleratus

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ke-dong Xu ◽  
Wei Wang ◽  
De-shui Yu ◽  
Xiao-li Li ◽  
Jia-min Chen ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Marker Gene ◽  
Direct Somatic Embryogenesis ◽  
The Novel ◽  
Transformation System ◽  
High Concentration ◽  
High Frequencies ◽  
Classical Pattern ◽  
Flower Organs

AbstractThe novel methods for efficient plant regeneration via direct somatic embryogenesis (SE) and SE-mediated transformation system under high concentration of NAA in Ranunculus sceleratus were established. On MS media containing a high concentration of NAA (10.0 mg/L) in the dark, all inoculated explants (root, stem and leaf) formed somatic embryos at high frequencies, respectively, 66.03, 126.47 and 213.63 embryoids per explant, and 100% of the embryoids developed into plantlets on 1/2 MS rooting media. Morphological and histological analyses revealed that SE in R. sceleratus followed a classical pattern. All inoculated explants can be used as receptors for genetic transformation in R. sceleratus, through direct SE-mediated method after Agrobacterium infection. RcLEC1-B, as a marker gene, changed the number and morphology of flower organs and the development of cuticle in R. sceleratus, which indicated that the efficient transgenic system of R. sceleratus was established. To our knowledge, this is the first observation that both direct SE and transgenic transformation system, via induction of a single plant growth regulator, have been successfully constructed in R. sceleratus.

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