Agrobacterium T-DNA mutation causes the loss of GUS expression in transgenic tobacco

1996 ◽  
Vol 15 (6) ◽  
pp. 414-417 ◽  
Author(s):  
Loren C. Stephens ◽  
David J. Hannapel ◽  
Sheryl L. Krell ◽  
Diane R. Shogren
Keyword(s):  
Transgenic Tobacco ◽  
Gus Expression ◽  
Dna Mutation

Agrobacterium T-DNA mutation causes the loss of GUS expression in transgenic tobacco

1996 ◽  
Vol 15 (6) ◽  
pp. 414-417 ◽  
Author(s):  
Loren C. Stephens ◽  
David J. Hannapel ◽  
Sheryl L. Krell ◽  
Diane R. Shogren
Keyword(s):  
Transgenic Tobacco ◽  
Gus Expression ◽  
Dna Mutation

scholarly journals Characteristics of the promoters derived from the single-stranded DNA components of Milk vetch dwarf virus in transgenic tobacco

2005 ◽  
Vol 86 (6) ◽  
pp. 1851-1860 ◽  
Author(s):  
Naomi Shirasawa-Seo ◽  
Yoshitaka Sano ◽  
Shigeo Nakamura ◽  
Taka Murakami ◽  
Shigemi Seo ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Cell Types ◽  
Gus Expression ◽  
Cauliflower Mosaic Virus ◽  
Dwarf Virus ◽  
Promoter Regions ◽  
Meristematic Tissue ◽  
Link Protein ◽  
Gus Reporter ◽  
Milk Vetch Dwarf Virus

Predicted promoter regions of Milk vetch dwarf virus (MDV) components (C1–C11) were isolated and fused with a β-glucuronidase (GUS) reporter gene and the characteristics of the promoters were examined. In transgenic tobacco calli, promoters of MDV C4 (encoding a cell-cycle link protein), C5 and C7 (both encoding unknown proteins), C6 (encoding a nuclear-shuttle protein) and C8 (encoding a movement protein) generated a stronger level of GUS expression than the Cauliflower mosaic virus 35S RNA promoter (P35S). In leaves of transgenic tobacco plants, the promoters of C5 and C8 conferred a level of GUS activity comparable to that of P35S. Histochemical GUS analysis showed that the promoters of C4–C9, the latter encoding a capsid protein, were active in phloem and meristematic tissue. The promoter of C8 was also active in mesophyll and cortex cell types. A low level of activity was found for the promoters of C11, which encodes a master replication-initiator protein (Rep), and C1, C2, C3 and C10, which encode additional Reps, in both transgenic tobacco calli and plants.

scholarly journals Histochemical GUS expression of beta tubulin promoter in transgenic tobacco

2017 ◽  
Vol 16 (17) ◽  
pp. 945-952
Author(s):  
Mubeen Hira ◽  
Bashir Aftab ◽  
Ameen Ayesha ◽  
Masood Ammara ◽  
Raza Shahid
Keyword(s):  
Transgenic Tobacco ◽  
Gus Expression ◽  
Beta Tubulin

Extracellular calmodulin acceleratesrbcS-GUS expression in suspension-cultured transgenic tobacco cell

2000 ◽  
Vol 45 (22) ◽  
pp. 2089-2092 ◽  
Author(s):  
Ligeng Ma ◽  
Junli Zhou ◽  
Suqiao Zhang ◽  
Qiang Liu ◽  
Daye Sun
Keyword(s):  
Transgenic Tobacco ◽  
Gus Expression ◽  
Tobacco Cell ◽  
Extracellular Calmodulin

scholarly journals Expression Pattern of Arabidopsis Thaliana Pollen- and Embryo-Specific Promoter in Transgenic Tobacco Plants

2014 ◽  
Vol 56 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Martin Jopcik ◽  
Ildiko Matusikova ◽  
Jana Moravcikova ◽  
Jana Libantova
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Tobacco ◽  
Expression Profiles ◽  
Pollen Grains ◽  
Apical Part ◽  
Gus Expression ◽  
Transgenic Tobacco Plants ◽  
Specific Expression ◽  
Tobacco Plants ◽  
Biotechnology Research

Abstract Current biotechnology research is focused on tissue-specific expression of genes of interest in plants. Promoters with specific spatial and temporal expression profiles in targeted organisms are in wide use for this. This study investigated whether the Arabidopsis thaliana seed- and pollen-specific promoter MXL maintains its specificity in transgenic tobacco plants. Histochemical analysis revealed that the MXL fusion promoter drives slightly different GUS expression in that heterologous organism. GUS staining was clearly detected in the bicellular stage of pollen development and later in germinating tobacco pollen grains. Unlike in A. thaliana, where the MXL promoter is active during the whole period of embryo development, in tobacco its activity was restricted to a short temporal and spatial window from late-heart to mid-torpedo stages, mainly in the apical part of the developing embryo. These results point to the need to test the expression profiles of heterologous promoters in targeted species before they are used in particular biotechnological programs.

OsRGLP2 promoter derived GUS expression in transgenic tobacco in response to salicylic acid, H2O2, PEG, NaCl and auxins

Plant Gene ◽  
2019 ◽  
Vol 19 ◽  
pp. 100190 ◽  
Author(s):  
Sonia Ayub ◽  
Rabbia Hayat ◽  
Zainab Zainab ◽  
Wasim Akhtar ◽  
Tariq Mahmood
Keyword(s):  
Salicylic Acid ◽  
Transgenic Tobacco ◽  
Gus Expression ◽  
Osrglp2 Promoter

Histochemical GUS Expression of Cotton SPS Promoter in Transgenic Tobacco

2016 ◽  
Vol 8 (1) ◽  
pp. 1-8
Author(s):  
Nadia Iqbal ◽  
Ammara Masood ◽  
Aftab Bashir ◽  
Muhammad Asif
Keyword(s):  
Transgenic Tobacco ◽  
Gus Expression

Co-expression of AGPS and AGPL genes encoded for AGPase from cassava to enhance starch accumulation in transgenic tobacco

2016 ◽  
Vol 14 (2) ◽  
pp. 287-293
Author(s):  
Nguyễn Văn Đoài ◽  
Nguyễn Minh Hồng ◽  
Lê Thu Ngọc ◽  
Nguyễn Thị Thơm ◽  
Nguyễn Đình Trọng ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Higher Plants ◽  
Starch Content ◽  
Genetically Modified Crops ◽  
Starch Accumulation ◽  
35S Promoter ◽  
Effective Strategies ◽  
Plant Expression Vector ◽  
Transgenic Lines ◽  
Cassava Varieties

The AGPase (ADP-Glucose pyrophosphorylase) is one of the ubiquitous enzymes catalyzing the first step in starch biosynthesis. It plays an important role in regulation and adjusts the speed of the entire cycle of glycogen biosynthesis in bacteria and starch in plants. In higher plants, it is a heterotetramer and tetrameric enzyme consisting two large subunits (AGPL) and two small subunits (AGPS) and encoded by two genes. In this paper, both AGPS and AGPL genes were sucessfully isolated from cassava varieties KM140 and deposited in Genbank with accession numbers KU243124 (AGPS) and KU243122 (AGPL), these two genes were fused with P2a and inserted into plant expression vector pBI121 under the control of 35S promoter. The efficient of this construct was tested in transgenic N. tabacum. The presence and expression of AGPS and AGPL in transgenic plants were confirmed by PCR and Western hybridization. The starch content was quantified by the Anthrone method. Transgenic plant analysis indicated that that two targeted genes were expressed simultaneously in several transgenic tobacco lines under the control of CaMV 35S promoter.  The starch contents in 4 analyzed tobacco transgenic lines displays the increase 13-116%  compared to WT plants. These results indicated that the co-expression of AGPS and AGPL is one of effective strategies for enhanced starch production in plant. These results can provide a foundation for developing other genetically modified crops to increase starch accumulation capacity.

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