Transgenic tobacco cell cultures expressing a Trichoderma harzianum endochitinase gene release the enzyme into the medium

2001 ◽  
Vol 20 (1) ◽  
pp. 73-78 ◽  
Author(s):  
A. Brants ◽  
E. D. Earle
Keyword(s):  
Transgenic Tobacco ◽  
Cell Cultures ◽  
Trichoderma Harzianum ◽  
Tobacco Cell ◽  
Endochitinase Gene

Biotransformation of hyoscyamine into scopolamine in transgenic tobacco cell cultures

2007 ◽  
Vol 164 (4) ◽  
pp. 521-524 ◽  
Author(s):  
Elisabeth Moyano ◽  
Javier Palazón ◽  
Mercedes Bonfill ◽  
Lidia Osuna ◽  
Rosa M. Cusidó ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Cell Cultures ◽  
Tobacco Cell

Biotransformation of Metamitron by Human P450 Expressed in Transgenic Tobacco Cell Cultures

2006 ◽  
Vol 41 (3) ◽  
pp. 201-222 ◽  
Author(s):  
MAREN BODE ◽  
MATTHIAS HAAS ◽  
TANYA FAYMONVILLE ◽  
BRIGITTE THIEDE ◽  
INGOLF SCHUPHAN ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Cell Cultures ◽  
Tobacco Cell

Bioreactor strategies for improving production yield and functionality of a recombinant human protein in transgenic tobacco cell cultures

2009 ◽  
Vol 102 (2) ◽  
pp. 508-520 ◽  
Author(s):  
Ting-Kuo Huang ◽  
Michael A. Plesha ◽  
Bryce W. Falk ◽  
Abhaya M. Dandekar ◽  
Karen A. McDonald
Keyword(s):  
Transgenic Tobacco ◽  
Cell Cultures ◽  
Human Protein ◽  
Production Yield ◽  
Tobacco Cell ◽  
Recombinant Human Protein

Site-Directed Homologous Recombination in Tobacco Cell Cultures via Zinc Finger Nucleases

2007 ◽  
pp. 111-113 ◽  
Author(s):  
Charles Q. Cai ◽  
W. Michael Ainley ◽  
Trevor N. Collingwood ◽  
Robbi J. Garrison ◽  
Lisa L. Schulenberg ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Zinc Finger ◽  
Cell Cultures ◽  
Zinc Finger Nucleases ◽  
Tobacco Cell

Effects of Permeabilization on the Biotransformation of Phenylalanine by Immobilized Tobacco Cell Cultures

1989 ◽  
Vol 44 (3-4) ◽  
pp. 249-254 ◽  
Author(s):  
Jochen Berlin ◽  
Bernd Martin ◽  
Jerzy Nowak ◽  
Ludger Witte ◽  
Victor Wray ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Small Proportion ◽  
Culture Medium ◽  
Tobacco Cell ◽  
Permeabilized Cells ◽  
Oxidative Reactions ◽  
A Cell ◽  
Biotransformation Products ◽  
Severe Growth

Abstract Im mobilized tobacco cells excreted only a small proportion of their main secondary metabolite caffeoylputrescine into the culture medium . Experiments designed to release more of the com ­ pound by permeabilization caused the loss of caffeoylputrescine, probably by oxidative reactions. Moreover, rather mild treatments with permeabilizing agents (e.g. n-propanol) resulted in severe growth inhibition. The ability of permeabilized cells to form caffeoylputrescine and other hy-droxycinnam oyl conjugates from phenylalanine decreased considerably even when such cells were still able to metabolize phenylalanine into various ethyl acetate extractable com pounds (e.g. hydroxycinnamic acids and acetophenones). The formation of new biotransformation products suggests that permeabilized cells could be used as a tool for testing the enzymatic capabilities of a cell culture.

scholarly journals Oxidative burst and cell death induced by b-quercinin and zoospores of Phytophthora quercina in tobacco cell cultures

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 446-448 ◽  
Author(s):  
J. Koehl ◽  
E.F. Elstner ◽  
W. Oßwald ◽  
I. Heiser
Keyword(s):  
Cell Death ◽  
Cell Suspension ◽  
Cell Cultures ◽  
Oxidative Burst ◽  
Mode Of Action ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Tobacco Cell ◽  
Tobacco Cell Suspension

Mode of action of β-quercinin, a novel elicitin on tobacco cell suspension cultures (cvs. Bel B and Bel W3) was investigated by measuring the oxidative burst and cell death in these cell cultures. β-quercinin induced an oxidative burst comparable to that excited by zoospores from P. quercina. Adding superoxidedismutase, catalase and diphenyleneiodonium to elicited cell cultures, it could be demonstrated, that the induction of cell death in tobacco cell cultures is not correlated to the oxidative burst.

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