Transcriptional regulation of the KlDLD gene, encoding the mitochondrial enzyme D-lactate ferricytochrome c oxidoreductase in Kluyveromyces lactis : effect of Klhap2 and fog mutations

1998 ◽  
Vol 34 (1) ◽  
pp. 12-20 ◽  
Author(s):  
T. Lodi ◽  
Paola Goffrini ◽  
Iuri Bolondi ◽  
Iliana Ferrero
Keyword(s):  
Transcriptional Regulation ◽  
Kluyveromyces Lactis ◽  
Mitochondrial Enzyme ◽  
Gene Encoding ◽  
Ferricytochrome C

RAG3 gene and transcriptional regulation of the pyruvate decarboxylase gene in Kluyveromyces lactis

1996 ◽  
Vol 20 (4) ◽  
pp. 765-772 ◽  
Author(s):  
C. Prior ◽  
L. Tizzani ◽  
H. Fukuhara ◽  
M. Wésolowski-Louvel
Keyword(s):  
Transcriptional Regulation ◽  
Kluyveromyces Lactis ◽  
Pyruvate Decarboxylase

scholarly journals Improved Production of Heterologous Proteins by a Glucose Repression-Defective Mutant of Kluyveromyces lactis

2004 ◽  
Vol 70 (5) ◽  
pp. 2632-2638 ◽  
Author(s):  
Claudia Donnini ◽  
Francesca Farina ◽  
Barbara Neglia ◽  
Maria Concetta Compagno ◽  
Daniela Uccelletti ◽  
...  
Keyword(s):  
Glucose Repression ◽  
Kluyveromyces Lactis ◽  
Kinase Domain ◽  
Arxula Adeninivorans ◽  
Nucleotide Position ◽  
Heterologous Proteins ◽  
Reporter Protein ◽  
Gene Encoding ◽  
Defective Mutant ◽  
Highly Sensitive

ABSTRACT The secreted production of heterologous proteins in Kluyveromyces lactis was studied. A glucoamylase (GAA) from the yeast Arxula adeninivorans was used as a reporter protein for the study of the secretion efficiencies of several wild-type and mutant strains of K. lactis. The expression of the reporter protein was placed under the control of the strong promoter of the glyceraldehyde-3-phosphate dehydrogenase of Saccharomyces cerevisiae. Among the laboratory strains tested, strain JA6 was the best producer of GAA. Since this strain is known to be highly sensitive to glucose repression and since this is an undesired trait for biomass-oriented applications, we examined heterologous protein production by using glucose repression-defective mutants isolated from this strain. One of them, a mutant carrying a dgr151-1 mutation, showed a significantly improved capability of producing heterologous proteins such as GAA, human serum albumin, and human interleukin-1β compared to the parent strain. dgr151-1 is an allele of RAG5, the gene encoding the only hexokinase present in K. lactis (a homologue of S. cerevisiae HXK2). The mutation in this strain was mapped to nucleotide position +527, resulting in a change from glycine to aspartic acid within the highly conserved kinase domain. Cells carrying the dgr151-1 allele also showed a reduction in N- and O-glycosylation. Therefore, the dgr151 strain may be a promising host for the production of heterologous proteins, especially when the hyperglycosylation of recombinant proteins must be avoided.

Transcriptional regulation by glucose of the yeastPMA1 gene encoding the plasma membrane H+-ATPase

Yeast ◽  
1993 ◽  
Vol 9 (10) ◽  
pp. 1075-1084 ◽  
Author(s):  
Rajini Rao ◽  
Daniela Drummond-Barbosa ◽  
Carolyn W. Slayman
Keyword(s):  
Transcriptional Regulation ◽  
Plasma Membrane ◽  
Gene Encoding
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