Identification of regulatory mutants of Aspergillus aculeatus affected in rhamnogalacturonan hydrolase expression

1996 ◽  
Vol 30 (5) ◽  
pp. 439-446 ◽  
Author(s):  
M. E. G. Suykerbuyk ◽  
P. J. I. van de Vondervoort ◽  
P. J. Schaap ◽  
J. Visser
Keyword(s):  
Aspergillus Aculeatus ◽  
Regulatory Mutants ◽  
Rhamnogalacturonan Hydrolase

scholarly journals GENETICS OF ARGININE BIOSYNTHESIS IN NEUROSPORA CRASSA

Genetics ◽  
1979 ◽  
Vol 93 (3) ◽  
pp. 557-575
Author(s):  
Rowland H Davis
Keyword(s):  
Neurospora Crassa ◽  
Gene Product ◽  
Unit Interval ◽  
Starting Material ◽  
Limited Extent ◽  
Arginine Biosynthesis ◽  
Regulatory Mutants ◽  
Intragenic Complementation ◽  
Translocation Breakpoints ◽  
Bifunctional Gene

ABSTRACT A large number of arginine-requiring mutants of Neurospora was isolated, using a strain already partially impaired in an enzyme of the pathway. Among the mutants, all previously described loci, except one, were represented, and several new loci were defined and mapped. Four groups of mutants were of particular interest. First, thc large group of arg-6 mutants, when tested for intragenic complementation, suggested a bifunctional gene, possibly controlling two steps in ornithine synthesis. This is consistent with the limited enzymic information about this locus. Second, the arg-13 locus was represented by 14 new mutants. All five tested were quite leaky. suggesting that the function controlled by this gene can be rarried out to a limited extent spontaneously or by another gene product. Third, a new locus, arg-14, was defined. It controls a step in ornithine synthesis. It lies in a 1 to 2 map-unit interval between arg-2 and pyr-3 on LG IVR, as shown by mapping in relation tG translocation breakpoints. Fourth, a second new locus whose mutants render the partial mutation in starting material auxotrophic was defined and mapped near the centromere of LG VIL. These new mutants are unable to derepress enzymes of the pathway and may qualify as regulatory mutants.

sae is essential for expression of the staphylococcal adhesins Eap and Emp

Microbiology ◽  
2005 ◽  
Vol 151 (6) ◽  
pp. 1789-1800 ◽  
Author(s):  
Niamh Harraghy ◽  
Jan Kormanec ◽  
Christiane Wolz ◽  
Dagmar Homerova ◽  
Christiane Goerke ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Extracellular Matrix ◽  
Virulence Factor ◽  
Eukaryotic Cells ◽  
Transcription Start Point ◽  
Chronic Infections ◽  
Transcription Start ◽  
Regulatory Mutants ◽  
Global Regulators ◽  
Glucose Analysis

Eap and Emp are two Staphylococcus aureus adhesins initially described as extracellular matrix binding proteins. Eap has since emerged as being important in adherence to and invasion of eukaryotic cells, as well as being described as an immunomodulator and virulence factor in chronic infections. This paper describes the mapping of the transcription start point of the eap and emp promoters. Moreover, using reporter-gene assays and real-time PCR in defined regulatory mutants, environmental conditions and global regulators affecting expression of eap and emp were investigated. Marked differences were found in expression of eap and emp between strain Newman and the 8325 derivatives SH1000 and 8325-4. Moreover, both genes were repressed in the presence of glucose. Analysis of expression of both genes in various regulatory mutants revealed that sarA and agr were involved in their regulation, but the data suggested that there were additional regulators of both genes. In a sae mutant, expression of both genes was severely repressed. sae expression was also reduced in the presence of glucose, suggesting that repression of eap and emp in glucose-containing medium may, in part, be a consequence of a decrease in expression of sae.

An exogalacturonase from Aspergillus aculeatus able to degrade xylogalacturonan

1996 ◽  
Vol 18 (6) ◽  
pp. 707-712 ◽  
Author(s):  
G. Beldman ◽  
L. A. M. van den Broek ◽  
H. A. Schols ◽  
M. J. F. Searle-van Leeuwen ◽  
K. M. J. van Laere ◽  
...  
Keyword(s):  
Aspergillus Aculeatus

Cold-sensitive regulatory mutants of simian virus 40

1980 ◽  
Vol 140 (1) ◽  
pp. 129-142 ◽  
Author(s):  
Daniel DiMaio ◽  
Daniel Nathans
Keyword(s):  
Simian Virus 40 ◽  
Simian Virus ◽  
Regulatory Mutants ◽  
Cold Sensitive

scholarly journals Dereplication Guided Discovery of Secondary Metabolites of Mixed Biosynthetic Origin from Aspergillus aculeatus

Molecules ◽  
2014 ◽  
Vol 19 (8) ◽  
pp. 10898-10921 ◽  
Author(s):  
Lene Petersen ◽  
Casper Hoeck ◽  
Jens Frisvad ◽  
Charlotte Gotfredsen ◽  
Thomas Larsen
Keyword(s):  
Secondary Metabolites ◽  
Aspergillus Aculeatus ◽  
Guided Discovery
Sign in / Sign up

Export Citation Format

Share Document