Molecular analysis of UFE1, a Saccharomyces cerevisiae gene essential for spore formation and vegetative growth

1996 ◽  
Vol 30 (5) ◽  
pp. 396-403 ◽  
Author(s):  
Thomas A. Downing ◽  
R. K. Storms
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Analysis ◽  
Vegetative Growth ◽  
Spore Formation

scholarly journals The Ime2 Protein Kinase Enhances the Disassociation of the Sum1 Repressor from Middle Meiotic Promoters

2009 ◽  
Vol 29 (16) ◽  
pp. 4352-4362 ◽  
Author(s):  
Noreen T. Ahmed ◽  
David Bungard ◽  
Marcus E. Shin ◽  
Michael Moore ◽  
Edward Winter
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Kinase ◽  
Vegetative Growth ◽  
Meiotic Prophase ◽  
Spore Formation ◽  
Cyclin Dependent Kinase ◽  
Dna Elements ◽  
Separate Pathway

ABSTRACT Meiotic development in Saccharomyces cerevisiae (sporulation) is controlled by the sequential transcription of temporally distinct sets of meiosis-specific genes. The induction of middle genes controls exit from meiotic prophase, the completion of the nuclear divisions, and spore formation. Middle promoters are controlled through DNA elements termed middle sporulation elements (MSEs) that are bound by the Sum1 repressor during vegetative growth and by the Ndt80 activator during meiosis. It has been proposed that the induction of middle promoters is controlled by competition between Ndt80 and Sum1 for MSE occupancy. Here, we show that the Sum1 repressor can be removed from middle promoters in meiotic cells independent of Ndt80 expression. This process requires the phosphorylation of Sum1 by the meiosis-specific cyclin-dependent kinase-like kinase Ime2. The deletion of HST1, which encodes a Sir2 paralog that interacts with Sum1, bypasses the requirement for this phosphorylation. These findings suggest that in the presence of Ndt80, Sum1 may be displaced from MSEs through a competition-based mechanism but that in the absence of Ndt80, Sum1 is removed from chromatin in a separate pathway requiring the phosphorylation of Sum1 by Ime2 and the inhibition of Hst1.

scholarly journals The alpha subunit of the Saccharomyces cerevisiae oligosaccharyltransferase complex is essential for vegetative growth of yeast and is homologous to mammalian ribophorin I.

1995 ◽  
Vol 128 (4) ◽  
pp. 525-536 ◽  
Author(s):  
S Silberstein ◽  
P G Collins ◽  
D J Kelleher ◽  
P J Rapiejko ◽  
R Gilmore
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Vegetative Growth ◽  
Functional Characterization ◽  
Glycosylation Site ◽  
Alpha Subunit ◽  
Protein Sequence Analysis ◽  
Exogenous Lipid ◽  
Microsomal Membranes ◽  
High Mannose

Oligosaccharyltransferase mediates the transfer of a preassembled high mannose oligosaccharide from a lipid-linked oligosaccharide donor to consensus glycosylation acceptor sites in newly synthesized proteins in the lumen of the rough endoplasmic reticulum. The Saccharomyces cerevisiae oligosaccharyltransferase is an oligomeric complex composed of six nonidentical subunits (alpha-zeta), two of which are glycoproteins (alpha and beta). The beta and delta subunits of the oligosaccharyltransferase are encoded by the WBP1 and SWP1 genes. Here we describe the functional characterization of the OST1 gene that encodes the alpha subunit of the oligosaccharyltransferase. Protein sequence analysis revealed a significant sequence identity between the Saccharomyces cerevisiae Ost1 protein and ribophorin I, a previously identified subunit of the mammalian oligosaccharyltransferase. A disruption of the OST1 locus was not tolerated in haploid yeast showing that expression of the Ost1 protein is essential for vegetative growth of yeast. An analysis of a series of conditional ost1 mutants demonstrated that defects in the Ost1 protein cause pleiotropic underglycosylation of soluble and membrane-bound glycoproteins at both the permissive and restrictive growth temperatures. Microsomal membranes isolated from ost1 mutant yeast showed marked reductions in the in vitro transfer of high mannose oligosaccharide from exogenous lipid-linked oligosaccharide to a glycosylation site acceptor tripeptide. Microsomal membranes isolated from the ost1 mutants contained elevated amounts of the Kar2 stress-response protein.

Positive control of sporulation-specific genes by the IME1 and IME2 products in Saccharomyces cerevisiae

1990 ◽  
Vol 10 (5) ◽  
pp. 2104-2110
Author(s):  
A P Mitchell ◽  
S E Driscoll ◽  
H E Smith
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Positive Control ◽  
Spore Formation ◽  
Specific Gene ◽  
Heterologous Promoter ◽  
Yeast Saccharomyces Cerevisiae ◽  
Specific Gene Expression ◽  
Rapid Induction

In the yeast Saccharomyces cerevisiae, meiosis and spore formation require the induction of sporulation-specific genes. Two genes are thought to activate the sporulation program: IME1 and IME2 (inducer of meiosis). Both genes are induced upon entry into meiosis, and IME1 is required for IME2 expression. We report here that IME1 is essential for expression of four sporulation-specific genes. In contrast, IME2 is not absolutely essential for expression of the sporulation-specific genes, but contributes to their rapid induction. Expression of IME2 from a heterologous promoter permits the expression of these sporulation-specific genes, meiotic recombination, and spore formation in the absence of IME1. We propose that the IME1 and IME2 products can each activate sporulation-specific genes independently. In addition, the IME1 product stimulates sporulation-specific gene expression indirectly through activation of IME2 expression.

Cloning and molecular analysis of the HAP2 locus: a global regulator of respiratory genes in Saccharomyces cerevisiae

1985 ◽  
Vol 5 (12) ◽  
pp. 3410-3416
Author(s):  
J L Pinkham ◽  
L Guarente
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Analysis ◽  
Carbon Sources ◽  
Direct Integration ◽  
Global Regulator ◽  
Low Levels ◽  
Gene Maps ◽  
Derivatives Of ◽  
In Cells

We report here the cloning of the HAP2 gene, a locus required for the expression of many cytochromes and respiratory functions in Saccharomyces cerevisiae. The cloned sequences were found to direct integration of a marked vector to the chromosomal HAP2 locus, and derivatives of these sequences were shown to yield chromosomal disruptions with a Hap2- phenotype. The gene maps 18 centimorgans centromere proximal to ade5 on the left arm of chromosome VII, distinguishing it from any other previously characterized nuclear petite locus. The HAP2 locus encodes a 1.3-kilobase transcript which is present at extremely low levels and which is derepressed in cells grown in media containing nonfermentable carbon sources. Levels of HAP2 mRNA are not reduced in strains bearing a mutation at the HAP3 locus, which is also required for expression of respiratory functions. Models outlining possible interactions of the products of the HAP2 and HAP3 genes are presented.

scholarly journals Genome-wide bioinformatic and molecular analysis of introns in Saccharomyces cerevisiae

RNA ◽  
1999 ◽  
Vol 5 (2) ◽  
pp. 221-234 ◽  
Author(s):  
MARC SPINGOLA ◽  
LESLIE GRATE ◽  
DAVID HAUSSLER ◽  
MANUEL ARES
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Analysis ◽  
Genome Wide

Biogenesis of Mitochondria: Genetic and molecular analysis of the oli2 region of mitochondrial DNA in Saccharomyces cerevisiae

1984 ◽  
Vol 8 (2) ◽  
pp. 135-146 ◽  
Author(s):  
Charles E. Novitski ◽  
Ian G. Macreadie ◽  
Ronald J. Maxwell ◽  
H. B. Lukins ◽  
Anthony W. Linnane ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mitochondrial Dna ◽  
Molecular Analysis ◽  
Biogenesis Of Mitochondria

Molecular Analysis of the Saccharomyces cerevisiae YHR076w Gene

IUBMB Life ◽  
2000 ◽  
Vol 50 (6) ◽  
pp. 371-377 ◽  
Author(s):  
C. W. Ramos ◽  
U. Güldener ◽  
S. Klein ◽  
J. H. Hegemann ◽  
S. Gonzalez ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Analysis
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