Characterization of the Genes Encoding the Botulinum Neurotoxin Complex in a Strain of Clostridium botulinum Producing Type B and F Neurotoxins

1998 ◽  
Vol 37 (5) ◽  
pp. 312-318 ◽  
Author(s):  
Jesús A. Santos-Buelga ◽  
Matthew D. Collins ◽  
Alison K. East
Keyword(s):  
Clostridium Botulinum ◽  
Botulinum Neurotoxin ◽  
Type B ◽  
Genes Encoding ◽  
Neurotoxin Complex

Isolation and characterization of a neutralizing antibody specific to internalization domain of Clostridium botulinum neurotoxin type B

Toxicon ◽  
2004 ◽  
Vol 44 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Gi-Hyeok Yang ◽  
Kyu-Sik Kim ◽  
Hak-Woo Kim ◽  
Sung Tae Jeong ◽  
Gyung Heng Huh ◽  
...  
Keyword(s):  
Clostridium Botulinum ◽  
Botulinum Neurotoxin ◽  
Neutralizing Antibody ◽  
Type B ◽  
Isolation And Characterization

scholarly journals Crystallization and preliminary crystallographic studies of the HA3 subcomponent of the type B botulinum neurotoxin complex

2011 ◽  
Vol 67 (10) ◽  
pp. 1244-1246
Author(s):  
Kohsuke Nishimura ◽  
Kengo Kitadokoro ◽  
Yuki Takegahara ◽  
Yo Sugawara ◽  
Takuhiro Matsumura ◽  
...  
Keyword(s):  
Botulinum Neurotoxin ◽  
Type B ◽  
Neurotoxin Complex

scholarly journals Multiplex PCR Assay for Detection and Identification of Clostridium botulinum Types A, B, E, and F in Food and Fecal Material

2001 ◽  
Vol 67 (12) ◽  
pp. 5694-5699 ◽  
Author(s):  
Miia Lindström ◽  
Riikka Keto ◽  
Annukka Markkula ◽  
Mari Nevas ◽  
Sebastian Hielm ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Clostridium Botulinum ◽  
Botulinum Neurotoxin ◽  
Bacterial Species ◽  
Type A ◽  
Food Samples ◽  
Type B ◽  
Fecal Material ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

ABSTRACT Botulism is diagnosed by detecting botulinum neurotoxin andClostridium botulinum cells in the patient and in suspected food samples. In this study, a multiplex PCR assay for the detection of Clostridium botulinum types A, B, E, and F in food and fecal material was developed. The method employs four new primer pairs with equal melting temperatures, each being specific to botulinum neurotoxin gene type A, B, E, or F, and enables a simultaneous detection of the four serotypes. A total of 43 C. botulinum strains and 18 strains of other bacterial species were tested. DNA amplification fragments of 782 bp for C. botulinum type A alone, 205 bp for type B alone, 389 bp for type E alone, and 543 bp for type F alone were obtained. Other bacterial species, including C. sporogenes and the nontoxigenic nonproteolytic C. botulinum-like organisms, did not yield a PCR product. Sensitivity of the PCR for types A, E, and F was 102 cells and for type B was 10 cells per reaction mixture. With a two-step enrichment, the detection limit in food and fecal samples varied from 10−2 spore/g for types A, B, and F to 10−1 spore/g of sample material for type E. Of 72 natural food samples investigated, two were shown to contain C. botulinum type A, two contained type B, and one contained type E. The assay is sensitive and specific and provides a marked improvement in the PCR diagnostics of C. botulinum.

scholarly journals Evidence that Plasmid-Borne Botulinum Neurotoxin Type B Genes Are Widespread among Clostridium botulinum Serotype B Strains

PLoS ONE ◽  
2009 ◽  
Vol 4 (3) ◽  
pp. e4829 ◽  
Author(s):  
Giovanna Franciosa ◽  
Antonella Maugliani ◽  
Concetta Scalfaro ◽  
Paolo Aureli
Keyword(s):  
Clostridium Botulinum ◽  
Botulinum Neurotoxin ◽  
Type B ◽  
Serotype B

scholarly journals Genetic Characterization of Clostridium botulinum Associated with Type B Infant Botulism in Japan

2009 ◽  
Vol 47 (9) ◽  
pp. 2720-2728 ◽  
Author(s):  
K. Umeda ◽  
Y. Seto ◽  
T. Kohda ◽  
M. Mukamoto ◽  
S. Kozaki
Keyword(s):  
Clostridium Botulinum ◽  
Genetic Characterization ◽  
Type B ◽  
Infant Botulism
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