A DNA Fragment from the Cyanobacterium Synechocystis sp. PCC 6803 Mediates Gene Expression Inducible by Osmotic Stress in E. coli

1998 ◽  
Vol 37 (2) ◽  
pp. 108-116 ◽  
Author(s):  
Carsten Milkowski *, † , Ariel Quinone ◽  
Martin Hagemann
Keyword(s):  
Gene Expression ◽  
Osmotic Stress ◽  
E Coli ◽  
Dna Fragment ◽  
Synechocystis Sp ◽  
Pcc 6803

Effects of long-term ionic and osmotic stress conditions on photosynthesis in the cyanobacterium Synechocystis sp. PCC 6803

2005 ◽  
Vol 32 (9) ◽  
pp. 807 ◽  
Author(s):  
Saowarath Jantaro ◽  
Paula Mulo ◽  
Tove Jansén ◽  
Aran Incharoensakdi ◽  
Pirkko Mäenpää
Keyword(s):  
Osmotic Stress ◽  
Stress Component ◽  
D1 Protein ◽  
Long Term Effects ◽  
High Concentration ◽  
High Concentrations ◽  
Synechocystis Sp ◽  
Halotolerant Cyanobacterium ◽  
Pcc 6803

Salinity is considered to be one of the most severe problems in worldwide agricultural production, but the published investigations give contradictory results of the effect of ionic and osmotic stresses on photosynthesis. In the present study, long-term effects of both ionic and osmotic stresses, especially on photosynthesis, were investigated using the moderately halotolerant cyanobacterium Synechocystis sp. PCC 6803. Our results show that the PSII activity and the photosynthetic capacity tolerated NaCl but a high concentration of sorbitol completely inhibited both activities. In line with these results, we show that the amount of the D1 protein of PSII was decreased under severe osmotic stress, whereas the levels of PsaA / B and NdhF3 proteins remained unchanged. However, high concentrations of sorbitol stress led to a drastic decrease of both psbA (encoding D1) and psaA (encoding PsaA) transcripts, suggesting that severe osmotic stress may abolish the tight coordination of transcription and translation normally present in bacteria, at least in the case of the psaA gene. Taken together, our results indicate that the osmotic stress component is more detrimental to photosynthesis than the ionic one and, furthermore, under osmotic stress, the D1 protein appears to be the target of this stress treatment.

scholarly journals Characterization of a Cyanobacterial Outer Membrane Protein: An E. Coli Tolc Homologue from Synechocystis Sp. Pcc 6803

2014 ◽  
Vol 106 (2) ◽  
pp. 557a
Author(s):  
Rachna Agarwal ◽  
Stanislav D. Zakharov ◽  
S. Saif Hasan ◽  
Julian P. Whitelegge ◽  
William A. Cramer
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
E Coli ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Gene expression patterns of sulfur starvation in Synechocystis sp. PCC 6803

BMC Genomics ◽  
2008 ◽  
Vol 9 (1) ◽  
pp. 344 ◽  
Author(s):  
Zhigang Zhang ◽  
Ninad D Pendse ◽  
Katherine N Phillips ◽  
James B Cotner ◽  
Arkady Khodursky
Keyword(s):  
Gene Expression ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Sulfur Starvation ◽  
Synechocystis Sp ◽  
Pcc 6803

The histidine kinase Hik33 perceives osmotic stress and cold stress in Synechocystis sp. PCC 6803

2002 ◽  
Vol 46 (4) ◽  
pp. 905-915 ◽  
Author(s):  
Koji Mikami ◽  
Yu Kanesaki ◽  
Iwane Suzuki ◽  
Norio Murata
Keyword(s):  
Osmotic Stress ◽  
Cold Stress ◽  
Histidine Kinase ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Aquaporin AqpZ Is Involved in Cell Volume Regulation and Sensitivity to Osmotic Stress in Synechocystis sp. Strain PCC 6803

2012 ◽  
Vol 194 (24) ◽  
pp. 6828-6836 ◽  
Author(s):  
M. Akai ◽  
K. Onai ◽  
M. Morishita ◽  
H. Mino ◽  
T. Shijuku ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Cell Volume ◽  
Volume Regulation ◽  
Cell Volume Regulation ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Expression of psbA1 gene in Synechocystis sp. PCC 6803 is influenced by CO2

2017 ◽  
Vol 12 (1) ◽  
pp. 156-161
Author(s):  
Ciprian Chiş ◽  
Dalton Carmel ◽  
luliana Chiş ◽  
Aurel Ardelean ◽  
Nicolae Dragos ◽  
...  
Keyword(s):  
Gene Expression ◽  
Photosystem Ii ◽  
Inorganic Carbon ◽  
Microaerobic Conditions ◽  
Almost All ◽  
And Function ◽  
First Time ◽  
Encoding Genes ◽  
Synechocystis Sp ◽  
Pcc 6803

AbstractIn almost all cyanobacteria a small gene family encodes the photosystem II reaction center D1 proteins that play vital roles in the cell. Recently, several types of this protein were functionally characterised and the conditions for their transcript regulation were identified. One of the D1-encoding genes previously believed to be silent is induced by microaerobic conditions. This gene was first described in Synechocystis sp. PCC 6803 as psbA1 encoding the D1 isoform. When Synechocystis cells are shifted from high to ambient level CO2 we recorded an increase in gene expression, similar, but to a lesser extent, to microaerobic conditions. When synthetic air is used to remove the ambient CO2, the induction of the gene is absent. We documented for the first time that expression of a psbA gene is regulated by the inorganic carbon status of the cell. Our conclusion is that both CO2 and microaerobic conditions are independently influencing the expression of psbA1 gene in Synechocystis sp. PCC 6803. Hence, it is crucial to understand the mechanisms of regulation and function of D1 proteins as it could be used for future bio-technological applications as a virtual tool-box for modulating the function of PSII.

scholarly journals Concerted Changes in Gene Expression and Cell Physiology of the Cyanobacterium Synechocystis sp. Strain PCC 6803 during Transitions between Nitrogen and Light-Limited Growth

2011 ◽  
Vol 155 (3) ◽  
pp. 1445-1457 ◽  
Author(s):  
Eneas Aguirre von Wobeser ◽  
Bas W. Ibelings ◽  
Jasper Bok ◽  
Vladimir Krasikov ◽  
Jef Huisman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Physiology ◽  
Limited Growth ◽  
Synechocystis Sp ◽  
Pcc 6803
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